DOI: 10.1126/science.270.5243.1828. Control of Cell Fate by a Deubiquitinating Enzyme Encoded by the <i>fat facets</i> Gene

Control of Cell Fate by a Deubiquitinating Enzyme Encoded by the fat facets Gene

10.1126/science.270.5243.1828

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Ubiquitin is a highly conserved polypeptide found in all eukaryotes. The major function of ubiquitin is to target proteins for complete or partial degradation by a multisubunit protein complex called the proteasome. Here, the Drosophila fat facets gene, which is required for the appropriate determination of particular cells in the fly eye, was shown to encode a ubiquitin-specific protease (Ubp), an enzyme that cleaves ubiquitin from ubiquitin-protein conjugates. The Fat facets protein (FAF) acts as a regulatory Ubp that prevents degradation of its substrate by the proteasome. Flies bearing fat facets gene mutations were used to show that a Ubp is cell type- and substrate-specific and a regulator of cell fate decisions in a multicellular organism.

Bibliography

Huang, Y., Baker, R. T., & Fischer-Vize, J. A. (1995). Control of Cell Fate by a Deubiquitinating Enzyme Encoded by the fat facets Gene. Science, 270(5243), 1828â1831.

Authors 3

Yongzhao Huang (first)
Rohan T. Baker (additional)
Janice A. Fischer-Vize (additional)

