DOI: 10.1126/science.273.5276.797. Diffusional Mobility of Golgi Proteins in Membranes of Living Cells

Diffusional Mobility of Golgi Proteins in Membranes of Living Cells

10.1126/science.273.5276.797

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The mechanism by which Golgi membrane proteins are retained within the Golgi complex in the midst of a continuous flow of protein and lipid is not yet understood. The diffusional mobilities of mammalian Golgi membrane proteins fused with green fluorescent protein from Aequorea victoria were measured in living HeLa cells with the fluorescence photobleaching recovery technique. The diffusion coefficients ranged from 3 × 10 −9 square centimeters per second to 5 × 10 −9 square centimeters per second, with greater than 90 percent of the chimeric proteins mobile. Extensive lateral diffusion of the chimeric proteins occurred between Golgi stacks. Thus, the chimeras diffuse rapidly and freely in Golgi membranes, which suggests that Golgi targeting and retention of these molecules does not depend on protein immobilization.

Bibliography

Cole, N. B., Smith, C. L., Sciaky, N., Terasaki, M., Edidin, M., & Lippincott-Schwartz, J. (1996). Diffusional Mobility of Golgi Proteins in Membranes of Living Cells. Science, 273(5276), 797â801.

Authors 6

Nelson B. Cole (first)
Carolyn L. Smith (additional)
Noah Sciaky (additional)
Mark Terasaki (additional)
Michael Edidin (additional)
Jennifer Lippincott-Schwartz (additional)

References 36 Referenced 433

BECKERS CJMSEMI-INTACT CELLS PERMEABLE TO MACROMOLECULES - USE IN RECONSTITUTION OF PROTEIN-TRANSPORT FROM THE ENDOPLASMIC-RETICULUM TO THE GOLGI-COMPLEXCELL 50, 523 (1987). (10.1016/0092-8674(87)90025-0) / CELL / SEMI-INTACT CELLS PERMEABLE TO MACROMOLECULES - USE IN RECONSTITUTION OF PROTEIN-TRANSPORT FROM THE ENDOPLASMIC-RETICULUM TO THE GOLGI-COMPLEX by BECKERS CJM (1987)
10.1126/science.8362242
10.1126/science.8303295
COOPER M.S.TUBULOVESICULAR PROCESSES EMERGE FROM TRANS-GOLGI CISTERNAE, EXTEND ALONG MICROTUBULES, AND INTERLINK ADJACENT TRANS-GOLGI ELEMENTS INTO A RETICULUMCELL 61, 135 (1990). (10.1016/0092-8674(90)90221-Y) / CELL / TUBULOVESICULAR PROCESSES EMERGE FROM TRANS-GOLGI CISTERNAE, EXTEND ALONG MICROTUBULES, AND INTERLINK ADJACENT TRANS-GOLGI ELEMENTS INTO A RETICULUM by COOPER M.S. (1990)
DONALDSON J.G.DISSOCIATION OF A 110-KD PERIPHERAL MEMBRANE-PROTEIN FROM THE GOLGI-APPARATUS IS AN EARLY EVENT IN BREFELDIN-A ACTIONJOURNAL OF CELL BIOLOGY 111, 2295 (1990). (10.1083/jcb.111.6.2295) / JOURNAL OF CELL BIOLOGY / DISSOCIATION OF A 110-KD PERIPHERAL MEMBRANE-PROTEIN FROM THE GOLGI-APPARATUS IS AN EARLY EVENT IN BREFELDIN-A ACTION by DONALDSON J.G. (1990)
DONALDSON J.G.GUANINE-NUCLEOTIDES MODULATE THE EFFECTS OF BREFELDIN-A IN SEMIPERMEABLE CELLS - REGULATION OF THE ASSOCIATION OF A 110-KD PERIPHERAL MEMBRANE-PROTEIN WITH THE GOLGI-APPARATUSJOURNAL OF CELL BIOLOGY 112, 579 (1991). (10.1083/jcb.112.4.579) / JOURNAL OF CELL BIOLOGY / GUANINE-NUCLEOTIDES MODULATE THE EFFECTS OF BREFELDIN-A IN SEMIPERMEABLE CELLS - REGULATION OF THE ASSOCIATION OF A 110-KD PERIPHERAL MEMBRANE-PROTEIN WITH THE GOLGI-APPARATUS by DONALDSON J.G. (1991)
EDIDIN M, MOBILITY PROXIMITY B1994109. / MOBILITY PROXIMITY B by EDIDIN M (1994)
GRIFFITHS GTHE DYNAMIC NATURE OF THE GOLGI-COMPLEXJOURNAL OF CELL BIOLOGY 108, 277 (1989). (10.1083/jcb.108.2.277) / JOURNAL OF CELL BIOLOGY / THE DYNAMIC NATURE OF THE GOLGI-COMPLEX by GRIFFITHS G (1989)
HEIM RIMPROVED GREEN FLUORESCENCENATURE 373, 663 (1995). (10.1038/373663b0) / NATURE / IMPROVED GREEN FLUORESCENCE by HEIM R (1995)
HSU V.W.A BREFELDIN-A-LIKE PHENOTYPE IS INDUCED BY THE OVEREXPRESSION OF A HUMAN ERD-2-LIKE PROTEIN, ELP-1CELL 69, 625 (1992). (10.1016/0092-8674(92)90226-3) / CELL / A BREFELDIN-A-LIKE PHENOTYPE IS INDUCED BY THE OVEREXPRESSION OF A HUMAN ERD-2-LIKE PROTEIN, ELP-1 by HSU V.W. (1992)
