Selective Colorimetric Detection of Polynucleotides Based on the Distance-Dependent Optical Properties of Gold Nanoparticles
10.1126/science.277.5329.1078
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

A highly selective, colorimetric polynucleotide detection method based on mercaptoalkyloligonucleotide-modified gold nanoparticle probes is reported. Introduction of a single-stranded target oligonucleotide (30 bases) into a solution containing the appropriate probes resulted in the formation of a polymeric network of nanoparticles with a concomitant red-to-pinkish/purple color change. Hybridization was facilitated by freezing and thawing of the solutions, and the denaturation of these hybrid materials showed transition temperatures over a narrow range that allowed differentiation of a variety of imperfect targets. Transfer of the hybridization mixture to a reverse-phase silica plate resulted in a blue color upon drying that could be detected visually. The unoptimized system can detect about 10 femtomoles of an oligonucleotide.

Bibliography

Elghanian, R., Storhoff, J. J., Mucic, R. C., Letsinger, R. L., & Mirkin, C. A. (1997). Selective Colorimetric Detection of Polynucleotides Based on the Distance-Dependent Optical Properties of Gold Nanoparticles. Science, 277(5329), 1078–1081.

Authors 5
  1. Robert Elghanian (first)
  2. James J. Storhoff (additional)
  3. Robert C. Mucic (additional)
  4. Robert L. Letsinger (additional)
  5. Chad A. Mirkin (additional)
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  25. Transmission electron microscopy pictures of typical aggregates are available in supplementary material. Similar images can be found in (10).
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  28. We acknowledge support by grants from the National Institute of General Medical Sciences (GM 10265) and the Office of Naval Research (N0014-94-1-0703 and N00014-97-10430) and the Department of Defense (MURI DAAG 55-97-1-0133).
Dates
Type When
Created 23 years ago (July 27, 2002, 5:35 a.m.)
Deposited 1 year, 7 months ago (Jan. 12, 2024, 10:24 p.m.)
Indexed 19 hours, 44 minutes ago (Aug. 21, 2025, 1:37 p.m.)
Issued 28 years ago (Aug. 22, 1997)
Published 28 years ago (Aug. 22, 1997)
Published Print 28 years ago (Aug. 22, 1997)
Funders 0

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@article{Elghanian_1997, title={Selective Colorimetric Detection of Polynucleotides Based on the Distance-Dependent Optical Properties of Gold Nanoparticles}, volume={277}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.277.5329.1078}, DOI={10.1126/science.277.5329.1078}, number={5329}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Elghanian, Robert and Storhoff, James J. and Mucic, Robert C. and Letsinger, Robert L. and Mirkin, Chad A.}, year={1997}, month=aug, pages={1078–1081} }