References 31 Referenced 219

BAKER R.T., UBIQUITIN-SPECIFIC PROTEASES OF SACCHAROMYCES-CEREVISIAE - CLONING OF UBP2 AND UBP3, AND FUNCTIONAL-ANALYSIS OF THE UBP GENE FAMILY, JOURNAL OF BIOLOGICAL CHEMISTRY 267, 23364 (1992). (10.1016/S0021-9258(18)50100-9) / JOURNAL OF BIOLOGICAL CHEMISTRY / UBIQUITIN-SPECIFIC PROTEASES OF SACCHAROMYCES-CEREVISIAE - CLONING OF UBP2 AND UBP3, AND FUNCTIONAL-ANALYSIS OF THE UBP GENE FAMILY by BAKER R.T. (1992)
BAKER R.T. unpublished data.
BAKER R.T. unpublished data.
CIECHANOVER A, THE UBIQUITIN-PROTEASOME PROTEOLYTIC PATHWAY, CELL 79, 13 (1994). (10.1016/0092-8674(94)90396-4) / CELL / THE UBIQUITIN-PROTEASOME PROTEOLYTIC PATHWAY by CIECHANOVER A (1994)
DESHAIES R.J., UBIQUITINATION OF THE G(1) CYCLIN CLN2P BY A CDC34P-DEPENDENT PATHWAY, EMBO JOURNAL 14, 303 (1995). (10.1002/j.1460-2075.1995.tb07004.x) / EMBO JOURNAL / UBIQUITINATION OF THE G(1) CYCLIN CLN2P BY A CDC34P-DEPENDENT PATHWAY by DESHAIES R.J. (1995)
FISCHERVIZE J.A., THE FAT-FACETS GENE IS REQUIRED FOR DROSOPHILA EYE AND EMBRYO DEVELOPMENT, DEVELOPMENT 116, 985 (1992). (10.1242/dev.116.4.985) / DEVELOPMENT / THE FAT-FACETS GENE IS REQUIRED FOR DROSOPHILA EYE AND EMBRYO DEVELOPMENT by FISCHERVIZE J.A. (1992)
FISCHERVIZE J.A. unpublished data.
10.1038/349132a0
HOCHSTRASSER M, UBIQUITIN, PROTEASOMES, AND THE REGULATION OF INTRACELLULAR PROTEIN-DEGRADATION, CURRENT OPINION IN CELL BIOLOGY 7, 215 (1995). (10.1016/0955-0674(95)80031-X) / CURRENT OPINION IN CELL BIOLOGY / UBIQUITIN, PROTEASOMES, AND THE REGULATION OF INTRACELLULAR PROTEIN-DEGRADATION by HOCHSTRASSER M (1995)
KORNITZER D, REGULATED DEGRADATION OF THE TRANSCRIPTION FACTOR GCN4, EMBO JOURNAL 13, 6021 (1994). (10.1002/j.1460-2075.1994.tb06948.x) / EMBO JOURNAL / REGULATED DEGRADATION OF THE TRANSCRIPTION FACTOR GCN4 by KORNITZER D (1994)
LIS J.T., NEW HEAT-SHOCK PUFFS AND BETA-GALACTOSIDASE ACTIVITY RESULTING FROM TRANSFORMATION OF DROSOPHILA WITH AN HSP70-LACZ HYBRID GENE, CELL 35, 403 (1983). (10.1016/0092-8674(83)90173-3) / CELL / NEW HEAT-SHOCK PUFFS AND BETA-GALACTOSIDASE ACTIVITY RESULTING FROM TRANSFORMATION OF DROSOPHILA WITH AN HSP70-LACZ HYBRID GENE by LIS J.T. (1983)
MILLER H.I., CLONING AND EXPRESSION OF A YEAST UBIQUITIN-PROTEIN CLEAVING ACTIVITY IN ESCHERICHIA-COLI, BIO-TECHNOLOGY 7, 698 (1989). / BIO-TECHNOLOGY / CLONING AND EXPRESSION OF A YEAST UBIQUITIN-PROTEIN CLEAVING ACTIVITY IN ESCHERICHIA-COLI by MILLER H.I. (1989)
MURALIDHAR M.G., THE DROSOPHILA BENDLESS GENE ENCODES A NEURAL PROTEIN RELATED TO UBIQUITIN-CONJUGATING ENZYMES, NEURON 11, 253 (1993). (10.1016/0896-6273(93)90182-Q) / NEURON / THE DROSOPHILA BENDLESS GENE ENCODES A NEURAL PROTEIN RELATED TO UBIQUITIN-CONJUGATING ENZYMES by MURALIDHAR M.G. (1993)
OH C.E., BENDLESS, A DROSOPHILA GENE AFFECTING NEURONAL CONNECTIVITY, ENCODES A UBIQUITIN-CONJUGATING ENZYME HOMOLOG, JOURNAL OF NEUROSCIENCE 14, 3166 (1994). (10.1523/JNEUROSCI.14-05-03166.1994) / JOURNAL OF NEUROSCIENCE / BENDLESS, A DROSOPHILA GENE AFFECTING NEURONAL CONNECTIVITY, ENCODES A UBIQUITIN-CONJUGATING ENZYME HOMOLOG by OH C.E. (1994)
PALOMBELLA V.J., THE UBIQUITIN-PROTEASOME PATHWAY IS REQUIRED FOR PROCESSING THE NF-KAPPA-B1 PRECURSOR PROTEIN AND THE ACTIVATION OF NF-KAPPA-B, CELL 78, 773 (1994). (10.1016/S0092-8674(94)90482-0) / CELL / THE UBIQUITIN-PROTEASOME PATHWAY IS REQUIRED FOR PROCESSING THE NF-KAPPA-B1 PRECURSOR PROTEIN AND THE ACTIVATION OF NF-KAPPA-B by PALOMBELLA V.J. (1994)