JACKSON M.R.RETRIEVAL OF TRANSMEMBRANE PROTEINS TO THE ENDOPLASMIC-RETICULUMJOURNAL OF CELL BIOLOGY 121, 317 (1993). (10.1083/jcb.121.2.317) / JOURNAL OF CELL BIOLOGY / RETRIEVAL OF TRANSMEMBRANE PROTEINS TO THE ENDOPLASMIC-RETICULUM by JACKSON M.R. (1993)
JAFFE L.A., DEV BIOL 156, 556 (1993). (10.1006/dbio.1993.1103) / DEV BIOL by JAFFE L.A. (1993)
KUISMANEN ELOW TEMPERATURE-INDUCED TRANSPORT BLOCKS AS TOOLS TO MANIPULATE MEMBRANE TRAFFICMETHODS IN CELL BIOLOGY 32, 257 (1989). (10.1016/S0091-679X(08)61174-7) / METHODS IN CELL BIOLOGY / LOW TEMPERATURE-INDUCED TRANSPORT BLOCKS AS TOOLS TO MANIPULATE MEMBRANE TRAFFIC by KUISMANEN E (1989)
LEWIS M.J.LIGAND-INDUCED REDISTRIBUTION OF A HUMAN KDEL RECEPTOR FROM THE GOLGI-COMPLEX TO THE ENDOPLASMIC-RETICULUMCELL 68, 353 (1992). (10.1016/0092-8674(92)90476-S) / CELL / LIGAND-INDUCED REDISTRIBUTION OF A HUMAN KDEL RECEPTOR FROM THE GOLGI-COMPLEX TO THE ENDOPLASMIC-RETICULUM by LEWIS M.J. (1992)
LIPPINCOTTSCHWA JMICROTUBULE-DEPENDENT RETROGRADE TRANSPORT OF PROTEINS INTO THE ER IN THE PRESENCE OF BREFELDIN-A SUGGESTS AN ER RECYCLING PATHWAYCELL 60, 821 (1990). (10.1016/0092-8674(90)90096-W) / CELL / MICROTUBULE-DEPENDENT RETROGRADE TRANSPORT OF PROTEINS INTO THE ER IN THE PRESENCE OF BREFELDIN-A SUGGESTS AN ER RECYCLING PATHWAY by LIPPINCOTTSCHWA J (1990)
LUZIO J.P.EUKARYOTIC MEMBRANE TRAFFIC - RETRIEVAL AND RETENTION MECHANISMS TO ACHIEVE ORGANELLE RESIDENCETRENDS IN BIOCHEMICAL SCIENCES 18, 395 (1993). (10.1016/0968-0004(93)90097-7) / TRENDS IN BIOCHEMICAL SCIENCES / EUKARYOTIC MEMBRANE TRAFFIC - RETRIEVAL AND RETENTION MECHANISMS TO ACHIEVE ORGANELLE RESIDENCE by LUZIO J.P. (1993)
MACHAMER C.E., CURR OPIN CELL BIOL 5, 606 (1993). (10.1016/0955-0674(93)90129-E) / CURR OPIN CELL BIOL by MACHAMER C.E. (1993)
MASRI K.A.IDENTIFICATION OF THE FULL-LENGTH CODING SEQUENCE FOR HUMAN GALACTOSYLTRANSFERASE (BETA-N-ACETYLGLUCOSAMINIDE - BETA-1,4-GALACTOSYLTRANSFERASE)BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 157, 657 (1988). (10.1016/S0006-291X(88)80300-0) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS / IDENTIFICATION OF THE FULL-LENGTH CODING SEQUENCE FOR HUMAN GALACTOSYLTRANSFERASE (BETA-N-ACETYLGLUCOSAMINIDE - BETA-1,4-GALACTOSYLTRANSFERASE) by MASRI K.A. (1988)
MELANCON PINVOLVEMENT OF GTP-BINDING G PROTEINS IN TRANSPORT THROUGH THE GOLGI STACKCELL 51, 1053 (1987). (10.1016/0092-8674(87)90591-5) / CELL / INVOLVEMENT OF GTP-BINDING G PROTEINS IN TRANSPORT THROUGH THE GOLGI STACK by MELANCON P (1987)
MELLMAN ITHE GOLGI-COMPLEX - INVITRO VERITASCELL 68, 829 (1992). (10.1016/0092-8674(92)90027-A) / CELL / THE GOLGI-COMPLEX - INVITRO VERITAS by MELLMAN I (1992)
MOREMEN K.W.ISOLATION, CHARACTERIZATION, AND EXPRESSION OF CDNAS ENCODING MURINE ALPHA-MANNOSIDASE-II, A GOLGI ENZYME THAT CONTROLS CONVERSION OF HIGH MANNOSE TO COMPLEX N-GLYCANSJOURNAL OF CELL BIOLOGY 115, 1521 (1991). (10.1083/jcb.115.6.1521) / JOURNAL OF CELL BIOLOGY / ISOLATION, CHARACTERIZATION, AND EXPRESSION OF CDNAS ENCODING MURINE ALPHA-MANNOSIDASE-II, A GOLGI ENZYME THAT CONTROLS CONVERSION OF HIGH MANNOSE TO COMPLEX N-GLYCANS by MOREMEN K.W. (1991)
MUNRO SAN INVESTIGATION OF THE ROLE OF TRANSMEMBRANE DOMAINS IN GOLGI PROTEIN RETENTIONEMBO JOURNAL 14, 4695 (1995). (10.1002/j.1460-2075.1995.tb00151.x) / EMBO JOURNAL / AN INVESTIGATION OF THE ROLE OF TRANSMEMBRANE DOMAINS IN GOLGI PROTEIN RETENTION by MUNRO S (1995)
NILSSON TRETENTION AND RETRIEVAL IN THE ENDOPLASMIC-RETICULUM AND THE GOLGI-APPARATUSCURRENT OPINION IN CELL BIOLOGY 6, 517 (1994). (10.1016/0955-0674(94)90070-1) / CURRENT OPINION IN CELL BIOLOGY / RETENTION AND RETRIEVAL IN THE ENDOPLASMIC-RETICULUM AND THE GOLGI-APPARATUS by NILSSON T (1994)
NILSSON TKIN RECOGNITION - A MODEL FOR THE RETENTION OF GOLGI ENZYMESFEBS LETTERS 330, 1 (1993). (10.1016/0014-5793(93)80906-B) / FEBS LETTERS / KIN RECOGNITION - A MODEL FOR THE RETENTION OF GOLGI ENZYMES by NILSSON T (1993)
PETERS P unpublished data.
