DOI: 10.1126/science.274.5285.249. Cdc13p: A Single-Strand Telomeric DNA-Binding Protein with a Dual Role in Yeast Telomere Maintenance

Cdc13p: A Single-Strand Telomeric DNA-Binding Protein with a Dual Role in Yeast Telomere Maintenance

10.1126/science.274.5285.249

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The CDC13 gene has previously been implicated in the maintenance of telomere integrity in Saccharomyces cerevisiae . With the use of two classes of mutations, here it is shown that CDC13 has two discrete roles at the telomere. The cdc13-2 est mutation perturbs a function required in vivo for telomerase regulation but not in vitro for enzyme activity, whereas cdc13-1 ts defines a separate essential role at the telomere. In vitro, purified Cdc13p binds to single-strand yeast telomeric DNA. Therefore, Cdc13p is a telomere-binding protein required to protect the telomere and mediate access of telomerase to the chromosomal terminus.

Bibliography

Nugent, C. I., Hughes, T. R., Lue, N. F., & Lundblad, V. (1996). Cdc13p: A Single-Strand Telomeric DNA-Binding Protein with a Dual Role in Yeast Telomere Maintenance. Science, 274(5285), 249â252.

Authors 4

Constance I. Nugent (first)
Timothy R. Hughes (additional)
Neal F. Lue (additional)
Victoria Lundblad (additional)

References 35 Referenced 480

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:35 a.m.)
Deposited	1 year ago (Aug. 7, 2024, 7:38 a.m.)
Indexed	3 weeks, 2 days ago (Aug. 5, 2025, 8:52 a.m.)
Issued	28 years, 10 months ago (Oct. 11, 1996)
Published	28 years, 10 months ago (Oct. 11, 1996)
Published Print	28 years, 10 months ago (Oct. 11, 1996)

Funders 0

None

BibTeX

@article{Nugent_1996, title={Cdc13p: A Single-Strand Telomeric DNA-Binding Protein with a Dual Role in Yeast Telomere Maintenance}, volume={274}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.274.5285.249}, DOI={10.1126/science.274.5285.249}, number={5285}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Nugent, Constance I. and Hughes, Timothy R. and Lue, Neal F. and Lundblad, Victoria}, year={1996}, month=oct, pages={249–252} }

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