DOI: 10.1126/science.270.5244.1967. High-Resolution Microcoil <sup>1</sup> H-NMR for Mass-Limited, Nanoliter-Volume Samples

High-Resolution Microcoil 1 H-NMR for Mass-Limited, Nanoliter-Volume Samples

10.1126/science.270.5244.1967

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

High-resolution, proton nuclear magnetic resonance (NMR) spectra of 5-nanoliter samples have been obtained with much higher mass sensitivity [signal-to-noise ratio (S/N) per micromole] than with traditional methods. Arginine and sucrose show a mean sensitivity enhancement of 130 compared to 278-microliter samples run in a 5-millimeter tube in a conventional, commercial probe. This can reduce data acquisition time by a factor of >16,000 or reduce the needed sample mass by a factor of about 130. A linewidth of 0.6 hertz was achieved on a 300-megahertz spectrometer by matching the magnetic susceptibility of the medium that surrounds the detection cell to that of the copper coil. For sucrose, the limit of detection (defined at S/N = 3) was 19 nanograms (56 picomoles) for a 1-minute data acquisition. This technique should prove useful with mass-limited samples and for use as a detector in capillary separations.

Bibliography

Olson, D. L., Peck, T. L., Webb, A. G., Magin, R. L., & Sweedler, J. V. (1995). High-Resolution Microcoil 1 H-NMR for Mass-Limited, Nanoliter-Volume Samples. Science, 270(5244), 1967â1970.

Authors 5

Dean L. Olson (first)
Timothy L. Peck (additional)
Andrew G. Webb (additional)
Richard L. Magin (additional)
Jonathan V. Sweedler (additional)

References 26 Referenced 448

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BURLINGAME A.L., MASS-SPECTROMETRY, ANALYTICAL CHEMISTRY 66, R634 (1994). (10.1021/ac00084a024) / ANALYTICAL CHEMISTRY / MASS-SPECTROMETRY by BURLINGAME A.L. (1994)
CONOVER W.W., TOPICS CARBON 13 NMR 4, 37 (1984). / TOPICS CARBON 13 NMR by CONOVER W.W. (1984)
DASGUPTA P.K., ELECTROOSMOSIS - A RELIABLE FLUID PROPULSION SYSTEM FOR FLOW-INJECTION ANALYSIS, ANALYTICAL CHEMISTRY 66, 1792 (1994). (10.1021/ac00083a004) / ANALYTICAL CHEMISTRY / ELECTROOSMOSIS - A RELIABLE FLUID PROPULSION SYSTEM FOR FLOW-INJECTION ANALYSIS by DASGUPTA P.K. (1994)
DEROME A.E., MODERN NMR TECHNIQUE (1987). / MODERN NMR TECHNIQUE by DEROME A.E. (1987)
FUKS L.F., SUSCEPTIBILITY, LINESHAPE, AND SHIMMING IN HIGH-RESOLUTION NMR, JOURNAL OF MAGNETIC RESONANCE 100, 229 (1992). / JOURNAL OF MAGNETIC RESONANCE / SUSCEPTIBILITY, LINESHAPE, AND SHIMMING IN HIGH-RESOLUTION NMR by FUKS L.F. (1992)
HINTON D.P., DIFFUSION ORDERED 2D-NMR SPECTROSCOPY OF PHOSPHOLIPID-VESICLES - DETERMINATION OF VESICLE SIZE DISTRIBUTIONS, JOURNAL OF PHYSICAL CHEMISTRY 97, 9064 (1993). (10.1021/j100137a038) / JOURNAL OF PHYSICAL CHEMISTRY / DIFFUSION ORDERED 2D-NMR SPECTROSCOPY OF PHOSPHOLIPID-VESICLES - DETERMINATION OF VESICLE SIZE DISTRIBUTIONS by HINTON D.P. (1993)
HOLZ M, MODIFICATION OF THE PULSED MAGNETIC-FIELD GRADIENT-METHOD FOR THE DETERMINATION OF LOW VELOCITIES BY NMR, JOURNAL OF MAGNETIC RESONANCE 69, 108 (1986). / JOURNAL OF MAGNETIC RESONANCE / MODIFICATION OF THE PULSED MAGNETIC-FIELD GRADIENT-METHOD FOR THE DETERMINATION OF LOW VELOCITIES BY NMR by HOLZ M (1986)
HOULT D.I., SIGNAL-TO-NOISE RATIO OF NUCLEAR MAGNETIC-RESONANCE EXPERIMENT, JOURNAL OF MAGNETIC RESONANCE 24, 71 (1976). / JOURNAL OF MAGNETIC RESONANCE / SIGNAL-TO-NOISE RATIO OF NUCLEAR MAGNETIC-RESONANCE EXPERIMENT by HOULT D.I. (1976)
HOULT D.I., SENSITIVITY OF THE ZEUGMATOGRAPHIC EXPERIMENT INVOLVING HUMAN SAMPLES, JOURNAL OF MAGNETIC RESONANCE 34, 425 (1979). / JOURNAL OF MAGNETIC RESONANCE / SENSITIVITY OF THE ZEUGMATOGRAPHIC EXPERIMENT INVOLVING HUMAN SAMPLES by HOULT D.I. (1979)
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Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 2:19 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 4:45 p.m.)
Indexed	4 weeks, 2 days ago (July 30, 2025, 10:43 a.m.)
Issued	29 years, 8 months ago (Dec. 22, 1995)
Published	29 years, 8 months ago (Dec. 22, 1995)
Published Print	29 years, 8 months ago (Dec. 22, 1995)

Funders 0

None

BibTeX

@article{Olson_1995, title={High-Resolution Microcoil 1 H-NMR for Mass-Limited, Nanoliter-Volume Samples}, volume={270}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.270.5244.1967}, DOI={10.1126/science.270.5244.1967}, number={5244}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Olson, Dean L. and Peck, Timothy L. and Webb, Andrew G. and Magin, Richard L. and Sweedler, Jonathan V.}, year={1995}, month=dec, pages={1967–1970} }

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