PRASHER D.C., GENE 15, 111 (1992). / GENE by PRASHER D.C. (1992)
RAMBOURG A3-DIMENSIONAL ELECTRON-MICROSCOPY - STRUCTURE OF THE GOLGI-APPARATUSEUROPEAN JOURNAL OF CELL BIOLOGY 51, 189 (1990). / EUROPEAN JOURNAL OF CELL BIOLOGY / 3-DIMENSIONAL ELECTRON-MICROSCOPY - STRUCTURE OF THE GOLGI-APPARATUS by RAMBOURG A (1990)
ROTHMAN J.E.IMPLICATIONS OF THE SNARE HYPOTHESIS FOR INTRACELLULAR MEMBRANE TOPOLOGY AND DYNAMICSCURRENT BIOLOGY 4, 220 (1994). (10.1016/S0960-9822(00)00051-8) / CURRENT BIOLOGY / IMPLICATIONS OF THE SNARE HYPOTHESIS FOR INTRACELLULAR MEMBRANE TOPOLOGY AND DYNAMICS by ROTHMAN J.E. (1994)
ROTHMAN J.E.MOLECULAR DISSECTION OF THE SECRETORY PATHWAYNATURE 355, 409 (1992). (10.1038/355409a0) / NATURE / MOLECULAR DISSECTION OF THE SECRETORY PATHWAY by ROTHMAN J.E. (1992)
STORRIE BTHE INTRACELLULAR MOBILITY OF A VIRAL MEMBRANE GLYCOPROTEIN MEASURED BY CONFOCAL MICROSCOPE FLUORESCENCE RECOVERY AFTER PHOTOBLEACHINGJOURNAL OF CELL SCIENCE 107, 1309 (1994). (10.1242/jcs.107.5.1309) / JOURNAL OF CELL SCIENCE / THE INTRACELLULAR MOBILITY OF A VIRAL MEMBRANE GLYCOPROTEIN MEASURED BY CONFOCAL MICROSCOPE FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING by STORRIE B (1994)
TANAKA K3-DIMENSIONAL ARCHITECTURE OF THE GOLGI-COMPLEX OBSERVED BY HIGH-RESOLUTION SCANNING ELECTRON-MICROSCOPYJOURNAL OF SUBMICROSCOPIC CYTOLOGY AND PATHOLOGY 18, 1 (1986). / JOURNAL OF SUBMICROSCOPIC CYTOLOGY AND PATHOLOGY / 3-DIMENSIONAL ARCHITECTURE OF THE GOLGI-COMPLEX OBSERVED BY HIGH-RESOLUTION SCANNING ELECTRON-MICROSCOPY by TANAKA K (1986)
TOWNSLEY F.M.MUTATIONAL ANALYSIS OF THE HUMAN KDEL RECEPTOR - DISTINCT STRUCTURAL REQUIREMENTS FOR GOLGI RETENTION, LIGAND-BINDING AND RETROGRADE TRANSPORTEMBO JOURNAL 12, 2821 (1993). (10.1002/j.1460-2075.1993.tb05943.x) / EMBO JOURNAL / MUTATIONAL ANALYSIS OF THE HUMAN KDEL RECEPTOR - DISTINCT STRUCTURAL REQUIREMENTS FOR GOLGI RETENTION, LIGAND-BINDING AND RETROGRADE TRANSPORT by TOWNSLEY F.M. (1993)
WEIDMAN PGOLGI MEMBRANE DYNAMICS IMAGED BY FREEZE-ETCH ELECTRON-MICROSCOPY - VIEWS OF DIFFERENT MEMBRANE COATINGS INVOLVED IN TUBULATION VERSUS VESICULATIONCELL 75, 123 (1993). (10.1016/S0092-8674(05)80089-3) / CELL / GOLGI MEMBRANE DYNAMICS IMAGED BY FREEZE-ETCH ELECTRON-MICROSCOPY - VIEWS OF DIFFERENT MEMBRANE COATINGS INVOLVED IN TUBULATION VERSUS VESICULATION by WEIDMAN P (1993)
WEIDMAN P.J.ANTEROGRADE TRANSPORT THROUGH THE GOLGI-COMPLEX - DO GOLGI TUBULES HOLD THE KEYTRENDS IN CELL BIOLOGY 5, 302 (1995). (10.1016/S0962-8924(00)89046-7) / TRENDS IN CELL BIOLOGY / ANTEROGRADE TRANSPORT THROUGH THE GOLGI-COMPLEX - DO GOLGI TUBULES HOLD THE KEY by WEIDMAN P.J. (1995)
WEY C.L.LATERAL DIFFUSION OF RHODOPSIN IN PHOTORECEPTOR CELLS MEASURED BY FLUORESCENCE PHOTOBLEACHING AND RECOVERYBIOPHYSICAL JOURNAL 33, 225 (1981). (10.1016/S0006-3495(81)84883-7) / BIOPHYSICAL JOURNAL / LATERAL DIFFUSION OF RHODOPSIN IN PHOTORECEPTOR CELLS MEASURED BY FLUORESCENCE PHOTOBLEACHING AND RECOVERY by WEY C.L. (1981)
WIER M.L.EFFECTS OF CELL-DENSITY AND EXTRACELLULAR-MATRIX ON THE LATERAL DIFFUSION OF MAJOR HISTOCOMPATIBILITY ANTIGENS IN CULTURED FIBROBLASTSJOURNAL OF CELL BIOLOGY 103, 215 (1986). (10.1083/jcb.103.1.215) / JOURNAL OF CELL BIOLOGY / EFFECTS OF CELL-DENSITY AND EXTRACELLULAR-MATRIX ON THE LATERAL DIFFUSION OF MAJOR HISTOCOMPATIBILITY ANTIGENS IN CULTURED FIBROBLASTS by WIER M.L. (1986)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 10:30 a.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 6:14 p.m.)
