Cdc25 Mitotic Inducer Targeted by Chk1 DNA Damage Checkpoint Kinase
10.1126/science.277.5331.1495
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Arrest of the cell cycle at the G 2 checkpoint, induced by DNA damage, requires inhibitory phosphorylation of the kinase Cdc2 in both fission yeast and human cells. The kinase Wee1 and the phosphatase Cdc25, which regulate Cdc2 phosphorylation, were evaluated as targets of Chk1, a kinase essential for the checkpoint. Fission yeast cdc2-3w Δcdc25 cells, which express activated Cdc2 and lack Cdc25, were responsive to Wee1 but insensitive to Chk1 and irradiation. Expression of large amounts of Chk1 produced the same phenotype as did loss of the cdc25 gene in cdc2-3w cells. Cdc25 associated with Chk1 in vivo and was phosphorylated when copurified in Chk1 complexes. These findings identify Cdc25, but not Wee1, as a target of the DNA damage checkpoint.

Bibliography

Furnari, B., Rhind, N., & Russell, P. (1997). Cdc25 Mitotic Inducer Targeted by Chk1 DNA Damage Checkpoint Kinase. Science, 277(5331), 1495–1497.

Authors 3
  1. Beth Furnari (first)
  2. Nicholas Rhind (additional)
  3. Paul Russell (additional)
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Dates
Type When
Created 23 years ago (July 27, 2002, 1:50 a.m.)
Deposited 1 year, 7 months ago (Jan. 12, 2024, 5:52 p.m.)
Indexed 1 day, 9 hours ago (Aug. 22, 2025, 12:45 a.m.)
Issued 27 years, 11 months ago (Sept. 5, 1997)
Published 27 years, 11 months ago (Sept. 5, 1997)
Published Print 27 years, 11 months ago (Sept. 5, 1997)
Funders 0

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@article{Furnari_1997, title={Cdc25 Mitotic Inducer Targeted by Chk1 DNA Damage Checkpoint Kinase}, volume={277}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.277.5331.1495}, DOI={10.1126/science.277.5331.1495}, number={5331}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Furnari, Beth and Rhind, Nicholas and Russell, Paul}, year={1997}, month=sep, pages={1495–1497} }