Crystal Structure of the Lactose Operon Repressor and Its Complexes with DNA and Inducer
10.1126/science.271.5253.1247
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

The lac operon of Escherichia coli is the paradigm for gene regulation. Its key component is the lac repressor, a product of the lacI gene. The three-dimensional structures of the intact lac repressor, the lac repressor bound to the gratuitous inducer isopropyl-β-D-1-thiogalactoside (IPTG) and the lac repressor complexed with a 21-base pair symmetric operator DNA have been determined. These three structures show the conformation of the molecule in both the induced and repressed states and provide a framework for understanding a wealth of biochemical and genetic information. The DNA sequence of the lac operon has three lac repressor recognition sites in a stretch of 500 base pairs. The crystallographic structure of the complex with DNA suggests that the tetrameric repressor functions synergistically with catabolite gene activator protein (CAP) and participates in the quaternary formation of repression loops in which one tetrameric repressor interacts simultaneously with two sites on the genomic DNA.

Bibliography

Lewis, M., Chang, G., Horton, N. C., Kercher, M. A., Pace, H. C., Schumacher, M. A., Brennan, R. G., & Lu, P. (1996). Crystal Structure of the Lactose Operon Repressor and Its Complexes with DNA and Inducer. Science, 271(5253), 1247–1254.

Authors 8 University of Pennsylvania
  1. Mitchell Lewis (first) University of Pennsylvania
  2. Geoffrey Chang (additional) University of Pennsylvania
  3. Nancy C. Horton (additional) University of Pennsylvania
  4. Michele A. Kercher (additional) University of Pennsylvania
  5. Helen C. Pace (additional) University of Pennsylvania
  6. Maria A. Schumacher (additional)
  7. Richard G. Brennan (additional)
  8. Ponzy Lu (additional) University of Pennsylvania
References 75 Referenced 643
  1. ADHYA S, ANNU REV GENET 23, 207 (1989). (10.1146/annurev.ge.23.120189.001303) / ANNU REV GENET by ADHYA S (1989)
  2. ALBERTI S, DIMER-TO-TETRAMER ASSEMBLY OF LAC REPRESSOR INVOLVES A LEUCINE HEPTAD REPEAT, NEW BIOLOGIST 3, 57 (1991). / NEW BIOLOGIST / DIMER-TO-TETRAMER ASSEMBLY OF LAC REPRESSOR INVOLVES A LEUCINE HEPTAD REPEAT by ALBERTI S (1991)
  3. BELLOMY R. G., PHYSICAL-PROPERTIES OF DNA INVIVO AS PROBED BY THE LENGTH DEPENDENCE OF THE LAC OPERATOR LOOPING PROCESS, BIOCHEMISTRY 27, 3900 (1988). (10.1021/bi00411a002) / BIOCHEMISTRY / PHYSICAL-PROPERTIES OF DNA INVIVO AS PROBED BY THE LENGTH DEPENDENCE OF THE LAC OPERATOR LOOPING PROCESS by BELLOMY R. G. (1988)
  4. BETZ L. J., CLONING AND CHARACTERIZATION OF SEVERAL DOMINANT-NEGATIVE AND TIGHT-BINDING MUTANTS OF LAC REPRESSOR, GENE 42, 283 (1986). (10.1016/0378-1119(86)90232-5) / GENE / CLONING AND CHARACTERIZATION OF SEVERAL DOMINANT-NEGATIVE AND TIGHT-BINDING MUTANTS OF LAC REPRESSOR by BETZ L. J. (1986)
