DOI: 10.1126/science.272.5268.1646. Thymine-Thymine Dimer Bypass by Yeast DNA Polymerase ζ

Thymine-Thymine Dimer Bypass by Yeast DNA Polymerase ζ

10.1126/science.272.5268.1646

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The REV3 and REV7 genes of the yeast Saccharomyces cerevisiae are required for DNA damage-induced mutagenesis. The Rev3 and Rev7 proteins were shown to form a complex with DNA polymerase activity. This polymerase replicated past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally severely inhibits replication, with an efficiency of ∼10 percent. In contrast, bypass replication efficiency with yeast DNA polymerase α was no more than 1 percent. The Rev3-Rev7 complex is the sixth eukaryotic DNA polymerase to be described, and is therefore called DNA polymerase ζ.

Bibliography

Nelson, J. R., Lawrence, C. W., & Hinkle, D. C. (1996). Thymine-Thymine Dimer Bypass by Yeast DNA Polymerase Î¶. Science, 272(5268), 1646â1649.

Authors 3

John R. Nelson (first)
Christopher W. Lawrence (additional)
David C. Hinkle (additional)

References 17 Referenced 510

CHAN G.L., CYCLOBUTANE PYRIMIDINE DIMERS AND (6-4) PHOTOPRODUCTS BLOCK POLYMERIZATION BY DNA-POLYMERASE-I, BIOCHEMISTRY 24, 5723 (1985). (10.1021/bi00342a006) / BIOCHEMISTRY / CYCLOBUTANE PYRIMIDINE DIMERS AND (6-4) PHOTOPRODUCTS BLOCK POLYMERIZATION BY DNA-POLYMERASE-I by CHAN G.L. (1985)
FIELDS S, A NOVEL GENETIC SYSTEM TO DETECT PROTEIN PROTEIN INTERACTIONS, NATURE 340, 245 (1989). (10.1038/340245a0) / NATURE / A NOVEL GENETIC SYSTEM TO DETECT PROTEIN PROTEIN INTERACTIONS by FIELDS S (1989)
FRIEDBERG E.C., DNA REPAIR (1995). / DNA REPAIR by FRIEDBERG E.C. (1995)
GEITZ R.D., GENE 74, 527 (1988). (10.1016/0378-1119(88)90185-0) / GENE by GEITZ R.D. (1988)
HOLLENBERG S.M., IDENTIFICATION OF A NEW FAMILY OF TISSUE-SPECIFIC BASIC HELIX-LOOP-HELIX PROTEINS WITH A 2-HYBRID SYSTEM, MOLECULAR AND CELLULAR BIOLOGY 15, 3813 (1995). (10.1128/MCB.15.7.3813) / MOLECULAR AND CELLULAR BIOLOGY / IDENTIFICATION OF A NEW FAMILY OF TISSUE-SPECIFIC BASIC HELIX-LOOP-HELIX PROTEINS WITH A 2-HYBRID SYSTEM by HOLLENBERG S.M. (1995)
HOVLAND P, GALACTOSE AS A GRATUITOUS INDUCED OF GAL GENE-EXPRESSION IN YEASTS GROWING ON GLUCOSE, GENE 83, 57 (1989). (10.1016/0378-1119(89)90403-4) / GENE / GALACTOSE AS A GRATUITOUS INDUCED OF GAL GENE-EXPRESSION IN YEASTS GROWING ON GLUCOSE by HOVLAND P (1989)
LARIMER F.W., THE REV1 GENE OF SACCHAROMYCES-CEREVISIAE - ISOLATION, SEQUENCE, AND FUNCTIONAL-ANALYSIS, JOURNAL OF BACTERIOLOGY 171, 230 (1989). (10.1128/jb.171.1.230-237.1989) / JOURNAL OF BACTERIOLOGY / THE REV1 GENE OF SACCHAROMYCES-CEREVISIAE - ISOLATION, SEQUENCE, AND FUNCTIONAL-ANALYSIS by LARIMER F.W. (1989)
LARSON K.L., INFLUENCE OF TEMPLATE STRANDEDNESS ON INVITRO REPLICATION OF MUTAGEN-DAMAGED DNA, BIOCHEMISTRY 26, 2471 (1987). (10.1021/bi00383a011) / BIOCHEMISTRY / INFLUENCE OF TEMPLATE STRANDEDNESS ON INVITRO REPLICATION OF MUTAGEN-DAMAGED DNA by LARSON K.L. (1987)
MORRISON A, REV3, A SACCHAROMYCES-CEREVISIAE GENE WHOSE FUNCTION IS REQUIRED FOR INDUCED MUTAGENESIS, IS PREDICTED TO ENCODE A NONESSENTIAL DNA-POLYMERASE, JOURNAL OF BACTERIOLOGY 171, 5659 (1989). (10.1128/jb.171.10.5659-5667.1989) / JOURNAL OF BACTERIOLOGY / REV3, A SACCHAROMYCES-CEREVISIAE GENE WHOSE FUNCTION IS REQUIRED FOR INDUCED MUTAGENESIS, IS PREDICTED TO ENCODE A NONESSENTIAL DNA-POLYMERASE by MORRISON A (1989)
NELSON J.R. unpublished data.
