DOI: 10.1126/science.274.5287.624. Kap104p: A Karyopherin Involved in the Nuclear Transport of Messenger RNA Binding Proteins

Kap104p: A Karyopherin Involved in the Nuclear Transport of Messenger RNA Binding Proteins

10.1126/science.274.5287.624

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A cytosolic yeast karyopherin, Kap104p, was isolated and shown to function in the nuclear import of a specific class of proteins. The protein bound directly to repeat-containing nucleoporins and to a cytosolic pool of two nuclear messenger RNA (mRNA) binding proteins, Nab2p and Nab4p. Depletion of Kap104p resulted in a rapid shift of Nab2p from the nucleus to the cytoplasm without affecting the localization of other nuclear proteins tested. This finding suggests that the major function of Kap104p lies in returning mRNA binding proteins to the nucleus after mRNA export.

Bibliography

Aitchison, J. D., Blobel, G., & Rout, M. P. (1996). Kap104p: A Karyopherin Involved in the Nuclear Transport of Messenger RNA Binding Proteins. Science, 274(5287), 624â627.

Authors 3

John D. Aitchison (first)
Günter Blobel (additional)
Michael P. Rout (additional)

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Dates

Type	When
Created	23 years ago (July 27, 2002, 5:45 a.m.)
Deposited	1 year ago (Aug. 7, 2024, 7:22 a.m.)
Indexed	2 weeks, 4 days ago (Aug. 5, 2025, 8:50 a.m.)
Issued	28 years, 9 months ago (Oct. 25, 1996)
Published	28 years, 9 months ago (Oct. 25, 1996)
Published Print	28 years, 9 months ago (Oct. 25, 1996)

Funders 0

None

BibTeX

@article{Aitchison_1996, title={Kap104p: A Karyopherin Involved in the Nuclear Transport of Messenger RNA Binding Proteins}, volume={274}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.274.5287.624}, DOI={10.1126/science.274.5287.624}, number={5287}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Aitchison, John D. and Blobel, Günter and Rout, Michael P.}, year={1996}, month=oct, pages={624–627} }