  5. BEYREUTHER K, AMINO-ACID SEQUENCE OF LAC REPRESSOR, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 70, 3576 (1973). (10.1073/pnas.70.12.3576) / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA / AMINO-ACID SEQUENCE OF LAC REPRESSOR by BEYREUTHER K (1973)
  6. BOURGEOIS S, ADV PROTEIN CHEM 30, 1 (1976). (10.1016/S0065-3233(08)60478-7) / ADV PROTEIN CHEM by BOURGEOIS S (1976)
  7. BRENOWITZ M, STABILITY OF A LAC REPRESSOR MEDIATED LOOPED COMPLEX, BIOCHEMISTRY 30, 5986 (1991). (10.1021/bi00238a024) / BIOCHEMISTRY / STABILITY OF A LAC REPRESSOR MEDIATED LOOPED COMPLEX by BRENOWITZ M (1991)
  8. BRENOWITZ M, J BIOL CHEM 286, 1281 (1991). (10.1016/S0021-9258(17)35313-9) / J BIOL CHEM by BRENOWITZ M (1991)
  9. 10.1126/science.235.4787.458
  10. BURSTEIN C, ROLE DU LACTOSE ET DE SES PRODUITS METABOLIQUES DANS LINDUCTION DE LOPERON LACTOSE CHEZ ESCHERICHIA COLI, BIOCHIMICA ET BIOPHYSICA ACTA 95, 634 (1965). (10.1016/0005-2787(65)90517-4) / BIOCHIMICA ET BIOPHYSICA ACTA / ROLE DU LACTOSE ET DE SES PRODUITS METABOLIQUES DANS LINDUCTION DE LOPERON LACTOSE CHEZ ESCHERICHIA COLI by BURSTEIN C (1965)
  11. CARUTHERS H. M., DECIPHERING THE PROTEIN-DNA RECOGNITION CODE, ACCOUNTS OF CHEMICAL RESEARCH 13, 155 (1980). (10.1021/ar50149a005) / ACCOUNTS OF CHEMICAL RESEARCH / DECIPHERING THE PROTEIN-DNA RECOGNITION CODE by CARUTHERS H. M. (1980)
  12. CHAKERIAN E. A., EVIDENCE FOR LEUCINE ZIPPER MOTIF IN LACTOSE REPRESSOR PROTEIN, JOURNAL OF BIOLOGICAL CHEMISTRY 266, 1371 (1991). (10.1016/S0021-9258(18)52302-4) / JOURNAL OF BIOLOGICAL CHEMISTRY / EVIDENCE FOR LEUCINE ZIPPER MOTIF IN LACTOSE REPRESSOR PROTEIN by CHAKERIAN E. A. (1991)
  13. CHAKERIAN E. A., CHARACTERIZATION OF MUTATIONS IN OLIGOMERIZATION DOMAIN OF LAC REPRESSOR PROTEIN, JOURNAL OF BIOLOGICAL CHEMISTRY 266, 22206 (1991). (10.1016/S0021-9258(18)54555-5) / JOURNAL OF BIOLOGICAL CHEMISTRY / CHARACTERIZATION OF MUTATIONS IN OLIGOMERIZATION DOMAIN OF LAC REPRESSOR PROTEIN by CHAKERIAN E. A. (1991)
  14. CHANG G, USING GENETIC ALGORITHMS FOR SOLVING HEAVY-ATOM SITES, ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 50, 667 (1994). (10.1107/S0907444994000727) / ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY / USING GENETIC ALGORITHMS FOR SOLVING HEAVY-ATOM SITES by CHANG G (1994)
  15. CHANG I. W., LYSINE-84 IS AT THE SUBUNIT INTERFACE OF LAC REPRESSOR PROTEIN, JOURNAL OF BIOLOGICAL CHEMISTRY 268, 17613 (1993). (10.1016/S0021-9258(19)85376-0) / JOURNAL OF BIOLOGICAL CHEMISTRY / LYSINE-84 IS AT THE SUBUNIT INTERFACE OF LAC REPRESSOR PROTEIN by CHANG I. W. (1993)
  16. CHEN J, SUBUNIT DISSOCIATION AFFECTS DNA-BINDING IN A DIMERIC LAC REPRESSOR PRODUCED BY C-TERMINAL DELETION, BIOCHEMISTRY 33, 8728 (1994). (10.1021/bi00195a014) / BIOCHEMISTRY / SUBUNIT DISSOCIATION AFFECTS DNA-BINDING IN A DIMERIC LAC REPRESSOR PRODUCED BY C-TERMINAL DELETION by CHEN J (1994)
  17. CHEN J, WILD-TYPE OPERATOR BINDING AND ALTERED COOPERATIVITY FOR INDUCER BINDING OF LAC REPRESSOR DIMER MUTANT R3, JOURNAL OF BIOLOGICAL CHEMISTRY 269, 12482 (1994). (10.1016/S0021-9258(18)99900-X) / JOURNAL OF BIOLOGICAL CHEMISTRY / WILD-TYPE OPERATOR BINDING AND ALTERED COOPERATIVITY FOR INDUCER BINDING OF LAC REPRESSOR DIMER MUTANT R3 by CHEN J (1994)
