DOI: 10.1126/science.274.5289.966. Three-Dimensional Imaging of Single Molecules Solvated in Pores of Poly(acrylamide) Gels

Three-Dimensional Imaging of Single Molecules Solvated in Pores of Poly(acrylamide) Gels

10.1126/science.274.5289.966

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Individual fluorescent molecules and individual singly labeled proteins were observed in the water-filled pores of poly(acrylamide) gels by far-field microscopy. Brownian motion was markedly reduced by the gel framework, thus enabling extended study of single fluorophores in aqueous environments. A highly axially dependent laser field was used both to excite the fluorophores and to image the molecules in three dimensions. Single molecules were followed as they moved within and through the porous gel structure. In contrast to dry polymeric hosts, these water-based gels may form a useful medium for single-molecule studies of biological systems in vitro.

Bibliography

Dickson, R. M., Norris, D. J., Tzeng, Y.-L., & Moerner, W. E. (1996). Three-Dimensional Imaging of Single Molecules Solvated in Pores of Poly(acrylamide) Gels. Science, 274(5289), 966â968.

Authors 4

Robert M. Dickson (first)
D. J. Norris (additional)
Yih-Ling Tzeng (additional)
W. E. Moerner (additional)

References 30 Referenced 336

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Shera E. B., Seitzinger N. K., Davis L. M., Keller R. A., Soper S. A., Chem. Phys. Lett. 174, 553 (1990). (10.1016/0009-2614(90)85485-U) / Chem. Phys. Lett. by Shera E. B. (1990)
Wilkerson C. W., Goodwin P. M., Ambrose W. P., Martin J. C., Keller R. A., Appl. Phys. Lett. 62, 2030 (1993). (10.1063/1.109495) / Appl. Phys. Lett. by Wilkerson C. W. (1993)
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Haab B. B., Mathies R. A., Anal. Chem. 67, 3253 (1995). (10.1021/ac00114a023) / Anal. Chem. by Haab B. B. (1995)
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Jasny J., et al., Rev. Sci. Instrum. 67, 1425 (1996). (10.1063/1.1146868) / Rev. Sci. Instrum. by Jasny J. (1996)
Schmidt Th., Schutz G. J., Baumgartner W., Gruber H. J., Schindler H., Proc. Natl. Acad. Sci. U.S.A. 93, 2926 (1996). (10.1073/pnas.93.7.2926) / Proc. Natl. Acad. Sci. U.S.A. by Schmidt Th. (1996)
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10.1126/science.262.5138.1422
Trautman J. K., Macklin J. J., Brus L. E., Betzig E., Nature 369, 40 (1994). (10.1038/369040a0) / Nature by Trautman J. K. (1994)
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Xie X. S. Dunn R. C. ibid. p. 361.
Macklin J. J., Trautman J. K., Harris T. D., Brus L. E., ibid. 272, 255 (1996). / ibid. by Macklin J. J. (1996)
Trautman J. K., Macklin J. J., Chem. Phys. 205, 221 (1996). (10.1016/0301-0104(95)00391-6) / Chem. Phys. by Trautman J. K. (1996)
Meixner A. J., Zeisel D., Bopp M. A., Tarrach G., Opt. Eng. 34, 2324 (1995). (10.1117/12.200620) / Opt. Eng. by Meixner A. J. (1995)
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Vale R. D., et al., ibid. 380, 451 (1996). / ibid. by Vale R. D. (1996)
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10.1021/ma00053a035

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:35 a.m.)
Deposited	1 year ago (Aug. 7, 2024, 7:37 a.m.)
Indexed	1 month, 3 weeks ago (July 1, 2025, 6:29 a.m.)
Issued	28 years, 9 months ago (Nov. 8, 1996)
Published	28 years, 9 months ago (Nov. 8, 1996)
Published Print	28 years, 9 months ago (Nov. 8, 1996)

Funders 0

None

BibTeX

@article{Dickson_1996, title={Three-Dimensional Imaging of Single Molecules Solvated in Pores of Poly(acrylamide) Gels}, volume={274}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.274.5289.966}, DOI={10.1126/science.274.5289.966}, number={5289}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Dickson, Robert M. and Norris, D. J. and Tzeng, Yih-Ling and Moerner, W. E.}, year={1996}, month=nov, pages={966–968} }

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