Catalysis of Amide Proton Exchange by the Molecular Chaperones GroEL and SecB
10.1126/science.271.5249.642
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Hydrogen-deuterium exchange of 39 amide protons of Bacillus amyloliquefaciens ribonuclease (barnase) was analyzed by two-dimensional nuclear magnetic resonance in the presence of micromolar concentrations of the molecular chaperones GroEL and SecB. Both chaperones bound to native barnase under physiological conditions and catalyzed exchange of deeply buried amide protons with solvent. Such exchange required complete unfolding of barnase, which occurred in the complex with the chaperones. Subsequent collapse of unfolded barnase to the exchange-protected folding intermediate was markedly slowed in the presence of GroEL or SecB. Thus, both chaperones have the potential to correct misfolding in proteins by annealing.

Bibliography

Zahn, R., Perrett, S., Stenberg, G., & Fersht, A. R. (1996). Catalysis of Amide Proton Exchange by the Molecular Chaperones GroEL and SecB. Science, 271(5249), 642–645.

Authors 4
  1. Ralph Zahn (first)
  2. Sarah Perrett (additional)
  3. Gun Stenberg (additional)
  4. Alan R. Fersht (additional)
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Dates
Type When
Created 18 years, 10 months ago (Oct. 27, 2006, 2:30 p.m.)
Deposited 1 year, 7 months ago (Jan. 12, 2024, 6:16 p.m.)
Indexed 1 month, 3 weeks ago (July 2, 2025, 2:46 p.m.)
Issued 29 years, 6 months ago (Feb. 2, 1996)
Published 29 years, 6 months ago (Feb. 2, 1996)
Published Print 29 years, 6 months ago (Feb. 2, 1996)
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@article{Zahn_1996, title={Catalysis of Amide Proton Exchange by the Molecular Chaperones GroEL and SecB}, volume={271}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.271.5249.642}, DOI={10.1126/science.271.5249.642}, number={5249}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Zahn, Ralph and Perrett, Sarah and Stenberg, Gun and Fersht, Alan R.}, year={1996}, month=feb, pages={642–645} }