  18. CHUPRINA P. V., STRUCTURE OF THE COMPLEX OF LAC REPRESSOR HEADPIECE AND AN 11 BASE-PAIR HALF-OPERATOR DETERMINED BY NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY AND RESTRAINED MOLECULAR-DYNAMICS, JOURNAL OF MOLECULAR BIOLOGY 234, 446 (1993). (10.1006/jmbi.1993.1598) / JOURNAL OF MOLECULAR BIOLOGY / STRUCTURE OF THE COMPLEX OF LAC REPRESSOR HEADPIECE AND AN 11 BASE-PAIR HALF-OPERATOR DETERMINED BY NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY AND RESTRAINED MOLECULAR-DYNAMICS by CHUPRINA P. V. (1993)
  19. EISMANN R. E., LAC REPRESSOR FORMS STABLE LOOPS INVITRO WITH SUPERCOILED WILD-TYPE LAC DNA CONTAINING ALL 3 NATURAL LAC OPERATORS, JOURNAL OF MOLECULAR BIOLOGY 213, 763 (1990). (10.1016/S0022-2836(05)80262-1) / JOURNAL OF MOLECULAR BIOLOGY / LAC REPRESSOR FORMS STABLE LOOPS INVITRO WITH SUPERCOILED WILD-TYPE LAC DNA CONTAINING ALL 3 NATURAL LAC OPERATORS by EISMANN R. E. (1990)
  20. FAIRMAN R, CHARACTERIZATION OF A NEW 4-CHAIN COILED-COIL - INFLUENCE OF CHAIN-LENGTH ON STABILITY, PROTEIN SCIENCE 4, 1457 (1995). (10.1002/pro.5560040803) / PROTEIN SCIENCE / CHARACTERIZATION OF A NEW 4-CHAIN COILED-COIL - INFLUENCE OF CHAIN-LENGTH ON STABILITY by FAIRMAN R (1995)
  21. FARABAUGH J. P., SEQUENCE OF LACI GENE, NATURE 274, 765 (1978). (10.1038/274765a0) / NATURE / SEQUENCE OF LACI GENE by FARABAUGH J. P. (1978)
  22. FICKERT R, HOW LAC REPRESSOR FINDS LAC OPERATOR INVITRO, JOURNAL OF MOLECULAR BIOLOGY 226, 59 (1992). (10.1016/0022-2836(92)90124-3) / JOURNAL OF MOLECULAR BIOLOGY / HOW LAC REPRESSOR FINDS LAC OPERATOR INVITRO by FICKERT R (1992)
  23. FLASHNER Y, DUAL MECHANISM OF REPRESSION AT A DISTANCE IN THE LAC OPERON, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85, 8968 (1988). (10.1073/pnas.85.23.8968) / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA / DUAL MECHANISM OF REPRESSION AT A DISTANCE IN THE LAC OPERON by FLASHNER Y (1988)
  24. 10.1126/science.7792597
  25. FUREY W, AM CRYSTALLOGR ASS 2 18, 73 (1990). / AM CRYSTALLOGR ASS 2 by FUREY W (1990)
  26. GEISLER N, ESCHERICHIA-COLI LACTOSE REPRESSOR - ISOLATION OF 2 DIFFERENT HOMOGENEOUS HEADPIECES AND EXISTENCE OF A HINGE REGION BETWEEN RESIDUES 50 AND 60 IN REPRESSOR MOLECULE, FEBS LETTERS 87, 215 (1978). (10.1016/0014-5793(78)80335-4) / FEBS LETTERS / ESCHERICHIA-COLI LACTOSE REPRESSOR - ISOLATION OF 2 DIFFERENT HOMOGENEOUS HEADPIECES AND EXISTENCE OF A HINGE REGION BETWEEN RESIDUES 50 AND 60 IN REPRESSOR MOLECULE by GEISLER N (1978)
  27. GILBERT W, ISOLATION OF LAC REPRESSOR, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 56, 1891 (1966). (10.1073/pnas.56.6.1891) / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA / ISOLATION OF LAC REPRESSOR by GILBERT W (1966)