ODAY C.L., PCNA-INDUCED DNA-SYNTHESIS PAST CIS-SYN AND TRANS-SYN-I THYMINE DIMERS BY CALF THYMUS DNA-POLYMERASE DELTA INVITRO, NUCLEIC ACIDS RESEARCH 20, 5403 (1992). (10.1093/nar/20.20.5403) / NUCLEIC ACIDS RESEARCH / PCNA-INDUCED DNA-SYNTHESIS PAST CIS-SYN AND TRANS-SYN-I THYMINE DIMERS BY CALF THYMUS DNA-POLYMERASE DELTA INVITRO by ODAY C.L. (1992)
PARK H.O., BUD2 ENCODES A GTPASE-ACTIVATING PROTEIN FOR BUD1/RSR1 NECESSARY FOR PROPER BUD-SITE SELECTION IN YEAST, NATURE 365, 269 (1993). (10.1038/365269a0) / NATURE / BUD2 ENCODES A GTPASE-ACTIVATING PROTEIN FOR BUD1/RSR1 NECESSARY FOR PROPER BUD-SITE SELECTION IN YEAST by PARK H.O. (1993)
RABKIN S.D., INVITRO BYPASS OF UV-INDUCED LESIONS BY ESCHERICHIA-COLI DNA-POLYMERASE-I - SPECIFICITY OF NUCLEOTIDE INCORPORATION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 80, 1541 (1983). (10.1073/pnas.80.6.1541) / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES / INVITRO BYPASS OF UV-INDUCED LESIONS BY ESCHERICHIA-COLI DNA-POLYMERASE-I - SPECIFICITY OF NUCLEOTIDE INCORPORATION by RABKIN S.D. (1983)
RAJAGOPALAN M, ACTIVITY OF THE PURIFIED MUTAGENESIS PROTEINS UMUC, UMUD', AND RECA IN REPLICATIVE BYPASS OF AN ABASIC DNA LESION BY DNA POLYMERASE-III, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 89, 10777 (1992). (10.1073/pnas.89.22.10777) / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA / ACTIVITY OF THE PURIFIED MUTAGENESIS PROTEINS UMUC, UMUD', AND RECA IN REPLICATIVE BYPASS OF AN ABASIC DNA LESION BY DNA POLYMERASE-III by RAJAGOPALAN M (1992)
SHIMIZU K, PURIFICATION AND CHARACTERIZATION OF A NEW DNA-POLYMERASE FROM BUDDING YEAST SACCHAROMYCES-CEREVISIAE - A PROBABLE HOMOLOG OF MAMMALIAN DNA-POLYMERASE BETA, JOURNAL OF BIOLOGICAL CHEMISTRY 268, 27148 (1993). (10.1016/S0021-9258(19)74229-X) / JOURNAL OF BIOLOGICAL CHEMISTRY / PURIFICATION AND CHARACTERIZATION OF A NEW DNA-POLYMERASE FROM BUDDING YEAST SACCHAROMYCES-CEREVISIAE - A PROBABLE HOMOLOG OF MAMMALIAN DNA-POLYMERASE BETA by SHIMIZU K (1993)
TAYLOR J.S., CIS-SYN THYMINE DIMERS ARE NOT ABSOLUTE BLOCKS TO REPLICATION BY DNA-POLYMERASE-I OF ESCHERICHIA-COLI INVITRO, BIOCHEMISTRY 29, 1624 (1990). (10.1021/bi00458a038) / BIOCHEMISTRY / CIS-SYN THYMINE DIMERS ARE NOT ABSOLUTE BLOCKS TO REPLICATION BY DNA-POLYMERASE-I OF ESCHERICHIA-COLI INVITRO by TAYLOR J.S. (1990)
TORPEY L.E., CLONING AND SEQUENCE OF REV7, A GENE WHOSE FUNCTION IS REQUIRED FOR DNA DAMAGE-INDUCED MUTAGENESIS IN SACCHAROMYCES-CEREVISIAE, YEAST 10, 1503 (1994). (10.1002/yea.320101115) / YEAST / CLONING AND SEQUENCE OF REV7, A GENE WHOSE FUNCTION IS REQUIRED FOR DNA DAMAGE-INDUCED MUTAGENESIS IN SACCHAROMYCES-CEREVISIAE by TORPEY L.E. (1994)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 2:30 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 9:47 p.m.)
Indexed	4 weeks ago (Aug. 3, 2025, 7:01 p.m.)
Issued	29 years, 2 months ago (June 14, 1996)
Published	29 years, 2 months ago (June 14, 1996)
Published Print	29 years, 2 months ago (June 14, 1996)

Funders 0

None

BibTeX

@article{Nelson_1996, title={Thymine-Thymine Dimer Bypass by Yeast DNA Polymerase ζ}, volume={272}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.272.5268.1646}, DOI={10.1126/science.272.5268.1646}, number={5268}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Nelson, John R. and Lawrence, Christopher W. and Hinkle, David C.}, year={1996}, month=jun, pages={1646–1649} }

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  }
}