PAPA F.R., THE YEAST DOA4 GENE ENCODES A DEUBIQUITINATING ENZYME RELATED TO A PRODUCT OF THE HUMAN TRE-2 ONCOGENE, NATURE 366, 313 (1993). (10.1038/366313a0) / NATURE / THE YEAST DOA4 GENE ENCODES A DEUBIQUITINATING ENZYME RELATED TO A PRODUCT OF THE HUMAN TRE-2 ONCOGENE by PAPA F.R. (1993)
PELHAM HRB, A REGULATORY UPSTREAM PROMOTER ELEMENT IN THE DROSOPHILA HSP 70 HEAT-SHOCK GENE, CELL 30, 517 (1982). (10.1016/0092-8674(82)90249-5) / CELL / A REGULATORY UPSTREAM PROMOTER ELEMENT IN THE DROSOPHILA HSP 70 HEAT-SHOCK GENE by PELHAM HRB (1982)
ROCK K.L., INHIBITORS OF THE PROTEASOME BLOCK THE DEGRADATION OF MOST CELL-PROTEINS AND THE GENERATION OF PEPTIDES PRESENTED ON MHC CLASS-I MOLECULES, CELL 78, 761 (1994). (10.1016/S0092-8674(94)90462-6) / CELL / INHIBITORS OF THE PROTEASOME BLOCK THE DEGRADATION OF MOST CELL-PROTEINS AND THE GENERATION OF PEPTIDES PRESENTED ON MHC CLASS-I MOLECULES by ROCK K.L. (1994)
SAMBROOK G, MOL CLONING LABORATO (1989). / MOL CLONING LABORATO by SAMBROOK G (1989)
SAVILLE K.J., IDENTIFICATION OF AN ESSENTIAL GENE, L(3)73AI, WITH A DOMINANT TEMPERATURE-SENSITIVE LETHAL ALLELE, ENCODING A DROSOPHILA PROTEASOME SUBUNIT, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 90, 8842 (1993). (10.1073/pnas.90.19.8842) / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA / IDENTIFICATION OF AN ESSENTIAL GENE, L(3)73AI, WITH A DOMINANT TEMPERATURE-SENSITIVE LETHAL ALLELE, ENCODING A DROSOPHILA PROTEASOME SUBUNIT by SAVILLE K.J. (1993)
SPRADLING A.C., DROSOPHILA PRACTICAL 175, (1986). / DROSOPHILA PRACTICAL by SPRADLING A.C. (1986)
THUMMEL C.S., DROS INF SERV 71, 150 (1992). / DROS INF SERV by THUMMEL C.S. (1992)
THUMMEL C.S., GENE 74, 543 (1988). (10.1016/0378-1119(88)90177-1) / GENE by THUMMEL C.S. (1988)
TOBIAS J.W., CLONING AND FUNCTIONAL-ANALYSIS OF THE UBIQUITIN-SPECIFIC PROTEASE GENE UBP1 OF SACCHAROMYCES-CEREVISIAE, JOURNAL OF BIOLOGICAL CHEMISTRY 266, 12021 (1991). (10.1016/S0021-9258(18)99059-9) / JOURNAL OF BIOLOGICAL CHEMISTRY / CLONING AND FUNCTIONAL-ANALYSIS OF THE UBIQUITIN-SPECIFIC PROTEASE GENE UBP1 OF SACCHAROMYCES-CEREVISIAE by TOBIAS J.W. (1991)
10.1126/science.1962196
TOMLINSON A, CELL FATE IN THE DROSOPHILA OMMATIDIUM, DEVELOPMENTAL BIOLOGY 123, 264 (1987). (10.1016/0012-1606(87)90448-9) / DEVELOPMENTAL BIOLOGY / CELL FATE IN THE DROSOPHILA OMMATIDIUM by TOMLINSON A (1987)
TREIER M, UBIQUITIN-DEPENDENT C-JUN DEGRADATION IN-VIVO IS MEDIATED BY THE DELTA-DOMAIN, CELL 78, 787 (1994). (10.1016/S0092-8674(94)90502-9) / CELL / UBIQUITIN-DEPENDENT C-JUN DEGRADATION IN-VIVO IS MEDIATED BY THE DELTA-DOMAIN by TREIER M (1994)
WILKINSON K.D., THE NEURON-SPECIFIC PROTEIN PGP-9.5 IS A UBIQUITIN CARBOXYL-TERMINAL HYDROLASE, SCIENCE 246, 670 (1989). (10.1126/science.2530630) / SCIENCE / THE NEURON-SPECIFIC PROTEIN PGP-9.5 IS A UBIQUITIN CARBOXYL-TERMINAL HYDROLASE by WILKINSON K.D. (1989)
WOLFF T, DEV DROSOPHILA MELAN 2, CH22 (1993). / DEV DROSOPHILA MELAN by WOLFF T (1993)
YAGLOM J, P34(CDC28)-MEDIATED CONTROL OF CLN3 CYCLIN DEGRADATION, MOLECULAR AND CELLULAR BIOLOGY 15, 731 (1995). (10.1128/MCB.15.2.731) / MOLECULAR AND CELLULAR BIOLOGY / P34(CDC28)-MEDIATED CONTROL OF CLN3 CYCLIN DEGRADATION by YAGLOM J (1995)
ZHANG N, CLONING AND ANALYSIS OF EXPRESSION OF A UBIQUITIN CARBOXYL-TERMINAL HYDROLASE EXPRESSED DURING OOGENESIS IN DROSOPHILA-MELANOGASTER, DEVELOPMENTAL BIOLOGY 157, 214 (1993). (10.1006/dbio.1993.1125) / DEVELOPMENTAL BIOLOGY / CLONING AND ANALYSIS OF EXPRESSION OF A UBIQUITIN CARBOXYL-TERMINAL HYDROLASE EXPRESSED DURING OOGENESIS IN DROSOPHILA-MELANOGASTER by ZHANG N (1993)