Indexed	3 weeks ago (Aug. 7, 2025, 5 p.m.)
Issued	29 years ago (Aug. 9, 1996)
Published	29 years ago (Aug. 9, 1996)
Published Print	29 years ago (Aug. 9, 1996)

Funders 0

None

BibTeX

@article{Cole_1996, title={Diffusional Mobility of Golgi Proteins in Membranes of Living Cells}, volume={273}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.273.5276.797}, DOI={10.1126/science.273.5276.797}, number={5276}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Cole, Nelson B. and Smith, Carolyn L. and Sciaky, Noah and Terasaki, Mark and Edidin, Michael and Lippincott-Schwartz, Jennifer}, year={1996}, month=aug, pages={797–801} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        7
      ]
    ],
    "date-time": "2025-08-07T21:00:18Z",
    "timestamp": 1754600418938
  },
  "reference-count": 36,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "5276",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1996,
        8,
        9
      ]
    ]
  },
  "abstract": "<jats:p>\n            The mechanism by which Golgi membrane proteins are retained within the Golgi complex in the midst of a continuous flow of protein and lipid is not yet understood. The diffusional mobilities of mammalian Golgi membrane proteins fused with green fluorescent protein from\n            <jats:italic>Aequorea victoria</jats:italic>\n            were measured in living HeLa cells with the fluorescence photobleaching recovery technique. The diffusion coefficients ranged from 3 \u00d7 10\n            <jats:sup>\u22129</jats:sup>\n            square centimeters per second to 5 \u00d7 10\n            <jats:sup>\u22129</jats:sup>\n            square centimeters per second, with greater than 90 percent of the chimeric proteins mobile. Extensive lateral diffusion of the chimeric proteins occurred between Golgi stacks. Thus, the chimeras diffuse rapidly and freely in Golgi membranes, which suggests that Golgi targeting and retention of these molecules does not depend on protein immobilization.\n          </jats:p>",
  "DOI": "10.1126/science.273.5276.797",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        10,
        27
      ]
    ],
    "date-time": "2006-10-27T14:30:41Z",
    "timestamp": 1161959441000
  },
  "page": "797-801",
  "source": "Crossref",
  "is-referenced-by-count": 433,
  "title": "Diffusional Mobility of Golgi Proteins in Membranes of Living Cells",
  "prefix": "10.1126",
  "volume": "273",
  "author": [
    {
      "given": "Nelson B.",
      "family": "Cole",
      "sequence": "first",
      "affiliation": [
        {
          "name": "N. B. Cole, N. Sciaky, J. Lippincott-Schwartz, Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Building 18T, National Institutes of Health, Bethesda, MD 20892, USA."
        }
      ]
    },
    {
      "given": "Carolyn L.",
      "family": "Smith",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "C. L. Smith, National Institute of Neurological Disorders and Stroke, Building 36, National Institutes of Health, Bethesda, MD 20892, USA."
        }
      ]
    },
    {
      "given": "Noah",
      "family": "Sciaky",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "N. B. Cole, N. Sciaky, J. Lippincott-Schwartz, Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Building 18T, National Institutes of Health, Bethesda, MD 20892, USA."
        }
      ]
    },
    {
      "given": "Mark",
      "family": "Terasaki",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "M. Terasaki, Department of Physiology, University of Connecticut Health Center, Farmington, CT 06032, USA."
        }
      ]
    },
    {
      "given": "Michael",
      "family": "Edidin",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "M. Edidin, Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA."
        }
      ]
    },
    {
      "given": "Jennifer",
      "family": "Lippincott-Schwartz",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "N. B. Cole, N. Sciaky, J. Lippincott-Schwartz, Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Building 18T, National Institutes of Health, Bethesda, MD 20892, USA."
        }
      ]
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_3_1_2_2",
      "doi-asserted-by": "crossref",
      "first-page": "523",
      "DOI": "10.1016/0092-8674(87)90025-0",
      "article-title": "SEMI-INTACT CELLS PERMEABLE TO MACROMOLECULES - USE IN RECONSTITUTION OF PROTEIN-TRANSPORT FROM THE ENDOPLASMIC-RETICULUM TO THE GOLGI-COMPLEX",
      "volume": "50",
      "author": "BECKERS CJM",