  28. GILBERT W, NUCLEOTIDE-SEQUENCE OF LAC OPERATOR, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 70, 3581 (1973). (10.1073/pnas.70.12.3581) / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA / NUCLEOTIDE-SEQUENCE OF LAC OPERATOR by GILBERT W (1973)
  29. GILBERT W, PROTEIN LIGAND INTER: 193 (1975). / PROTEIN LIGAND INTER by GILBERT W (1975)
  30. HARRISON C. S., A STRUCTURAL TAXONOMY OF DNA-BINDING DOMAINS, NATURE 353, 715 (1991). (10.1038/353715a0) / NATURE / A STRUCTURAL TAXONOMY OF DNA-BINDING DOMAINS by HARRISON C. S. (1991)
  31. HAUROWITZ F, HOPPESEYLERS Z PHYSL 254, 268 (1938). / HOPPESEYLERS Z PHYSL by HAUROWITZ F (1938)
  32. HUDSON M. J., COOPERATIVE INTERACTIONS BETWEEN THE CATABOLITE GENE ACTIVATOR PROTEIN AND THE LAC REPRESSOR AT THE LACTOSE PROMOTER, JOURNAL OF MOLECULAR BIOLOGY 214, 381 (1990). (10.1016/0022-2836(90)90188-R) / JOURNAL OF MOLECULAR BIOLOGY / COOPERATIVE INTERACTIONS BETWEEN THE CATABOLITE GENE ACTIVATOR PROTEIN AND THE LAC REPRESSOR AT THE LACTOSE PROMOTER by HUDSON M. J. (1990)
  33. JACOB F, ON REGULATION OF GENE ACTIVITY, COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY 26, 193 (1961). (10.1101/SQB.1961.026.01.024) / COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY / ON REGULATION OF GENE ACTIVITY by JACOB F (1961)
  34. JACOB F, GENETIC REGULATORY MECHANISMS IN SYNTHESIS OF PROTEINS, JOURNAL OF MOLECULAR BIOLOGY 3, 318 (1961). (10.1016/S0022-2836(61)80072-7) / JOURNAL OF MOLECULAR BIOLOGY / GENETIC REGULATORY MECHANISMS IN SYNTHESIS OF PROTEINS by JACOB F (1961)
  35. JAREMA MAC, GENETIC ASSIGNMENT OF RESONANCES IN THE NMR-SPECTRUM OF A PROTEIN - LAC REPRESSOR, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 78, 2707 (1981). (10.1073/pnas.78.5.2707) / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES / GENETIC ASSIGNMENT OF RESONANCES IN THE NMR-SPECTRUM OF A PROTEIN - LAC REPRESSOR by JAREMA MAC (1981)
  36. JOBE A, LAC REPRESSOR-OPERATOR INTERACTION .6. NATURAL INDUCER OF LAC OPERON, JOURNAL OF MOLECULAR BIOLOGY 69, 397 (1972). (10.1016/0022-2836(72)90253-7) / JOURNAL OF MOLECULAR BIOLOGY / LAC REPRESSOR-OPERATOR INTERACTION .6. NATURAL INDUCER OF LAC OPERON by JOBE A (1972)
  37. KABSCH W, EVALUATION OF SINGLE-CRYSTAL X-RAY-DIFFRACTION DATA FROM A POSITION-SENSITIVE DETECTOR, JOURNAL OF APPLIED CRYSTALLOGRAPHY 21, 916 (1988). (10.1107/S0021889888007903) / JOURNAL OF APPLIED CRYSTALLOGRAPHY / EVALUATION OF SINGLE-CRYSTAL X-RAY-DIFFRACTION DATA FROM A POSITION-SENSITIVE DETECTOR by KABSCH W (1988)
  38. KIM R, DIRECT MEASUREMENT OF DNA UNWINDING ANGLE IN SPECIFIC INTERACTION BETWEEN LAC OPERATOR AND REPRESSOR, COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY 47, 451 (1982). (10.1101/SQB.1983.047.01.053) / COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY / DIRECT MEASUREMENT OF DNA UNWINDING ANGLE IN SPECIFIC INTERACTION BETWEEN LAC OPERATOR AND REPRESSOR by KIM R (1982)
  39. KORNBERG D. R., THE NUCLEOSOME, SCIENTIFIC AMERICAN 244, 48 (1981). (10.1038/scientificamerican0281-52) / SCIENTIFIC AMERICAN / THE NUCLEOSOME by KORNBERG D. R. (1981)