Dates

Type	When
Created	18 years, 9 months ago (Oct. 27, 2006, 2:19 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 4:33 p.m.)
Indexed	1 month, 2 weeks ago (July 7, 2025, 2:28 a.m.)
Issued	29 years, 8 months ago (Dec. 15, 1995)
Published	29 years, 8 months ago (Dec. 15, 1995)
Published Print	29 years, 8 months ago (Dec. 15, 1995)

Funders 0

None

BibTeX

@article{Huang_1995, title={Control of Cell Fate by a Deubiquitinating Enzyme Encoded by the fat facets Gene}, volume={270}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.270.5243.1828}, DOI={10.1126/science.270.5243.1828}, number={5243}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Huang, Yongzhao and Baker, Rohan T. and Fischer-Vize, Janice A.}, year={1995}, month=dec, pages={1828–1831} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        7
      ]
    ],
    "date-time": "2025-07-07T06:28:29Z",
    "timestamp": 1751869709599
  },
  "reference-count": 31,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "5243",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1995,
        12,
        15
      ]
    ]
  },
  "abstract": "<jats:p>\n            Ubiquitin is a highly conserved polypeptide found in all eukaryotes. The major function of ubiquitin is to target proteins for complete or partial degradation by a multisubunit protein complex called the proteasome. Here, the\n            <jats:italic>Drosophila fat facets</jats:italic>\n            gene, which is required for the appropriate determination of particular cells in the fly eye, was shown to encode a ubiquitin-specific protease (Ubp), an enzyme that cleaves ubiquitin from ubiquitin-protein conjugates. The Fat facets protein (FAF) acts as a regulatory Ubp that prevents degradation of its substrate by the proteasome. Flies bearing\n            <jats:italic>fat facets</jats:italic>\n            gene mutations were used to show that a Ubp is cell type- and substrate-specific and a regulator of cell fate decisions in a multicellular organism.\n          </jats:p>",
  "DOI": "10.1126/science.270.5243.1828",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        10,
        27
      ]
    ],
    "date-time": "2006-10-27T18:19:42Z",
    "timestamp": 1161973182000
  },
  "page": "1828-1831",
  "source": "Crossref",
  "is-referenced-by-count": 219,
  "title": "Control of Cell Fate by a Deubiquitinating Enzyme Encoded by the\n            <i>fat facets</i>\n            Gene",
  "prefix": "10.1126",
  "volume": "270",
  "author": [
    {
      "given": "Yongzhao",
      "family": "Huang",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Y. Huang and J. A. Fischer-Vize, Department of Zoology, University of Texas, Austin, TX 78712, USA."
        }
      ]
    },
    {
      "given": "Rohan T.",
      "family": "Baker",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "R. T. Baker, Molecular Genetics Group, John Curtin School of Medical Research, Australian National University, P.O. Box 334, Canberra, ACT 2601, Australia."
        }
      ]
    },
    {
      "given": "Janice A.",
      "family": "Fischer-Vize",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Y. Huang and J. A. Fischer-Vize, Department of Zoology, University of Texas, Austin, TX 78712, USA."
        }
      ]
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_2_1_2_2",
