Mixed Self-Assembled Monolayers in Chemical Separations
10.1126/science.275.5296.44
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Chemical separations of many biomolecules and pharmaceuticals are limited by their electrostatic interaction with the surfaces of the separation medium. Mixed self-assembled monolayers of octadecyl and methyl chains organize into a dense, two-dimensionally cross-linked network over the chromatographic silica surface to reduce acid dissociation of the surface silanols. Molecular models predict that two-dimensional cross-linking is sterically possible for pure methylsiloxane monolayers, silicon-29 nuclear magnetic resonance measurements show that cross-linking predominates for mixed monolayers of primarily methylsiloxane, and chromatographic measurements confirm that electrostatic interactions are reduced when the monolayer is primarily methylsiloxane. Chromatographic separation of genetic variants of a highly charged protein, cytochrome c, demonstrates the promise of self-assembled monolayers in separations of biomolecules.

Bibliography

Wirth, M. J., Fairbank, R. W. P., & Fatunmbi, H. O. (1997). Mixed Self-Assembled Monolayers in Chemical Separations. Science, 275(5296), 44–47.

Authors 3
  1. Mary J. Wirth (first)
  2. R. W. Peter Fairbank (additional)
  3. Hafeez O. Fatunmbi (additional)
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Dates
Type When
Created 23 years ago (July 27, 2002, 5:37 a.m.)
Deposited 1 year, 7 months ago (Jan. 12, 2024, 10:35 p.m.)
Indexed 1 year, 3 months ago (May 20, 2024, 3:08 p.m.)
Issued 28 years, 7 months ago (Jan. 3, 1997)
Published 28 years, 7 months ago (Jan. 3, 1997)
Published Print 28 years, 7 months ago (Jan. 3, 1997)
Funders 0

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@article{Wirth_1997, title={Mixed Self-Assembled Monolayers in Chemical Separations}, volume={275}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.275.5296.44}, DOI={10.1126/science.275.5296.44}, number={5296}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Wirth, Mary J. and Fairbank, R. W. Peter and Fatunmbi, Hafeez O.}, year={1997}, month=jan, pages={44–47} }