DOI: 10.1126/science.274.5287.614. An Asymmetric Model for the Nucleosome: A Binding Site for Linker Histones Inside the DNA Gyres

An Asymmetric Model for the Nucleosome: A Binding Site for Linker Histones Inside the DNA Gyres

10.1126/science.274.5287.614

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Histone-DNA contacts within a nucleosome influence the function of trans-acting factors and the molecular machines required to activate the transcription process. The internal architecture of a positioned nucleosome has now been probed with the use of photoactivatable cross-linking reagents to determine the placement of histones along the DNA molecule. A model for the nucleosome is proposed in which the winged-helix domain of the linker histone is asymmetrically located inside the gyres of DNA that also wrap around the core histones. This domain extends the path of the protein superhelix to one side of the core particle.

Bibliography

Pruss, D., Bartholomew, B., Persinger, J., Hayes, J., Arents, G., Moudrianakis, E. N., & Wolffe, A. P. (1996). An Asymmetric Model for the Nucleosome: A Binding Site for Linker Histones Inside the DNA Gyres. Science, 274(5287), 614â617.

Authors 7

Dmitry Pruss (first)
Blaine Bartholomew (additional)
Jim Persinger (additional)
Jeffrey Hayes (additional)
Gina Arents (additional)
Evangelos N. Moudrianakis (additional)
Alan P. Wolffe (additional)

References 40 Referenced 149

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10.1016/S0092-8674(00)81063-6
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10.1126/science.184.4139.868
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Clark K. L., Halay E. H., Lai E., Burley S. K., ibid. 364, 412 (1993). / ibid. by Clark K. L. (1993)
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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:37 a.m.)
Deposited	1 year ago (Aug. 7, 2024, 7:21 a.m.)
Indexed	3 weeks, 3 days ago (Aug. 6, 2025, 8:41 a.m.)
Issued	28 years, 10 months ago (Oct. 25, 1996)
Published	28 years, 10 months ago (Oct. 25, 1996)
Published Print	28 years, 10 months ago (Oct. 25, 1996)

Funders 0

None

BibTeX

@article{Pruss_1996, title={An Asymmetric Model for the Nucleosome: A Binding Site for Linker Histones Inside the DNA Gyres}, volume={274}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.274.5287.614}, DOI={10.1126/science.274.5287.614}, number={5287}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Pruss, Dmitry and Bartholomew, Blaine and Persinger, Jim and Hayes, Jeffrey and Arents, Gina and Moudrianakis, Evangelos N. and Wolffe, Alan P.}, year={1996}, month=oct, pages={614–617} }

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