      "year": "1987",
      "unstructured": "BECKERS CJMSEMI-INTACT CELLS PERMEABLE TO MACROMOLECULES - USE IN RECONSTITUTION OF PROTEIN-TRANSPORT FROM THE ENDOPLASMIC-RETICULUM TO THE GOLGI-COMPLEXCELL 50, 523 (1987).",
      "journal-title": "CELL"
    },
    {
      "key": "e_1_3_1_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.8362242"
    },
    {
      "key": "e_1_3_1_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.8303295"
    },
    {
      "key": "e_1_3_1_5_2",
      "doi-asserted-by": "crossref",
      "first-page": "135",
      "DOI": "10.1016/0092-8674(90)90221-Y",
      "article-title": "TUBULOVESICULAR PROCESSES EMERGE FROM TRANS-GOLGI CISTERNAE, EXTEND ALONG MICROTUBULES, AND INTERLINK ADJACENT TRANS-GOLGI ELEMENTS INTO A RETICULUM",
      "volume": "61",
      "author": "COOPER M.S.",
      "year": "1990",
      "unstructured": "COOPER M.S.TUBULOVESICULAR PROCESSES EMERGE FROM TRANS-GOLGI CISTERNAE, EXTEND ALONG MICROTUBULES, AND INTERLINK ADJACENT TRANS-GOLGI ELEMENTS INTO A RETICULUMCELL 61, 135 (1990).",
      "journal-title": "CELL"
    },
    {
      "key": "e_1_3_1_6_2",
      "doi-asserted-by": "crossref",
      "first-page": "2295",
      "DOI": "10.1083/jcb.111.6.2295",
      "article-title": "DISSOCIATION OF A 110-KD PERIPHERAL MEMBRANE-PROTEIN FROM THE GOLGI-APPARATUS IS AN EARLY EVENT IN BREFELDIN-A ACTION",
      "volume": "111",
      "author": "DONALDSON J.G.",
      "year": "1990",
      "unstructured": "DONALDSON J.G.DISSOCIATION OF A 110-KD PERIPHERAL MEMBRANE-PROTEIN FROM THE GOLGI-APPARATUS IS AN EARLY EVENT IN BREFELDIN-A ACTIONJOURNAL OF CELL BIOLOGY 111, 2295 (1990).",
      "journal-title": "JOURNAL OF CELL BIOLOGY"
    },
    {
      "key": "e_1_3_1_7_2",
      "doi-asserted-by": "crossref",
      "first-page": "579",
      "DOI": "10.1083/jcb.112.4.579",
      "article-title": "GUANINE-NUCLEOTIDES MODULATE THE EFFECTS OF BREFELDIN-A IN SEMIPERMEABLE CELLS - REGULATION OF THE ASSOCIATION OF A 110-KD PERIPHERAL MEMBRANE-PROTEIN WITH THE GOLGI-APPARATUS",
      "volume": "112",
      "author": "DONALDSON J.G.",
      "year": "1991",
      "unstructured": "DONALDSON J.G.GUANINE-NUCLEOTIDES MODULATE THE EFFECTS OF BREFELDIN-A IN SEMIPERMEABLE CELLS - REGULATION OF THE ASSOCIATION OF A 110-KD PERIPHERAL MEMBRANE-PROTEIN WITH THE GOLGI-APPARATUSJOURNAL OF CELL BIOLOGY 112, 579 (1991).",
      "journal-title": "JOURNAL OF CELL BIOLOGY"
    },
    {
      "key": "e_1_3_1_8_2",
      "first-page": "109",
      "author": "EDIDIN M",
      "year": "1994",
      "unstructured": "EDIDIN M, MOBILITY PROXIMITY B1994109.",
      "journal-title": "MOBILITY PROXIMITY B"
    },
    {
      "key": "e_1_3_1_9_2",
      "doi-asserted-by": "crossref",
      "first-page": "277",
      "DOI": "10.1083/jcb.108.2.277",
      "article-title": "THE DYNAMIC NATURE OF THE GOLGI-COMPLEX",
      "volume": "108",
      "author": "GRIFFITHS G",
      "year": "1989",
      "unstructured": "GRIFFITHS GTHE DYNAMIC NATURE OF THE GOLGI-COMPLEXJOURNAL OF CELL BIOLOGY 108, 277 (1989).",
      "journal-title": "JOURNAL OF CELL BIOLOGY"
    },
    {
      "key": "e_1_3_1_10_2",
      "doi-asserted-by": "crossref",
      "first-page": "663",
      "DOI": "10.1038/373663b0",
      "article-title": "IMPROVED GREEN FLUORESCENCE",
      "volume": "373",
      "author": "HEIM R",
      "year": "1995",
      "unstructured": "HEIM RIMPROVED GREEN FLUORESCENCENATURE 373, 663 (1995).",
      "journal-title": "NATURE"
    },
    {
      "key": "e_1_3_1_11_2",
      "doi-asserted-by": "crossref",
      "first-page": "625",
      "DOI": "10.1016/0092-8674(92)90226-3",
      "article-title": "A BREFELDIN-A-LIKE PHENOTYPE IS INDUCED BY THE OVEREXPRESSION OF A HUMAN ERD-2-LIKE PROTEIN, ELP-1",
      "volume": "69",
      "author": "HSU V.W.",
      "year": "1992",
      "unstructured": "HSU V.W.A BREFELDIN-A-LIKE PHENOTYPE IS INDUCED BY THE OVEREXPRESSION OF A HUMAN ERD-2-LIKE PROTEIN, ELP-1CELL 69, 625 (1992).",
      "journal-title": "CELL"
    },
    {
      "key": "e_1_3_1_12_2",
      "doi-asserted-by": "crossref",
      "first-page": "317",
      "DOI": "10.1083/jcb.121.2.317",
      "article-title": "RETRIEVAL OF TRANSMEMBRANE PROTEINS TO THE ENDOPLASMIC-RETICULUM",
      "volume": "121",
      "author": "JACKSON M.R.",
      "year": "1993",
      "unstructured": "JACKSON M.R.RETRIEVAL OF TRANSMEMBRANE PROTEINS TO THE ENDOPLASMIC-RETICULUMJOURNAL OF CELL BIOLOGY 121, 317 (1993).",
      "journal-title": "JOURNAL OF CELL BIOLOGY"