  40. 10.1107/S0021889891004399
  41. LEWIS M unpublished data.
  42. MARKIEWICZ P, GENETIC-STUDIES OF THE LAC REPRESSOR .14. ANALYSIS OF 4000 ALTERED ESCHERICHIA-COLI LAC REPRESSORS REVEALS ESSENTIAL AND NONESSENTIAL RESIDUES, AS WELL AS SPACERS WHICH DO NOT REQUIRE A SPECIFIC SEQUENCE, JOURNAL OF MOLECULAR BIOLOGY 240, 421 (1994). (10.1006/jmbi.1994.1458) / JOURNAL OF MOLECULAR BIOLOGY / GENETIC-STUDIES OF THE LAC REPRESSOR .14. ANALYSIS OF 4000 ALTERED ESCHERICHIA-COLI LAC REPRESSORS REVEALS ESSENTIAL AND NONESSENTIAL RESIDUES, AS WELL AS SPACERS WHICH DO NOT REQUIRE A SPECIFIC SEQUENCE by MARKIEWICZ P (1994)
  43. MESSERSCHMIDT A, CRYSTAL ORIENTATION AND X-RAY PATTERN PREDICTION ROUTINES FOR AREA-DETECTOR DIFFRACTOMETER SYSTEMS IN MACROMOLECULAR CRYSTALLOGRAPHY, JOURNAL OF APPLIED CRYSTALLOGRAPHY 20, 306 (1987). (10.1107/S002188988708662X) / JOURNAL OF APPLIED CRYSTALLOGRAPHY / CRYSTAL ORIENTATION AND X-RAY PATTERN PREDICTION ROUTINES FOR AREA-DETECTOR DIFFRACTOMETER SYSTEMS IN MACROMOLECULAR CRYSTALLOGRAPHY by MESSERSCHMIDT A (1987)
  44. MILLER H. J., GENETIC-STUDIES OF THE LAC REPRESSOR .9. GENERATION OF ALTERED PROTEINS BY THE SUPPRESSION OF NONSENSE MUTATIONS, JOURNAL OF MOLECULAR BIOLOGY 131, 191 (1979). / JOURNAL OF MOLECULAR BIOLOGY / GENETIC-STUDIES OF THE LAC REPRESSOR .9. GENERATION OF ALTERED PROTEINS BY THE SUPPRESSION OF NONSENSE MUTATIONS by MILLER H. J. (1979)
  45. MILLER H. J., GENETIC-STUDIES OF THE LAC REPRESSOR .12. AMINO-ACID REPLACEMENTS IN THE DNA-BINDING DOMAIN OF THE ESCHERICHIA-COLI LAC REPRESSOR, JOURNAL OF MOLECULAR BIOLOGY 180, 205 (1984). / JOURNAL OF MOLECULAR BIOLOGY / GENETIC-STUDIES OF THE LAC REPRESSOR .12. AMINO-ACID REPLACEMENTS IN THE DNA-BINDING DOMAIN OF THE ESCHERICHIA-COLI LAC REPRESSOR by MILLER H. J. (1984)
  46. MILLER H. J., LACTOSE OPERON (1970). / LACTOSE OPERON by MILLER H. J. (1970)
  47. MILLER H. J., OPERON (1980). / OPERON by MILLER H. J. (1980)
  48. MONOD J, ALLOSTERIC PROTEINS AND CELLULAR CONTROL SYSTEMS, JOURNAL OF MOLECULAR BIOLOGY 6, 306 (1963). (10.1016/S0022-2836(63)80091-1) / JOURNAL OF MOLECULAR BIOLOGY / ALLOSTERIC PROTEINS AND CELLULAR CONTROL SYSTEMS by MONOD J (1963)
  49. MONOD J, THESIS U PARIS (1942). / THESIS U PARIS by MONOD J (1942)
  50. 10.1126/science.3090685