      "doi-asserted-by": "crossref",
      "first-page": "23364",
      "DOI": "10.1016/S0021-9258(18)50100-9",
      "article-title": "UBIQUITIN-SPECIFIC PROTEASES OF SACCHAROMYCES-CEREVISIAE - CLONING OF UBP2 AND UBP3, AND FUNCTIONAL-ANALYSIS OF THE UBP GENE FAMILY",
      "volume": "267",
      "author": "BAKER R.T.",
      "year": "1992",
      "unstructured": "BAKER R.T., UBIQUITIN-SPECIFIC PROTEASES OF SACCHAROMYCES-CEREVISIAE - CLONING OF UBP2 AND UBP3, AND FUNCTIONAL-ANALYSIS OF THE UBP GENE FAMILY, JOURNAL OF BIOLOGICAL CHEMISTRY 267, 23364 (1992).",
      "journal-title": "JOURNAL OF BIOLOGICAL CHEMISTRY"
    },
    {
      "key": "e_1_2_1_3_2",
      "unstructured": "BAKER R.T. unpublished data."
    },
    {
      "key": "e_1_2_1_4_2",
      "unstructured": "BAKER R.T. unpublished data."
    },
    {
      "key": "e_1_2_1_5_2",
      "doi-asserted-by": "crossref",
      "first-page": "13",
      "DOI": "10.1016/0092-8674(94)90396-4",
      "article-title": "THE UBIQUITIN-PROTEASOME PROTEOLYTIC PATHWAY",
      "volume": "79",
      "author": "CIECHANOVER A",
      "year": "1994",
      "unstructured": "CIECHANOVER A, THE UBIQUITIN-PROTEASOME PROTEOLYTIC PATHWAY, CELL 79, 13 (1994).",
      "journal-title": "CELL"
    },
    {
      "key": "e_1_2_1_6_2",
      "doi-asserted-by": "crossref",
      "first-page": "303",
      "DOI": "10.1002/j.1460-2075.1995.tb07004.x",
      "article-title": "UBIQUITINATION OF THE G(1) CYCLIN CLN2P BY A CDC34P-DEPENDENT PATHWAY",
      "volume": "14",
      "author": "DESHAIES R.J.",
      "year": "1995",
      "unstructured": "DESHAIES R.J., UBIQUITINATION OF THE G(1) CYCLIN CLN2P BY A CDC34P-DEPENDENT PATHWAY, EMBO JOURNAL 14, 303 (1995).",
      "journal-title": "EMBO JOURNAL"
    },
    {
      "key": "e_1_2_1_7_2",
      "doi-asserted-by": "crossref",
      "first-page": "985",
      "DOI": "10.1242/dev.116.4.985",
      "article-title": "THE FAT-FACETS GENE IS REQUIRED FOR DROSOPHILA EYE AND EMBRYO DEVELOPMENT",
      "volume": "116",
      "author": "FISCHERVIZE J.A.",
      "year": "1992",
      "unstructured": "FISCHERVIZE J.A., THE FAT-FACETS GENE IS REQUIRED FOR DROSOPHILA EYE AND EMBRYO DEVELOPMENT, DEVELOPMENT 116, 985 (1992).",
      "journal-title": "DEVELOPMENT"
    },
    {
      "key": "e_1_2_1_8_2",
      "unstructured": "FISCHERVIZE J.A. unpublished data."
    },
    {
      "key": "e_1_2_1_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/349132a0"
    },
    {
      "key": "e_1_2_1_10_2",
      "doi-asserted-by": "crossref",
      "first-page": "215",
      "DOI": "10.1016/0955-0674(95)80031-X",
      "article-title": "UBIQUITIN, PROTEASOMES, AND THE REGULATION OF INTRACELLULAR PROTEIN-DEGRADATION",
      "volume": "7",
      "author": "HOCHSTRASSER M",
      "year": "1995",
      "unstructured": "HOCHSTRASSER M, UBIQUITIN, PROTEASOMES, AND THE REGULATION OF INTRACELLULAR PROTEIN-DEGRADATION, CURRENT OPINION IN CELL BIOLOGY 7, 215 (1995).",
      "journal-title": "CURRENT OPINION IN CELL BIOLOGY"
    },
    {
      "key": "e_1_2_1_11_2",
      "doi-asserted-by": "crossref",
      "first-page": "6021",
      "DOI": "10.1002/j.1460-2075.1994.tb06948.x",
      "article-title": "REGULATED DEGRADATION OF THE TRANSCRIPTION FACTOR GCN4",
      "volume": "13",
      "author": "KORNITZER D",
      "year": "1994",
