DOI: 10.1126/science.274.5290.1201. Identification of Subunits of the Anaphase-Promoting Complex of <i>Saccharomyces cerevisiae</i>

Identification of Subunits of the Anaphase-Promoting Complex of Saccharomyces cerevisiae

10.1126/science.274.5290.1201

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Entry into anaphase and proteolysis of B-type cyclins depend on a complex containing the tetratricopeptide repeat proteins Cdc16p, Cdc23p, and Cdc27p. This particle, called the anaphase-promoting complex (APC) or cyclosome, functions as a cell cycle-regulated ubiquitin-protein ligase. Two additional subunits of the budding yeast APC were identified: The largest subunit, encoded by the APC1 gene, is conserved between fungi and vertebrates and shows similarity to BIMEp from Aspergillus nidulans . A small heat-inducible subunit is encoded by the CDC26 gene. The yeast APC is a 36 S particle that contains at least seven different proteins.

Bibliography

Zachariae, W., Shin, T. H., Galova, M., Obermaier, B., & Nasmyth, K. (1996). Identification of Subunits of the Anaphase-Promoting Complex of Saccharomyces cerevisiae. Science, 274(5290), 1201â1204.

Authors 5

Wolfgang Zachariae (first)
Tae Ho Shin (additional)
Marta Galova (additional)
Brigitte Obermaier (additional)
Kim Nasmyth (additional)

References 27 Referenced 215

Murray A. W., Solomon M. J., Kirschner M. W., Nature 339, 503 (1989); / Nature by Murray A. W. (1989)
Luca F. C., Shibuya E. K., Dohrman C. E., Ruderman J. V., EMBO J. 10, 4311 (1991). (10.1002/j.1460-2075.1991.tb05009.x) / EMBO J. by Luca F. C. (1991)
10.1002/j.1460-2075.1993.tb05846.x
Dahman C., Diffley J. F. X., Nasmyth K., Curr. Biol. 5, 1257 (1995). (10.1016/S0960-9822(95)00252-1) / Curr. Biol. by Dahman C. (1995)
King R. W., et al., Cell 81, 279 (1995). (10.1016/0092-8674(95)90338-0) / Cell by King R. W. (1995)
10.1091/mbc.6.2.185
Ciechanover A., Cell 79, 13 (1994); (10.1016/0092-8674(94)90396-4) / Cell by Ciechanover A. (1994)
Peters J. M., Trends Biochem. Sci. 19, 377 (1994). (10.1016/0968-0004(94)90115-5) / Trends Biochem. Sci. by Peters J. M. (1994)
Irniger S., Piatti S., Michaelis C., Nasmyth K., Cell 81, 269 (1995). (10.1016/0092-8674(95)90337-2) / Cell by Irniger S. (1995)
Zachariae W., Nasmyth K., Mol. Biol. Cell 7, 791 (1996). (10.1091/mbc.7.5.791) / Mol. Biol. Cell by Zachariae W. (1996)
Hirano T., Hiraoka Y., Yanagida M., J. Cell Biol. 106, 1171 (1988); (10.1083/jcb.106.4.1171) / J. Cell Biol. by Hirano T. (1988)
O'Donnell K. L., Osmani A. H., Osmani S. A., Morris N. R., J. Cell Sci. 99, 711 (1991); (10.1242/jcs.99.4.711) / J. Cell Sci. by O'Donnell K. L. (1991)
Samejima I., Yanagida M., J. Cell Biol. 127, 1655 (1994); (10.1083/jcb.127.6.1655) / J. Cell Biol. by Samejima I. (1994)
10.1016/0092-8674(95)90336-4
Lamb J. R., Michaud W. A., Sikorski R. S., Hieter P. A., EMBO J. 13, 4321 (1994). (10.1002/j.1460-2075.1994.tb06752.x) / EMBO J. by Lamb J. R. (1994)
Holloway S. L., Glotzer M., King R. W., Murray A. W., Cell 73, 1393 (1993). (10.1016/0092-8674(93)90364-V) / Cell by Holloway S. L. (1993)
Funabiki H., et al., Nature 381, 438 (1996). (10.1038/381438a0) / Nature by Funabiki H. (1996)
10.1016/0092-8674(94)90443-X
Akari H., Awane K., Ogawa N., Oshima Y., Mol. Gen. Genet. 231, 329 (1992). (10.1007/BF00279807) / Mol. Gen. Genet. by Akari H. (1992)
Zachariae W. Nasmyth K. unpublished data.
Engle D. B., et al., J. Biol. Chem. 265, 16132 (1990). (10.1016/S0021-9258(17)46198-9) / J. Biol. Chem. by Engle D. B. (1990)
Starborg M., Brundell E., Gell K., Höög C., ibid. 269, 24133 (1994). / ibid. by Starborg M. (1994)
Shin T. H. Nasmyth K. unpublished results.
Ciosk R. Nasmyth K. unpublished results.
10.1126/science.274.5290.1199
Osmani S. A., Engle D. B., Doonan J. H., Morris N. R., Cell 52, 241 (1988); (10.1016/0092-8674(88)90513-2) / Cell by Osmani S. A. (1988)
James S. W., Mirabito P. M., Scacheri P. C., Morris N. R., J. Cell Sci. 108, 3485 (1995). (10.1242/jcs.108.11.3485) / J. Cell Sci. by James S. W. (1995)

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:35 a.m.)
Deposited	1 year ago (Aug. 7, 2024, 7:32 a.m.)
Indexed	3 months, 1 week ago (May 21, 2025, 7:43 p.m.)
Issued	28 years, 9 months ago (Nov. 15, 1996)
Published	28 years, 9 months ago (Nov. 15, 1996)
Published Print	28 years, 9 months ago (Nov. 15, 1996)

Funders 0

None

BibTeX

@article{Zachariae_1996, title={Identification of Subunits of the Anaphase-Promoting Complex of Saccharomyces cerevisiae}, volume={274}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.274.5290.1201}, DOI={10.1126/science.274.5290.1201}, number={5290}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Zachariae, Wolfgang and Shin, Tae Ho and Galova, Marta and Obermaier, Brigitte and Nasmyth, Kim}, year={1996}, month=nov, pages={1201–1204} }

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  "abstract": "<jats:p>\n            Entry into anaphase and proteolysis of B-type cyclins depend on a complex containing the tetratricopeptide repeat proteins Cdc16p, Cdc23p, and Cdc27p. This particle, called the anaphase-promoting complex (APC) or cyclosome, functions as a cell cycle-regulated ubiquitin-protein ligase. Two additional subunits of the budding yeast APC were identified: The largest subunit, encoded by the\n            <jats:italic>APC1</jats:italic>\n            gene, is conserved between fungi and vertebrates and shows similarity to BIMEp from\n            <jats:italic>Aspergillus nidulans</jats:italic>\n            . A small heat-inducible subunit is encoded by the\n            <jats:italic>CDC26</jats:italic>\n            gene. The yeast APC is a 36\n            <jats:italic>S</jats:italic>\n            particle that contains at least seven different proteins.\n          </jats:p>",
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