DOI: 10.1126/science.277.5334.1975. Enhancement of Protein Crystal Nucleation by Critical Density Fluctuations

Enhancement of Protein Crystal Nucleation by Critical Density Fluctuations

10.1126/science.277.5334.1975

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Numerical simulations of homogeneous crystal nucleation with a model for globular proteins with short-range attractive interactions showed that the presence of a metastable fluid-fluid critical point drastically changes the pathway for the formation of a crystal nucleus. Close to this critical point, the free-energy barrier for crystal nucleation is strongly reduced and hence, the crystal nucleation rate increases by many orders of magnitude. Because the location of the metastable critical point can be controlled by changing the composition of the solvent, the present work suggests a systematic approach to promote protein crystallization.

Bibliography

Wolde, P. R. ten, & Frenkel, D. (1997). Enhancement of Protein Crystal Nucleation by Critical Density Fluctuations. Science, 277(5334), 1975â1978.

Authors 2

Pieter Rein ten Wolde (first)
Daan Frenkel (additional)

References 32 Referenced 1,297

A. McPherson Preparation and Analysis of Protein Crystals (Krieger Malabar FL 1982).
Durbin S. D., Feher G., Annu. Rev. Phys. Chem. 47, 171 (1996); (10.1146/annurev.physchem.47.1.171) / Annu. Rev. Phys. Chem. by Durbin S. D. (1996)
Rosenberger F., J. Cryst. Growth 166, 40 (1996). (10.1016/0022-0248(95)00921-3) / J. Cryst. Growth by Rosenberger F. (1996)
A. George and W. W. Wilson Acta Crystallogr. D 50 361 (1994). (10.1107/S0907444994001216)
E. G. Richards An Introduction to Physical Properties of Large Molecules in Solution (Cambridge Univ. Press Cambridge 1980).
See for example T. L. Hill An Introduction to Statistical Thermodynamics (Dover New York 1986) chap. 15.
Rosenbaum D., Zamora P. C., Zukoski C. F., Phys. Rev. Lett. 76, 150 (1996). (10.1103/PhysRevLett.76.150) / Phys. Rev. Lett. by Rosenbaum D. (1996)
Rosenbaum D. F., Zukoski C. F., J. Cryst. Growth 169, 752 (1996). (10.1016/S0022-0248(96)00455-1) / J. Cryst. Growth by Rosenbaum D. F. (1996)
10.1016/0021-9797(83)90027-9
10.1209/0295-5075/20/6/015
Hagen M. H. J., Frenkel D., J. Chem. Phys. 101, 4093 (1994). (10.1063/1.467526) / J. Chem. Phys. by Hagen M. H. J. (1994)
Ilett S. M., Orrock A., Poon W. C. K., Pusey P. N., Phys. Rev. E 51, 1344 (1995). (10.1103/PhysRevE.51.1344) / Phys. Rev. E by Ilett S. M. (1995)
10.1039/FD9950100065
Poon W. C. K., Phys. Rev. E 55, 3762 (1997). (10.1103/PhysRevE.55.3762) / Phys. Rev. E by Poon W. C. K. (1997)
Berland C. R., et al., Proc. Natl. Acad. Sci. U.S.A. 89, 1214 (1992); (10.1073/pnas.89.4.1214) / Proc. Natl. Acad. Sci. U.S.A. by Berland C. R. (1992)
Asherie N., Lomakin A., Benedek G. B., Phys. Rev. Lett. 77, 4832 (1996). (10.1103/PhysRevLett.77.4832) / Phys. Rev. Lett. by Asherie N. (1996)
Broide M. L., Tominc T. M., Saxowsky M. D., Phys. Rev. E 53, 6325 (1996). (10.1103/PhysRevE.53.6325) / Phys. Rev. E by Broide M. L. (1996)
Muschol M., Rosenberger F., J. Chem. Phys. 107, 1953 (1997). (10.1063/1.474547) / J. Chem. Phys. by Muschol M. (1997)
McPherson A., J. Biol. Chem. 251, 6300 (1976). (10.1016/S0021-9258(20)81858-4) / J. Biol. Chem. by McPherson A. (1976)
Kose A., Hachisu S., J. Colloid Interface Sci. 55, 487 (1976); (10.1016/0021-9797(76)90059-X) / J. Colloid Interface Sci. by Kose A. (1976)
; C. Smits et al. Phase Transitions 21 157 (1990). (10.1080/01411599008206888)
10.1103/PhysRevLett.75.2714
; J. Chem. Phys. 104 9932 (1996). (10.1063/1.471721)
C. H. Bennett in Diffusion in Solids: Recent Developments A. S. Nowick and J. J. Burton Eds. (Academic Press New York 1975) pp. 73–113;
Chandler D., J. Chem. Phys. 68, 2959 (1978). (10.1063/1.436049) / J. Chem. Phys. by Chandler D. (1978)
See for example K. F. Kelton in Crystal Nucleation in Liquids and Glasses H. Ehrenreich and D. Turnbull Eds. (Academic Press Boston 1991) vol. 45 pp. 75–177. As shown in this reference the constant of proportionality is system-specific. (10.1016/S0081-1947(08)60144-7)
Torrie G. M., Valleau J. P., Chem. Phys. Lett. 28, 578 (1974). (10.1016/0009-2614(74)80109-0) / Chem. Phys. Lett. by Torrie G. M. (1974)
Haas C., Drenth J., J. Cryst. Growth 154, 126 (1995). (10.1016/0022-0248(95)00135-2) / J. Cryst. Growth by Haas C. (1995)
Malkin A. J., McPherson A., ibid. 128, 1232 (1992); / ibid. by Malkin A. J. (1992)
; ibid. 133 29 (1993). (10.1080/10437797.1993.10778806)
Hansen J. P., Verlet L., Phys. Rev. 184, 151 (1969). (10.1103/PhysRev.184.151) / Phys. Rev. by Hansen J. P. (1969)
We thank E. J. Meijer for computing part of the phase diagram in Fig. 1B and A. van Blaaderen M. Dogterom J. Drenth J. M. W. Frenken S. Fraden C. Haas H. N. W. Lekkerkerker B. Smit R. Sear J. T. M. Walraven and C. Zukoski for critical reading of the manuscript. Supported by Scheikundig Onderzoek Nederland and by Fundamenteel Onderzoek der Materie (FOM) with financial aid from Nederlandse Organisatie voor Wetenschappelijk Onderzoek. Computer time was provided by Nationale Computer Faciliteiten.

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:44 a.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 10:51 p.m.)
Indexed	19 hours, 26 minutes ago (Aug. 30, 2025, 1:05 p.m.)
Issued	27 years, 11 months ago (Sept. 26, 1997)
Published	27 years, 11 months ago (Sept. 26, 1997)
Published Print	27 years, 11 months ago (Sept. 26, 1997)

Funders 0

None

BibTeX

@article{Wolde_1997, title={Enhancement of Protein Crystal Nucleation by Critical Density Fluctuations}, volume={277}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.277.5334.1975}, DOI={10.1126/science.277.5334.1975}, number={5334}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Wolde, Pieter Rein ten and Frenkel, Daan}, year={1997}, month=sep, pages={1975–1978} }

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