DOI: 10.1126/science.2756426. Understanding the Anomalous Electrophoresis of Bent DNA Molecules: a Reptation Model

Understanding the Anomalous Electrophoresis of Bent DNA Molecules: a Reptation Model

10.1126/science.2756426

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

In polyacrylamide gel electrophoresis, the retardation of DNA molecules containing regions of intrinsic curvature can be explained by a novel reptation model that includes the elastic free energy of the DNA chain. Computer simulations based on this model give results that reproduce the dependence of anomalous mobility on gel concentration, which is quantified by new experimental data on the mobilities of circularly permuted isomers of kinetoplast DNA fragments. Fitting of the data required allowing for the elasticity of the gel.

Bibliography

Levene, S. D., & Zimm, B. H. (1989). Understanding the Anomalous Electrophoresis of Bent DNA Molecules: a Reptation Model. Science, 245(4916), 396â399.

Authors 2

Stephen D. Levene (first)
Bruno H. Zimm (additional)

References 39 Referenced 57

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 11:59 p.m.)
Indexed	1 year ago (Aug. 12, 2024, 7:16 a.m.)
Issued	36 years, 1 month ago (July 28, 1989)
Published	36 years, 1 month ago (July 28, 1989)
Published Print	36 years, 1 month ago (July 28, 1989)

Funders 0

None

BibTeX

@article{Levene_1989, title={Understanding the Anomalous Electrophoresis of Bent DNA Molecules: a Reptation Model}, volume={245}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2756426}, DOI={10.1126/science.2756426}, number={4916}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Levene, Stephen D. and Zimm, Bruno H.}, year={1989}, month=jul, pages={396–399} }

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