      "unstructured": "KORNITZER D, REGULATED DEGRADATION OF THE TRANSCRIPTION FACTOR GCN4, EMBO JOURNAL 13, 6021 (1994).",
      "journal-title": "EMBO JOURNAL"
    },
    {
      "key": "e_1_2_1_12_2",
      "doi-asserted-by": "crossref",
      "first-page": "403",
      "DOI": "10.1016/0092-8674(83)90173-3",
      "article-title": "NEW HEAT-SHOCK PUFFS AND BETA-GALACTOSIDASE ACTIVITY RESULTING FROM TRANSFORMATION OF DROSOPHILA WITH AN HSP70-LACZ HYBRID GENE",
      "volume": "35",
      "author": "LIS J.T.",
      "year": "1983",
      "unstructured": "LIS J.T., NEW HEAT-SHOCK PUFFS AND BETA-GALACTOSIDASE ACTIVITY RESULTING FROM TRANSFORMATION OF DROSOPHILA WITH AN HSP70-LACZ HYBRID GENE, CELL 35, 403 (1983).",
      "journal-title": "CELL"
    },
    {
      "key": "e_1_2_1_13_2",
      "first-page": "698",
      "article-title": "CLONING AND EXPRESSION OF A YEAST UBIQUITIN-PROTEIN CLEAVING ACTIVITY IN ESCHERICHIA-COLI",
      "volume": "7",
      "author": "MILLER H.I.",
      "year": "1989",
      "unstructured": "MILLER H.I., CLONING AND EXPRESSION OF A YEAST UBIQUITIN-PROTEIN CLEAVING ACTIVITY IN ESCHERICHIA-COLI, BIO-TECHNOLOGY 7, 698 (1989).",
      "journal-title": "BIO-TECHNOLOGY"
    },
    {
      "key": "e_1_2_1_14_2",
      "doi-asserted-by": "crossref",
      "first-page": "253",
      "DOI": "10.1016/0896-6273(93)90182-Q",
      "article-title": "THE DROSOPHILA BENDLESS GENE ENCODES A NEURAL PROTEIN RELATED TO UBIQUITIN-CONJUGATING ENZYMES",
      "volume": "11",
      "author": "MURALIDHAR M.G.",
      "year": "1993",
      "unstructured": "MURALIDHAR M.G., THE DROSOPHILA BENDLESS GENE ENCODES A NEURAL PROTEIN RELATED TO UBIQUITIN-CONJUGATING ENZYMES, NEURON 11, 253 (1993).",
      "journal-title": "NEURON"
    },
    {
      "key": "e_1_2_1_15_2",
      "doi-asserted-by": "crossref",
      "first-page": "3166",
      "DOI": "10.1523/JNEUROSCI.14-05-03166.1994",
      "article-title": "BENDLESS, A DROSOPHILA GENE AFFECTING NEURONAL CONNECTIVITY, ENCODES A UBIQUITIN-CONJUGATING ENZYME HOMOLOG",
      "volume": "14",
      "author": "OH C.E.",
      "year": "1994",
      "unstructured": "OH C.E., BENDLESS, A DROSOPHILA GENE AFFECTING NEURONAL CONNECTIVITY, ENCODES A UBIQUITIN-CONJUGATING ENZYME HOMOLOG, JOURNAL OF NEUROSCIENCE 14, 3166 (1994).",
      "journal-title": "JOURNAL OF NEUROSCIENCE"
    },
    {
      "key": "e_1_2_1_16_2",
      "doi-asserted-by": "crossref",
      "first-page": "773",
      "DOI": "10.1016/S0092-8674(94)90482-0",
      "article-title": "THE UBIQUITIN-PROTEASOME PATHWAY IS REQUIRED FOR PROCESSING THE NF-KAPPA-B1 PRECURSOR PROTEIN AND THE ACTIVATION OF NF-KAPPA-B",
      "volume": "78",
      "author": "PALOMBELLA V.J.",
      "year": "1994",
      "unstructured": "PALOMBELLA V.J., THE UBIQUITIN-PROTEASOME PATHWAY IS REQUIRED FOR PROCESSING THE NF-KAPPA-B1 PRECURSOR PROTEIN AND THE ACTIVATION OF NF-KAPPA-B, CELL 78, 773 (1994).",
      "journal-title": "CELL"
    },
    {
      "key": "e_1_2_1_17_2",
      "doi-asserted-by": "crossref",
      "first-page": "313",
      "DOI": "10.1038/366313a0",
      "article-title": "THE YEAST DOA4 GENE ENCODES A DEUBIQUITINATING ENZYME RELATED TO A PRODUCT OF THE HUMAN TRE-2 ONCOGENE",
      "volume": "366",
      "author": "PAPA F.R.",
      "year": "1993",