    },
    {
      "key": "e_1_3_1_13_2",
      "doi-asserted-by": "crossref",
      "first-page": "556",
      "DOI": "10.1006/dbio.1993.1103",
      "volume": "156",
      "author": "JAFFE L.A.",
      "year": "1993",
      "unstructured": "JAFFE L.A., DEV BIOL 156, 556 (1993).",
      "journal-title": "DEV BIOL"
    },
    {
      "key": "e_1_3_1_14_2",
      "doi-asserted-by": "crossref",
      "first-page": "257",
      "DOI": "10.1016/S0091-679X(08)61174-7",
      "article-title": "LOW TEMPERATURE-INDUCED TRANSPORT BLOCKS AS TOOLS TO MANIPULATE MEMBRANE TRAFFIC",
      "volume": "32",
      "author": "KUISMANEN E",
      "year": "1989",
      "unstructured": "KUISMANEN ELOW TEMPERATURE-INDUCED TRANSPORT BLOCKS AS TOOLS TO MANIPULATE MEMBRANE TRAFFICMETHODS IN CELL BIOLOGY 32, 257 (1989).",
      "journal-title": "METHODS IN CELL BIOLOGY"
    },
    {
      "key": "e_1_3_1_15_2",
      "doi-asserted-by": "crossref",
      "first-page": "353",
      "DOI": "10.1016/0092-8674(92)90476-S",
      "article-title": "LIGAND-INDUCED REDISTRIBUTION OF A HUMAN KDEL RECEPTOR FROM THE GOLGI-COMPLEX TO THE ENDOPLASMIC-RETICULUM",
      "volume": "68",
      "author": "LEWIS M.J.",
      "year": "1992",
      "unstructured": "LEWIS M.J.LIGAND-INDUCED REDISTRIBUTION OF A HUMAN KDEL RECEPTOR FROM THE GOLGI-COMPLEX TO THE ENDOPLASMIC-RETICULUMCELL 68, 353 (1992).",
      "journal-title": "CELL"
    },
    {
      "key": "e_1_3_1_16_2",
      "doi-asserted-by": "crossref",
      "first-page": "821",
      "DOI": "10.1016/0092-8674(90)90096-W",
      "article-title": "MICROTUBULE-DEPENDENT RETROGRADE TRANSPORT OF PROTEINS INTO THE ER IN THE PRESENCE OF BREFELDIN-A SUGGESTS AN ER RECYCLING PATHWAY",
      "volume": "60",
      "author": "LIPPINCOTTSCHWA J",
      "year": "1990",
      "unstructured": "LIPPINCOTTSCHWA JMICROTUBULE-DEPENDENT RETROGRADE TRANSPORT OF PROTEINS INTO THE ER IN THE PRESENCE OF BREFELDIN-A SUGGESTS AN ER RECYCLING PATHWAYCELL 60, 821 (1990).",
      "journal-title": "CELL"
    },
    {
      "key": "e_1_3_1_17_2",
      "doi-asserted-by": "crossref",
      "first-page": "395",
      "DOI": "10.1016/0968-0004(93)90097-7",
      "article-title": "EUKARYOTIC MEMBRANE TRAFFIC - RETRIEVAL AND RETENTION MECHANISMS TO ACHIEVE ORGANELLE RESIDENCE",
      "volume": "18",
      "author": "LUZIO J.P.",
      "year": "1993",
      "unstructured": "LUZIO J.P.EUKARYOTIC MEMBRANE TRAFFIC - RETRIEVAL AND RETENTION MECHANISMS TO ACHIEVE ORGANELLE RESIDENCETRENDS IN BIOCHEMICAL SCIENCES 18, 395 (1993).",
      "journal-title": "TRENDS IN BIOCHEMICAL SCIENCES"
    },
    {
      "key": "e_1_3_1_18_2",
      "doi-asserted-by": "crossref",
      "first-page": "606",
      "DOI": "10.1016/0955-0674(93)90129-E",
      "volume": "5",
      "author": "MACHAMER C.E.",
      "year": "1993",
      "unstructured": "MACHAMER C.E., CURR OPIN CELL BIOL 5, 606 (1993).",
      "journal-title": "CURR OPIN CELL BIOL"
    },
    {
      "key": "e_1_3_1_19_2",
      "doi-asserted-by": "crossref",
      "first-page": "657",
      "DOI": "10.1016/S0006-291X(88)80300-0",
      "article-title": "IDENTIFICATION OF THE FULL-LENGTH CODING SEQUENCE FOR HUMAN GALACTOSYLTRANSFERASE (BETA-N-ACETYLGLUCOSAMINIDE - BETA-1,4-GALACTOSYLTRANSFERASE)",
      "volume": "157",
      "author": "MASRI K.A.",
      "year": "1988",
      "unstructured": "MASRI K.A.IDENTIFICATION OF THE FULL-LENGTH CODING SEQUENCE FOR HUMAN GALACTOSYLTRANSFERASE (BETA-N-ACETYLGLUCOSAMINIDE - BETA-1,4-GALACTOSYLTRANSFERASE)BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 157, 657 (1988).",
      "journal-title": "BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS"
    },
    {
      "key": "e_1_3_1_20_2",
      "doi-asserted-by": "crossref",
      "first-page": "1053",
      "DOI": "10.1016/0092-8674(87)90591-5",
      "article-title": "INVOLVEMENT OF GTP-BINDING G PROTEINS IN TRANSPORT THROUGH THE GOLGI STACK",
      "volume": "51",
      "author": "MELANCON P",
      "year": "1987",
      "unstructured": "MELANCON PINVOLVEMENT OF GTP-BINDING G PROTEINS IN TRANSPORT THROUGH THE GOLGI STACKCELL 51, 1053 (1987).",
      "journal-title": "CELL"
    },
    {
      "key": "e_1_3_1_21_2",
      "doi-asserted-by": "crossref",
      "first-page": "829",
      "DOI": "10.1016/0092-8674(92)90027-A",
      "article-title": "THE GOLGI-COMPLEX - INVITRO VERITAS",
      "volume": "68",
      "author": "MELLMAN I",
      "year": "1992",
      "unstructured": "MELLMAN ITHE GOLGI-COMPLEX - INVITRO VERITASCELL 68, 829 (1992).",