  51. MOWBRAY L. S., 1-BULLET-7 ANGSTROM X-RAY STRUCTURE OF THE PERIPLASMIC RIBOSE RECEPTOR FROM ESCHERICHIA-COLI, JOURNAL OF MOLECULAR BIOLOGY 225, 155 (1992). (10.1016/0022-2836(92)91033-L) / JOURNAL OF MOLECULAR BIOLOGY / 1-BULLET-7 ANGSTROM X-RAY STRUCTURE OF THE PERIPLASMIC RIBOSE RECEPTOR FROM ESCHERICHIA-COLI by MOWBRAY L. S. (1992)
  52. MULLERHILL B, SPECIFICITY OF INDUCTION OF ENZYMES OF LAC OPERON IN ESCHERICHIA COLI, JOURNAL OF MOLECULAR BIOLOGY 10, 303 (1964). (10.1016/S0022-2836(64)80049-8) / JOURNAL OF MOLECULAR BIOLOGY / SPECIFICITY OF INDUCTION OF ENZYMES OF LAC OPERON IN ESCHERICHIA COLI by MULLERHILL B (1964)
  53. NAVAZA J, AMORE - AN AUTOMATED PACKAGE FOR MOLECULAR REPLACEMENT, ACTA CRYSTALLOGRAPHICA SECTION A 50, 157 (1994). (10.1107/S0108767393007597) / ACTA CRYSTALLOGRAPHICA SECTION A / AMORE - AN AUTOMATED PACKAGE FOR MOLECULAR REPLACEMENT by NAVAZA J (1994)
  54. OEHLER S, THE 3 OPERATORS OF THE LAC OPERON COOPERATE IN REPRESSION, EMBO JOURNAL 9, 973 (1990). (10.1002/j.1460-2075.1990.tb08199.x) / EMBO JOURNAL / THE 3 OPERATORS OF THE LAC OPERON COOPERATE IN REPRESSION by OEHLER S (1990)
  55. OTWINOWSKI Z, UNPUB HKL PROGRAM SU . / UNPUB HKL PROGRAM SU by OTWINOWSKI Z
  56. PACE C. H., LAC REPRESSOR - CRYSTALLIZATION OF INTACT TETRAMER AND ITS COMPLEXES WITH INDUCER AND OPERATOR DNA, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 87, 1870 (1990). (10.1073/pnas.87.5.1870) / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA / LAC REPRESSOR - CRYSTALLIZATION OF INTACT TETRAMER AND ITS COMPLEXES WITH INDUCER AND OPERATOR DNA by PACE C. H. (1990)
  57. PERUTZ F. M., STRUCTURE OF HAEMOGLOBIN - X-RAY EXAMINATION OF REDUCED HORSE HAEMOGLOBIN, NATURE 203, 687 (1964). (10.1038/203687a0) / NATURE / STRUCTURE OF HAEMOGLOBIN - X-RAY EXAMINATION OF REDUCED HORSE HAEMOGLOBIN by PERUTZ F. M. (1964)
  58. PLATT T, LAC REPRESSOR - SPECIFIC PROTEOLYTIC DESTRUCTION OF NH2-TERMINAL REGION AND LOSS OF DEOXYRIBONUCLEIC ACID-BINDING ACTIVITY, JOURNAL OF BIOLOGICAL CHEMISTRY 248, 110 (1973). / JOURNAL OF BIOLOGICAL CHEMISTRY / LAC REPRESSOR - SPECIFIC PROTEOLYTIC DESTRUCTION OF NH2-TERMINAL REGION AND LOSS OF DEOXYRIBONUCLEIC ACID-BINDING ACTIVITY by PLATT T (1973)
  59. RIGGS D. A., LAC REPRESSOR-OPERATOR INTERACTION .3. KINETIC STUDIES, JOURNAL OF MOLECULAR BIOLOGY 53, 401 (1970). (10.1016/0022-2836(70)90074-4) / JOURNAL OF MOLECULAR BIOLOGY / LAC REPRESSOR-OPERATOR INTERACTION .3. KINETIC STUDIES by RIGGS D. A. (1970)
