Evidence for a Role of CRM1 in Signal-Mediated Nuclear Protein Export
10.1126/science.278.5335.141
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Chromosome maintenance region 1 (CRM1), a protein that shares sequence similarities with the karyopherin β family of proteins involved in nuclear import pathway, was shown to form a complex with the leucine-rich nuclear export signal (NES). This interaction was inhibited by leptomycin B, a drug that prevents the function of the CRM1 protein in yeast. To analyze the role of the CRM1-NES interaction in nuclear export, a transport assay based on semipermeabilized cells was developed. In this system, which reconstituted NES-, cytosol-, and energy-dependent nuclear export, leptomycin B specifically blocked export of NES-containing proteins. Thus, the CRM1 protein could act as a NES receptor involved in nuclear protein export.

Bibliography

Ossareh-Nazari, B., Bachelerie, F., & Dargemont, C. (1997). Evidence for a Role of CRM1 in Signal-Mediated Nuclear Protein Export. Science, 278(5335), 141–144.

Authors 3
  1. Batool Ossareh-Nazari (first)
  2. Françoise Bachelerie (additional)
  3. Catherine Dargemont (additional)
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Dates
Type When
Created 23 years, 1 month ago (July 27, 2002, 5:44 a.m.)
Deposited 1 year, 7 months ago (Jan. 13, 2024, 12:02 a.m.)
Indexed 3 weeks, 1 day ago (Aug. 7, 2025, 4:27 p.m.)
Issued 27 years, 10 months ago (Oct. 3, 1997)
Published 27 years, 10 months ago (Oct. 3, 1997)
Published Print 27 years, 10 months ago (Oct. 3, 1997)
Funders 0

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@article{Ossareh_Nazari_1997, title={Evidence for a Role of CRM1 in Signal-Mediated Nuclear Protein Export}, volume={278}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.278.5335.141}, DOI={10.1126/science.278.5335.141}, number={5335}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Ossareh-Nazari, Batool and Bachelerie, Françoise and Dargemont, Catherine}, year={1997}, month=oct, pages={141–144} }