      "journal-title": "CELL"
    },
    {
      "key": "e_1_3_1_22_2",
      "doi-asserted-by": "crossref",
      "first-page": "1521",
      "DOI": "10.1083/jcb.115.6.1521",
      "article-title": "ISOLATION, CHARACTERIZATION, AND EXPRESSION OF CDNAS ENCODING MURINE ALPHA-MANNOSIDASE-II, A GOLGI ENZYME THAT CONTROLS CONVERSION OF HIGH MANNOSE TO COMPLEX N-GLYCANS",
      "volume": "115",
      "author": "MOREMEN K.W.",
      "year": "1991",
      "unstructured": "MOREMEN K.W.ISOLATION, CHARACTERIZATION, AND EXPRESSION OF CDNAS ENCODING MURINE ALPHA-MANNOSIDASE-II, A GOLGI ENZYME THAT CONTROLS CONVERSION OF HIGH MANNOSE TO COMPLEX N-GLYCANSJOURNAL OF CELL BIOLOGY 115, 1521 (1991).",
      "journal-title": "JOURNAL OF CELL BIOLOGY"
    },
    {
      "key": "e_1_3_1_23_2",
      "doi-asserted-by": "crossref",
      "first-page": "4695",
      "DOI": "10.1002/j.1460-2075.1995.tb00151.x",
      "article-title": "AN INVESTIGATION OF THE ROLE OF TRANSMEMBRANE DOMAINS IN GOLGI PROTEIN RETENTION",
      "volume": "14",
      "author": "MUNRO S",
      "year": "1995",
      "unstructured": "MUNRO SAN INVESTIGATION OF THE ROLE OF TRANSMEMBRANE DOMAINS IN GOLGI PROTEIN RETENTIONEMBO JOURNAL 14, 4695 (1995).",
      "journal-title": "EMBO JOURNAL"
    },
    {
      "key": "e_1_3_1_24_2",
      "doi-asserted-by": "crossref",
      "first-page": "517",
      "DOI": "10.1016/0955-0674(94)90070-1",
      "article-title": "RETENTION AND RETRIEVAL IN THE ENDOPLASMIC-RETICULUM AND THE GOLGI-APPARATUS",
      "volume": "6",
      "author": "NILSSON T",
      "year": "1994",
      "unstructured": "NILSSON TRETENTION AND RETRIEVAL IN THE ENDOPLASMIC-RETICULUM AND THE GOLGI-APPARATUSCURRENT OPINION IN CELL BIOLOGY 6, 517 (1994).",
      "journal-title": "CURRENT OPINION IN CELL BIOLOGY"
    },
    {
      "key": "e_1_3_1_25_2",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1016/0014-5793(93)80906-B",
      "article-title": "KIN RECOGNITION - A MODEL FOR THE RETENTION OF GOLGI ENZYMES",
      "volume": "330",
      "author": "NILSSON T",
      "year": "1993",
      "unstructured": "NILSSON TKIN RECOGNITION - A MODEL FOR THE RETENTION OF GOLGI ENZYMESFEBS LETTERS 330, 1 (1993).",
      "journal-title": "FEBS LETTERS"
    },
    {
      "key": "e_1_3_1_26_2",
      "unstructured": "PETERS P unpublished data."
    },
    {
      "key": "e_1_3_1_27_2",
      "first-page": "111",
      "volume": "15",
      "author": "PRASHER D.C.",
      "year": "1992",
      "unstructured": "PRASHER D.C., GENE 15, 111 (1992).",
      "journal-title": "GENE"
    },
    {
      "key": "e_1_3_1_28_2",
      "first-page": "189",
      "article-title": "3-DIMENSIONAL ELECTRON-MICROSCOPY - STRUCTURE OF THE GOLGI-APPARATUS",
      "volume": "51",
      "author": "RAMBOURG A",
      "year": "1990",
      "unstructured": "RAMBOURG A3-DIMENSIONAL ELECTRON-MICROSCOPY - STRUCTURE OF THE GOLGI-APPARATUSEUROPEAN JOURNAL OF CELL BIOLOGY 51, 189 (1990).",
      "journal-title": "EUROPEAN JOURNAL OF CELL BIOLOGY"
    },
    {
      "key": "e_1_3_1_29_2",
      "doi-asserted-by": "crossref",
      "first-page": "220",
      "DOI": "10.1016/S0960-9822(00)00051-8",
      "article-title": "IMPLICATIONS OF THE SNARE HYPOTHESIS FOR INTRACELLULAR MEMBRANE TOPOLOGY AND DYNAMICS",
      "volume": "4",
      "author": "ROTHMAN J.E.",
      "year": "1994",
      "unstructured": "ROTHMAN J.E.IMPLICATIONS OF THE SNARE HYPOTHESIS FOR INTRACELLULAR MEMBRANE TOPOLOGY AND DYNAMICSCURRENT BIOLOGY 4, 220 (1994).",
      "journal-title": "CURRENT BIOLOGY"
    },
    {
      "key": "e_1_3_1_30_2",
      "doi-asserted-by": "crossref",
      "first-page": "409",
      "DOI": "10.1038/355409a0",
      "article-title": "MOLECULAR DISSECTION OF THE SECRETORY PATHWAY",
      "volume": "355",
      "author": "ROTHMAN J.E.",
      "year": "1992",
      "unstructured": "ROTHMAN J.E.MOLECULAR DISSECTION OF THE SECRETORY PATHWAYNATURE 355, 409 (1992).",
      "journal-title": "NATURE"
    },
    {
      "key": "e_1_3_1_31_2",
      "doi-asserted-by": "crossref",
      "first-page": "1309",
      "DOI": "10.1242/jcs.107.5.1309",
      "article-title": "THE INTRACELLULAR MOBILITY OF A VIRAL MEMBRANE GLYCOPROTEIN MEASURED BY CONFOCAL MICROSCOPE FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING",
      "volume": "107",
      "author": "STORRIE B",
      "year": "1994",
      "unstructured": "STORRIE BTHE INTRACELLULAR MOBILITY OF A VIRAL MEMBRANE GLYCOPROTEIN MEASURED BY CONFOCAL MICROSCOPE FLUORESCENCE RECOVERY AFTER PHOTOBLEACHINGJOURNAL OF CELL SCIENCE 107, 1309 (1994).",
