DOI: 10.1126/science.271.5249.653. Use-Dependent Blockers and Exit Rate of the Last Ion from the Multi-Ion Pore of a K <sup>+</sup> Channel

Use-Dependent Blockers and Exit Rate of the Last Ion from the Multi-Ion Pore of a K + Channel

10.1126/science.271.5249.653

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Quaternary ammonium blockers inhibit many voltage-activated potassium (K + ) channels from the intracellular side. When applied to Drosophila Shaker potassium channels expressed in mammalian cells, these rapidly reversible blockers produced use-dependent inhibition through an unusual mechanism-they promoted an intrinsic conformational change known as C-type inactivation, from which recovery is slow. The blockers did so by cutting off potassium ion flow to a site in the pore, which then emptied at a rate of 10 5 ions per second. This slow rate probably reflected the departure of the last ion from the multi-ion pore: Permeation of ions (at 10 7 per second) occurs rapidly because of ion-ion repulsion, but the last ion to leave would experience no such repulsion.

Bibliography

Baukrowitz, T., & Yellen, G. (1996). Use-Dependent Blockers and Exit Rate of the Last Ion from the Multi-Ion Pore of a K + Channel. Science, 271(5249), 653â656.

Authors 2

Thomas Baukrowitz (first)
Gary Yellen (additional)

References 28 Referenced 194

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 2:30 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 6:15 p.m.)
Indexed	3 weeks, 6 days ago (Aug. 2, 2025, 12:18 a.m.)
Issued	29 years, 6 months ago (Feb. 2, 1996)
Published	29 years, 6 months ago (Feb. 2, 1996)
Published Print	29 years, 6 months ago (Feb. 2, 1996)

Funders 0

None

BibTeX

@article{Baukrowitz_1996, title={Use-Dependent Blockers and Exit Rate of the Last Ion from the Multi-Ion Pore of a K + Channel}, volume={271}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.271.5249.653}, DOI={10.1126/science.271.5249.653}, number={5249}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Baukrowitz, Thomas and Yellen, Gary}, year={1996}, month=feb, pages={653–656} }

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