  60. ROYER A. C., MACROMOLECULAR BINDING EQUILIBRIA IN THE LAC REPRESSOR SYSTEM - STUDIES USING HIGH-PRESSURE FLUORESCENCE SPECTROSCOPY, BIOCHEMISTRY 29, 4959 (1990). (10.1021/bi00472a028) / BIOCHEMISTRY / MACROMOLECULAR BINDING EQUILIBRIA IN THE LAC REPRESSOR SYSTEM - STUDIES USING HIGH-PRESSURE FLUORESCENCE SPECTROSCOPY by ROYER A. C. (1990)
  61. SACK S. J., CHAIN - A CRYSTALLOGRAPHIC MODELING PROGRAM, JOURNAL OF MOLECULAR GRAPHICS 6, 224 (1988). (10.1016/S0263-7855(98)80040-4) / JOURNAL OF MOLECULAR GRAPHICS / CHAIN - A CRYSTALLOGRAPHIC MODELING PROGRAM by SACK S. J. (1988)
  62. SADLER R. J., A PERFECTLY SYMMETRIC LAC OPERATOR BINDS THE LAC REPRESSOR VERY TIGHTLY, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 80, 6785 (1983). (10.1073/pnas.80.22.6785) / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES / A PERFECTLY SYMMETRIC LAC OPERATOR BINDS THE LAC REPRESSOR VERY TIGHTLY by SADLER R. J. (1983)
  63. 10.1126/science.3353710
  64. SCHMITZ A, MUTATIONS AFFECTING QUATERNARY STRUCTURE OF LAC REPRESSOR, JOURNAL OF BIOLOGICAL CHEMISTRY 251, 3359 (1976). (10.1016/S0021-9258(17)33445-2) / JOURNAL OF BIOLOGICAL CHEMISTRY / MUTATIONS AFFECTING QUATERNARY STRUCTURE OF LAC REPRESSOR by SCHMITZ A (1976)
  65. SCHMITZ A, INTERACTION OF RNA-POLYMERASE AND LAC REPRESSOR WITH THE LAC CONTROL REGION, NUCLEIC ACIDS RESEARCH 6, 111 (1979). (10.1093/nar/6.1.111) / NUCLEIC ACIDS RESEARCH / INTERACTION OF RNA-POLYMERASE AND LAC REPRESSOR WITH THE LAC CONTROL REGION by SCHMITZ A (1979)
  66. SCHULTZ C. S., CRYSTAL-STRUCTURE OF A CAP-DNA COMPLEX - THE DNA IS BENT BY 90-DEGREES, SCIENCE 253, 1001 (1991). (10.1126/science.1653449) / SCIENCE / CRYSTAL-STRUCTURE OF A CAP-DNA COMPLEX - THE DNA IS BENT BY 90-DEGREES by SCHULTZ C. S. (1991)
  67. 10.1126/science.7973627
  68. SHIGETA R, ISOMORPHOUS BINDING OF MERCURY-SUBSTITUTED THIOSACCHARIDES TO PERTUSSIS TOXIN CRYSTALS YIELDS CRYSTALLOGRAPHIC PHASES, ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 50, 71 (1994). (10.1107/S0907444993009382) / ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY / ISOMORPHOUS BINDING OF MERCURY-SUBSTITUTED THIOSACCHARIDES TO PERTUSSIS TOXIN CRYSTALS YIELDS CRYSTALLOGRAPHIC PHASES by SHIGETA R (1994)
  69. SIEBENLIST U, ESCHERICHIA-COLI RNA-POLYMERASE INTERACTS HOMOLOGOUSLY WITH 2 DIFFERENT PROMOTERS, CELL 20, 269 (1980). (10.1016/0092-8674(80)90613-3) / CELL / ESCHERICHIA-COLI RNA-POLYMERASE INTERACTS HOMOLOGOUSLY WITH 2 DIFFERENT PROMOTERS by SIEBENLIST U (1980)