      "journal-title": "JOURNAL OF CELL SCIENCE"
    },
    {
      "key": "e_1_3_1_32_2",
      "first-page": "1",
      "article-title": "3-DIMENSIONAL ARCHITECTURE OF THE GOLGI-COMPLEX OBSERVED BY HIGH-RESOLUTION SCANNING ELECTRON-MICROSCOPY",
      "volume": "18",
      "author": "TANAKA K",
      "year": "1986",
      "unstructured": "TANAKA K3-DIMENSIONAL ARCHITECTURE OF THE GOLGI-COMPLEX OBSERVED BY HIGH-RESOLUTION SCANNING ELECTRON-MICROSCOPYJOURNAL OF SUBMICROSCOPIC CYTOLOGY AND PATHOLOGY 18, 1 (1986).",
      "journal-title": "JOURNAL OF SUBMICROSCOPIC CYTOLOGY AND PATHOLOGY"
    },
    {
      "key": "e_1_3_1_33_2",
      "doi-asserted-by": "crossref",
      "first-page": "2821",
      "DOI": "10.1002/j.1460-2075.1993.tb05943.x",
      "article-title": "MUTATIONAL ANALYSIS OF THE HUMAN KDEL RECEPTOR - DISTINCT STRUCTURAL REQUIREMENTS FOR GOLGI RETENTION, LIGAND-BINDING AND RETROGRADE TRANSPORT",
      "volume": "12",
      "author": "TOWNSLEY F.M.",
      "year": "1993",
      "unstructured": "TOWNSLEY F.M.MUTATIONAL ANALYSIS OF THE HUMAN KDEL RECEPTOR - DISTINCT STRUCTURAL REQUIREMENTS FOR GOLGI RETENTION, LIGAND-BINDING AND RETROGRADE TRANSPORTEMBO JOURNAL 12, 2821 (1993).",
      "journal-title": "EMBO JOURNAL"
    },
    {
      "key": "e_1_3_1_34_2",
      "doi-asserted-by": "crossref",
      "first-page": "123",
      "DOI": "10.1016/S0092-8674(05)80089-3",
      "article-title": "GOLGI MEMBRANE DYNAMICS IMAGED BY FREEZE-ETCH ELECTRON-MICROSCOPY - VIEWS OF DIFFERENT MEMBRANE COATINGS INVOLVED IN TUBULATION VERSUS VESICULATION",
      "volume": "75",
      "author": "WEIDMAN P",
      "year": "1993",
      "unstructured": "WEIDMAN PGOLGI MEMBRANE DYNAMICS IMAGED BY FREEZE-ETCH ELECTRON-MICROSCOPY - VIEWS OF DIFFERENT MEMBRANE COATINGS INVOLVED IN TUBULATION VERSUS VESICULATIONCELL 75, 123 (1993).",
      "journal-title": "CELL"
    },
    {
      "key": "e_1_3_1_35_2",
      "doi-asserted-by": "crossref",
      "first-page": "302",
      "DOI": "10.1016/S0962-8924(00)89046-7",
      "article-title": "ANTEROGRADE TRANSPORT THROUGH THE GOLGI-COMPLEX - DO GOLGI TUBULES HOLD THE KEY",
      "volume": "5",
      "author": "WEIDMAN P.J.",
      "year": "1995",
      "unstructured": "WEIDMAN P.J.ANTEROGRADE TRANSPORT THROUGH THE GOLGI-COMPLEX - DO GOLGI TUBULES HOLD THE KEYTRENDS IN CELL BIOLOGY 5, 302 (1995).",
      "journal-title": "TRENDS IN CELL BIOLOGY"
    },
    {
      "key": "e_1_3_1_36_2",
      "doi-asserted-by": "crossref",
      "first-page": "225",
      "DOI": "10.1016/S0006-3495(81)84883-7",
      "article-title": "LATERAL DIFFUSION OF RHODOPSIN IN PHOTORECEPTOR CELLS MEASURED BY FLUORESCENCE PHOTOBLEACHING AND RECOVERY",
      "volume": "33",
      "author": "WEY C.L.",
      "year": "1981",
      "unstructured": "WEY C.L.LATERAL DIFFUSION OF RHODOPSIN IN PHOTORECEPTOR CELLS MEASURED BY FLUORESCENCE PHOTOBLEACHING AND RECOVERYBIOPHYSICAL JOURNAL 33, 225 (1981).",
      "journal-title": "BIOPHYSICAL JOURNAL"
    },
    {
      "key": "e_1_3_1_37_2",
      "doi-asserted-by": "crossref",
      "first-page": "215",
      "DOI": "10.1083/jcb.103.1.215",
      "article-title": "EFFECTS OF CELL-DENSITY AND EXTRACELLULAR-MATRIX ON THE LATERAL DIFFUSION OF MAJOR HISTOCOMPATIBILITY ANTIGENS IN CULTURED FIBROBLASTS",
      "volume": "103",
      "author": "WIER M.L.",
      "year": "1986",
      "unstructured": "WIER M.L.EFFECTS OF CELL-DENSITY AND EXTRACELLULAR-MATRIX ON THE LATERAL DIFFUSION OF MAJOR HISTOCOMPATIBILITY ANTIGENS IN CULTURED FIBROBLASTSJOURNAL OF CELL BIOLOGY 103, 215 (1986).",
      "journal-title": "JOURNAL OF CELL BIOLOGY"
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.273.5276.797",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        12
      ]
    ],
    "date-time": "2024-01-12T23:14:33Z",
    "timestamp": 1705101273000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.273.5276.797"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1996,
        8,
        9
      ]
    ]
  },
  "references-count": 36,
  "journal-issue": {
    "issue": "5276",
    "published-print": {
      "date-parts": [
        [
          1996,
          8,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.273.5276.797"
  ],
  "URL": "http://dx.doi.org/10.1126/science.273.5276.797",
  "relation": {},
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1996,
        8,
        9
      ]
    ]
  }
}