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      "doi-asserted-by": "crossref",
      "first-page": "1287",
      "DOI": "10.1083/jcb.118.6.1287",
      "volume": "118",
      "author": "Izaurralde E.",
      "year": "1992",
      "unstructured": "Izaurralde E., Stepinski J., Darzynkiewicz E., Mattaj I. W., J. Cell Biol. 118, 1287 (1992);",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "e_1_3_1_19_4",
      "doi-asserted-by": "crossref",
      "first-page": "573",
      "DOI": "10.1002/j.1460-2075.1993.tb05689.x",
      "volume": "12",
      "author": "Fischer U.",
      "year": "1993",
      "unstructured": "Fischer U., Sumpter V., Sekine M., Satoh T., Luhrmann R., EMBO J. 12, 573 (1993);",
      "journal-title": "EMBO J."
    },
    {
      "doi-asserted-by": "publisher",
      "key": "e_1_3_1_19_5",
      "DOI": "10.1016/0092-8674(95)90435-2"
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      "doi-asserted-by": "publisher",
      "key": "e_1_3_1_20_2",
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      "key": "e_1_3_1_20_3",
      "doi-asserted-by": "crossref",
      "first-page": "545",
      "DOI": "10.1242/jcs.108.2.545",
      "volume": "108",
      "author": "Weighardt F.",
      "year": "1995",
      "unstructured": "Weighardt F., Biamonti G., Riva S., J. Cell Sci. 108, 545 (1995).",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "e_1_3_1_21_2",
      "first-page": "5640",
      "volume": "12",
      "author": "Yano R.",
      "year": "1992",
      "unstructured": "Yano R., Oakes M., Yamaghishi M., Dodd J. A., Nomura M., Mol. Cell. Biol. 12, 5640 (1992);",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "e_1_3_1_21_3",
      "doi-asserted-by": "crossref",
      "first-page": "6880",
      "DOI": "10.1073/pnas.91.15.6880",
      "volume": "91",
      "author": "Yano R.",
      "year": "1994",
      "unstructured": "Yano R., Oakes M. L., Tabb M. M., Nomura M., Proc. Natl. Acad. Sci. U.S.A. 91, 6880 (1994);",
      "journal-title": "Proc. Natl. Acad. Sci. U.S.A."
    },
    {
      "key": "e_1_3_1_21_4",
      "doi-asserted-by": "crossref",
      "first-page": "619",
      "DOI": "10.1083/jcb.126.3.619",
      "volume": "126",
      "author": "Belanger K. D.",
      "year": "1994",
      "unstructured": "Belanger K. D., Kenna M. A., Wei S., Davis L. I., J. Cell Biol. 126, 619 (1994);",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "e_1_3_1_21_5",
      "doi-asserted-by": "crossref",
      "first-page": "16499",
      "DOI": "10.1074/jbc.270.28.16499",
      "volume": "270",
      "author": "Enenkel C.",
      "year": "1995",
      "unstructured": "Enenkel C., Blobel G., Rexach M., J. Biol. Chem. 270, 16499 (1995).",
      "journal-title": "J. Biol. Chem."
    },
    {
      "doi-asserted-by": "publisher",
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    },
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      "doi-asserted-by": "crossref",
      "first-page": "7059",
      "DOI": "10.1073/pnas.93.14.7059",
      "volume": "93",
      "author": "Morianu J.",
      "year": "1996",
      "unstructured": "Morianu J., Blobel G., Radu A., Proc. Natl. Acad. Sci. U.S.A. 93, 7059 (1996)]. Although the acidic amino acids of the central RanGTP binding site of karyopherin \u03b2 (amino acids 333 to 340) are conserved in Kap95p (amino acids 338 to 344), the acidic stretch of Kap104p, which is aligned in the primary structure, is not present within its predicted HEAT motifs.",
      "journal-title": "Proc. Natl. Acad. Sci. U.S.A."
    },
    {
      "key": "e_1_3_1_27_2",
      "doi-asserted-by": "crossref",
      "first-page": "1659",
      "DOI": "10.1083/jcb.131.6.1659",
      "volume": "131",
      "author": "Aitchison J. D.",
      "year": "1995",
      "unstructured": "Aitchison J. D., Blobel G., Rout M. P., J. Cell Biol. 131, 1659 (1995).",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "e_1_3_1_28_2",
      "doi-asserted-by": "crossref",
      "first-page": "979",
      "DOI": "10.1016/0092-8674(90)90063-K",
      "volume": "61",
      "author": "Nehrbass U.",
      "year": "1990",
      "unstructured": "Nehrbass U., et al., Cell 61, 979 (1990).",
      "journal-title": "Cell"
    },
    {
      "key": "e_1_3_1_29_2",
      "doi-asserted-by": "crossref",
      "first-page": "771",
      "DOI": "10.1083/jcb.123.4.771",
      "volume": "123",
      "author": "Rout M. P.",
      "year": "1993",
      "unstructured": "Rout M. P., Blobel G., J. Cell Biol. 123, 771 (1993);",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "e_1_3_1_29_3",
      "first-page": "1913",
      "volume": "111",
      "author": "Rout M. P.",
      "year": "1990",
      "unstructured": "Rout M. P., Kilmartin J. V., ibid. 111, 1913 (1990).",
      "journal-title": "ibid."
    },
    {
      "unstructured": "Aitchison J. D. Blobel G. Rout M. P. data not shown.",
      "key": "e_1_3_1_30_2"
    },
    {
      "unstructured": "Rout M. P. Blobel G. Aitchison J. in preparation.",
      "key": "e_1_3_1_31_2"
    },
    {
      "key": "e_1_3_1_34_2",
      "first-page": "2730",
      "volume": "13",
      "author": "Anderson J. T.",
      "year": "1993",
      "unstructured": "Anderson J. T., Wilson S. M., Datar K. V., Swanson M. S., Mol. Cell. Biol. 13, 2730 (1993);",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "e_1_3_1_34_3",
      "doi-asserted-by": "crossref",
      "first-page": "103",
      "DOI": "10.1093/genetics/142.1.103",
      "volume": "142",
      "author": "Henry M.",
      "year": "1996",
      "unstructured": "Henry M., Borland C. Z., Bossie M., Silver P. A., Genetics 142, 103 (1996).",
      "journal-title": "Genetics"
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      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1083/jcb.113.1.1",
      "volume": "113",
      "author": "Lee W. C.",
      "year": "1991",
      "unstructured": "Lee W. C., Xue Z. X., Melese T., J. Cell Biol. 113, 1 (1991);",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "e_1_3_1_37_3",
      "first-page": "17",
      "volume": "107",
      "author": "Aris J. P.",
      "year": "1988",
      "unstructured": "Aris J. P., Blobel G., ibid. 107, 17 (1988);",
      "journal-title": "ibid."
    },
    {
      "key": "e_1_3_1_37_4",
      "doi-asserted-by": "crossref",
      "first-page": "875",
      "DOI": "10.1091/mbc.3.8.875",
      "volume": "3",
      "author": "Bossie M. A.",
      "year": "1992",
      "unstructured": "Bossie M. A., DeHoratius C., Barcelo G., Silver P.. Mol. Biol. Cell 3, 875 (1992).",
      "journal-title": "Mol. Biol. Cell"
    },
    {
      "unstructured": "Bonifaci N. Blobel G. personal communication.",
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      "doi-asserted-by": "crossref",
      "first-page": "1133",
      "DOI": "10.1083/jcb.131.5.1133",
      "volume": "131",
      "author": "Aitchison J. D.",
      "year": "1995",
      "unstructured": "Aitchison J. D., Rout M. P., Marelli M., Blobel G., Wozniak R. W., J. Cell Biol. 131, 1133 (1995).",
      "journal-title": "J. Cell Biol."
    },
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      "DOI": "10.1074/jbc.270.32.19017",
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      "author": "Kraemer D. M.",
      "year": "1995",
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