DOI: 10.1126/science.274.5285.239. Role of GTP Hydrolysis in Fission of Caveolae Directly from Plasma Membranes

Role of GTP Hydrolysis in Fission of Caveolae Directly from Plasma Membranes

10.1126/science.274.5285.239

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Caveolae are specialized invaginated cell surface microdomains of undefined function. A cell-free system that reconstituted fission of caveolae from lung endothelial plasma membranes was developed. Addition of cytosol and the hydrolysis of guanosine triphosphate (GTP) induced caveolar fission. The budded caveolae were isolated as vesicles rich in caveolin and the sialoglycolipid G M1 but not glycosyl-phosphatidylinositol (GPI)-anchored proteins. These vesicles contained the molecular machinery for endocytosis and transcytosis. In permeabilized endothelial cells, GTP stimulated, whereas GTPγS prevented, caveolar budding and endocytosis of the cholera toxin B chain to endosomes. Thus, caveolae may bud to form discrete carrier vesicles that participate in membrane trafficking.

Bibliography

Schnitzer, J. E., Oh, P., & McIntosh, D. P. (1996). Role of GTP Hydrolysis in Fission of Caveolae Directly from Plasma Membranes. Science, 274(5285), 239â242.

Authors 3

Jan E. Schnitzer (first)
Phil Oh (additional)
Deirdre P. McIntosh (additional)

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Dates

Type	When
Created	23 years ago (July 27, 2002, 5:45 a.m.)
Deposited	1 year ago (Aug. 7, 2024, 7:37 a.m.)
Indexed	3 days, 4 hours ago (Aug. 23, 2025, 12:59 a.m.)
Issued	28 years, 10 months ago (Oct. 11, 1996)
Published	28 years, 10 months ago (Oct. 11, 1996)
Published Print	28 years, 10 months ago (Oct. 11, 1996)

Funders 0

None

BibTeX

@article{Schnitzer_1996, title={Role of GTP Hydrolysis in Fission of Caveolae Directly from Plasma Membranes}, volume={274}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.274.5285.239}, DOI={10.1126/science.274.5285.239}, number={5285}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Schnitzer, Jan E. and Oh, Phil and McIntosh, Deirdre P.}, year={1996}, month=oct, pages={239–242} }

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