DOI: 10.1126/science.278.5338.605. Sequencing the Human Genome

10.1126/science.278.5338.605

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The human genome project is at the halfway point. The genomes of 11 microbes, Escherichia coli , and yeast are finished, yet the human genome is only 2 percent finished. The scale-up to finish by 2005 presents a significant challenge.

Bibliography

Rowen, L., Mahairas, G., & Hood, L. (1997). Sequencing the Human Genome. Science, 278(5338), 605â607.

Authors 3

Lee Rowen (first)
Gregory Mahairas (additional)
Leroy Hood (additional)

References 19 Referenced 90

The longest submitted sequence to date 1.492 Mb produced by the Olson laboratory at the University of Washington derives from the metabotropic glutamate receptor 8 gene on chromosome 7 (GenBank accession number ). Other sequences >1 Mb include the immunoglobulin λ locus (1.025 Mb accession numbers - and -); the T cell receptor α locus (1.071 Mb accession numbers -); the DiGeorge critical region (1.25 Mb ); and a region of chromosome 19q13 (1.02 Mb ). Numerous sequences in the several hundred kilobase range are now being produced by the large sequencing centers in the United States and elsewhere (for example the Sanger Centre Cambridge UK).
M. D. Adams et al. Nature 377 (suppl.) 3 (1995). EST databases can be accessed via the NCBI BLAST server ().
10.1126/science.270.5244.1945
Cohen D., Chumakov I., Weissenbach J., Nature 366, 698 (1993). (10.1038/366698a0) / Nature by Cohen D. (1993)
Dib C., et al., ibid. 380, 152 (1996). / ibid. by Dib C. (1996)
Shizuya H., et al., Proc. Natl. Acad. Sci U.S.A. 89, 8794 (1992). (10.1073/pnas.89.18.8794) / Proc. Natl. Acad. Sci U.S.A. by Shizuya H. (1992)
The number of reads per kilobase is a function of the desired redundancy of coverage (typically 5- to 10-fold) and the average read length (typically 400 to 800 bases). A comprehensive review of sequencing technology is found in Adams et al. [M. D. Adams C. Fields J. C. Venter Eds. Automated DNA Sequencing and Analysis (Academic Press London 1994)].
Smith L. M., et al., Nature 321, 674 (1986). (10.1038/321674a0) / Nature by Smith L. M. (1986)
Escherichia coli :
10.1126/science.277.5331.1453
; yeast:
Goffeau A., et al., ibid. 274, 546 (1996); / ibid. by Goffeau A. (1996)
; representative microbial genomes: R. D. Fleischmann et al. ibid. 269 496 (1995); C. M. Fraser et al. ibid. 270 397 (1995); C. J. Bult et al. ibid. 273 1058 (1996);
10.1093/nar/24.22.4420
10.1093/dnares/3.3.109
The sequencing status of C. elegans can be accessed from the Drosophila melanogaster sequencing status can be accessed from . There is no central location for the overall status of human and mouse sequencing progress.
Truly reliable estimates of sequence variation rates across the human genome require more data than currently exist. The numbers cited derive from an analysis of 290 kb of the human β T cell receptor locus for which more than one haplotype was sequenced (L. Rowen unpublished results). These variations are annotated in GenBank accession numbers and .
R. Wilson and R. Waterston personal communication.
10.1038/381364a0

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:44 a.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 11:43 p.m.)
Indexed	1 year, 7 months ago (Jan. 13, 2024, 7:02 p.m.)
Issued	27 years, 10 months ago (Oct. 24, 1997)
Published	27 years, 10 months ago (Oct. 24, 1997)
Published Print	27 years, 10 months ago (Oct. 24, 1997)

Funders 0

None

BibTeX

@article{Rowen_1997, title={Sequencing the Human Genome}, volume={278}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.278.5338.605}, DOI={10.1126/science.278.5338.605}, number={5338}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Rowen, Lee and Mahairas, Gregory and Hood, Leroy}, year={1997}, month=oct, pages={605–607} }

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