DOI: 10.1126/science.274.5293.1652. How Proteolysis Drives the Cell Cycle

10.1126/science.274.5293.1652

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Oscillations in the activity of cyclin-dependent kinases (CDKs) promote progression through the eukaryotic cell cycle. This review examines how proteolysis regulates CDK activity—by degrading CDK activators or inhibitors—and also how proteolysis may directly trigger the transition from metaphase to anaphase. Proteolysis during the cell cycle is mediated by two distinct ubiquitin-conjugation pathways. One pathway, requiring CDC34, initiates DNA replication by degrading a CDK inhibitor. The second pathway, involving a large protein complex called the anaphase-promoting complex or cyclosome, initiates chromosome segregation and exit from mitosis by degrading anaphase inhibitors and mitotic cyclins. Proteolysis therefore drives cell cycle progression not only by regulating CDK activity, but by directly influencing chromosome and spindle dynamics.

Bibliography

King, R. W., Deshaies, R. J., Peters, J.-M., & Kirschner, M. W. (1996). How Proteolysis Drives the Cell Cycle. Science, 274(5293), 1652â1659.

Authors 4

Randall W. King (first)
Raymond J. Deshaies (additional)
Jan-Michael Peters (additional)
Marc W. Kirschner (additional)

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Dates

Type	When
Created	23 years ago (July 27, 2002, 5:45 a.m.)
Deposited	1 year ago (Aug. 7, 2024, 7:47 a.m.)
Indexed	2 weeks, 6 days ago (Aug. 5, 2025, 8:18 a.m.)
Issued	28 years, 8 months ago (Dec. 6, 1996)
Published	28 years, 8 months ago (Dec. 6, 1996)
Published Print	28 years, 8 months ago (Dec. 6, 1996)

Funders 0

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BibTeX

@article{King_1996, title={How Proteolysis Drives the Cell Cycle}, volume={274}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.274.5293.1652}, DOI={10.1126/science.274.5293.1652}, number={5293}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={King, Randall W. and Deshaies, Raymond J. and Peters, Jan-Michael and Kirschner, Marc W.}, year={1996}, month=dec, pages={1652–1659} }

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  "abstract": "<jats:p>Oscillations in the activity of cyclin-dependent kinases (CDKs) promote progression through the eukaryotic cell cycle. This review examines how proteolysis regulates CDK activity\u2014by degrading CDK activators or inhibitors\u2014and also how proteolysis may directly trigger the transition from metaphase to anaphase. Proteolysis during the cell cycle is mediated by two distinct ubiquitin-conjugation pathways. One pathway, requiring CDC34, initiates DNA replication by degrading a CDK inhibitor. The second pathway, involving a large protein complex called the anaphase-promoting complex or cyclosome, initiates chromosome segregation and exit from mitosis by degrading anaphase inhibitors and mitotic cyclins. Proteolysis therefore drives cell cycle progression not only by regulating CDK activity, but by directly influencing chromosome and spindle dynamics.</jats:p>",
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