  70. SIMONS A, POSSIBLE IDEAL LAC OPERATOR - ESCHERICHIA-COLI LAC OPERATOR-LIKE SEQUENCES FROM EUKARYOTIC GENOMES LACK THE CENTRAL G-C PAIR, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 81, 1624 (1984). (10.1073/pnas.81.6.1624) / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES / POSSIBLE IDEAL LAC OPERATOR - ESCHERICHIA-COLI LAC OPERATOR-LIKE SEQUENCES FROM EUKARYOTIC GENOMES LACK THE CENTRAL G-C PAIR by SIMONS A (1984)
  71. STRANEY B. S., LAC REPRESSOR IS A TRANSIENT GENE-ACTIVATING PROTEIN, CELL 51, 699 (1987). (10.1016/0092-8674(87)90093-6) / CELL / LAC REPRESSOR IS A TRANSIENT GENE-ACTIVATING PROTEIN by STRANEY B. S. (1987)
  72. VOET D, BIOCHEMISTRY (1990). / BIOCHEMISTRY by VOET D (1990)
  73. WADEJARDETZKY N, J MOL BIOL 128, 259 (1978). (10.1016/0022-2836(79)90129-3) / J MOL BIOL by WADEJARDETZKY N (1978)
  74. WEICKERT J. M., A FAMILY OF BACTERIAL REGULATORS HOMOLOGOUS TO GAL AND LAC REPRESSORS, JOURNAL OF BIOLOGICAL CHEMISTRY 267, 15869 (1992). (10.1016/S0021-9258(19)49615-4) / JOURNAL OF BIOLOGICAL CHEMISTRY / A FAMILY OF BACTERIAL REGULATORS HOMOLOGOUS TO GAL AND LAC REPRESSORS by WEICKERT J. M. (1992)
  75. ZUBAY G, MECHANISM OF ACTIVATION OF CATABOLITE-SENSITIVE GENES - A POSITIVE CONTROL SYSTEM, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 66, 104 (1970). (10.1073/pnas.66.1.104) / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA / MECHANISM OF ACTIVATION OF CATABOLITE-SENSITIVE GENES - A POSITIVE CONTROL SYSTEM by ZUBAY G (1970)
Dates
Type When
Created 18 years, 10 months ago (Oct. 27, 2006, 2:30 p.m.)
Deposited 1 year, 7 months ago (Jan. 12, 2024, 4:33 p.m.)
Indexed 3 days, 1 hour ago (Aug. 27, 2025, 12:03 p.m.)
Issued 29 years, 5 months ago (March 1, 1996)
Published 29 years, 5 months ago (March 1, 1996)
Published Print 29 years, 5 months ago (March 1, 1996)
Funders 0

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@article{Lewis_1996, title={Crystal Structure of the Lactose Operon Repressor and Its Complexes with DNA and Inducer}, volume={271}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.271.5253.1247}, DOI={10.1126/science.271.5253.1247}, number={5253}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Lewis, Mitchell and Chang, Geoffrey and Horton, Nancy C. and Kercher, Michele A. and Pace, Helen C. and Schumacher, Maria A. and Brennan, Richard G. and Lu, Ponzy}, year={1996}, month=mar, pages={1247–1254} }