      "unstructured": "PAPA F.R., THE YEAST DOA4 GENE ENCODES A DEUBIQUITINATING ENZYME RELATED TO A PRODUCT OF THE HUMAN TRE-2 ONCOGENE, NATURE 366, 313 (1993).",
      "journal-title": "NATURE"
    },
    {
      "key": "e_1_2_1_18_2",
      "doi-asserted-by": "crossref",
      "first-page": "517",
      "DOI": "10.1016/0092-8674(82)90249-5",
      "article-title": "A REGULATORY UPSTREAM PROMOTER ELEMENT IN THE DROSOPHILA HSP 70 HEAT-SHOCK GENE",
      "volume": "30",
      "author": "PELHAM HRB",
      "year": "1982",
      "unstructured": "PELHAM HRB, A REGULATORY UPSTREAM PROMOTER ELEMENT IN THE DROSOPHILA HSP 70 HEAT-SHOCK GENE, CELL 30, 517 (1982).",
      "journal-title": "CELL"
    },
    {
      "key": "e_1_2_1_19_2",
      "doi-asserted-by": "crossref",
      "first-page": "761",
      "DOI": "10.1016/S0092-8674(94)90462-6",
      "article-title": "INHIBITORS OF THE PROTEASOME BLOCK THE DEGRADATION OF MOST CELL-PROTEINS AND THE GENERATION OF PEPTIDES PRESENTED ON MHC CLASS-I MOLECULES",
      "volume": "78",
      "author": "ROCK K.L.",
      "year": "1994",
      "unstructured": "ROCK K.L., INHIBITORS OF THE PROTEASOME BLOCK THE DEGRADATION OF MOST CELL-PROTEINS AND THE GENERATION OF PEPTIDES PRESENTED ON MHC CLASS-I MOLECULES, CELL 78, 761 (1994).",
      "journal-title": "CELL"
    },
    {
      "key": "e_1_2_1_20_2",
      "author": "SAMBROOK G",
      "year": "1989",
      "unstructured": "SAMBROOK G, MOL CLONING LABORATO (1989).",
      "journal-title": "MOL CLONING LABORATO"
    },
    {
      "key": "e_1_2_1_21_2",
      "doi-asserted-by": "crossref",
      "first-page": "8842",
      "DOI": "10.1073/pnas.90.19.8842",
      "article-title": "IDENTIFICATION OF AN ESSENTIAL GENE, L(3)73AI, WITH A DOMINANT TEMPERATURE-SENSITIVE LETHAL ALLELE, ENCODING A DROSOPHILA PROTEASOME SUBUNIT",
      "volume": "90",
      "author": "SAVILLE K.J.",
      "year": "1993",
      "unstructured": "SAVILLE K.J., IDENTIFICATION OF AN ESSENTIAL GENE, L(3)73AI, WITH A DOMINANT TEMPERATURE-SENSITIVE LETHAL ALLELE, ENCODING A DROSOPHILA PROTEASOME SUBUNIT, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 90, 8842 (1993).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_22_2",
      "volume": "175",
      "author": "SPRADLING A.C.",
      "year": "1986",
      "unstructured": "SPRADLING A.C., DROSOPHILA PRACTICAL 175, (1986).",
      "journal-title": "DROSOPHILA PRACTICAL"
    },
    {
      "key": "e_1_2_1_23_2",
      "first-page": "150",
      "volume": "71",
      "author": "THUMMEL C.S.",
      "year": "1992",
      "unstructured": "THUMMEL C.S., DROS INF SERV 71, 150 (1992).",
      "journal-title": "DROS INF SERV"
    },
    {
      "key": "e_1_2_1_24_2",
      "doi-asserted-by": "crossref",
      "first-page": "543",
      "DOI": "10.1016/0378-1119(88)90177-1",
      "volume": "74",
      "author": "THUMMEL C.S.",
      "year": "1988",
      "unstructured": "THUMMEL C.S., GENE 74, 543 (1988).",
      "journal-title": "GENE"
    },
    {
      "key": "e_1_2_1_25_2",
      "doi-asserted-by": "crossref",
      "first-page": "12021",
      "DOI": "10.1016/S0021-9258(18)99059-9",
      "article-title": "CLONING AND FUNCTIONAL-ANALYSIS OF THE UBIQUITIN-SPECIFIC PROTEASE GENE UBP1 OF SACCHAROMYCES-CEREVISIAE",
      "volume": "266",
      "author": "TOBIAS J.W.",
      "year": "1991",
      "unstructured": "TOBIAS J.W., CLONING AND FUNCTIONAL-ANALYSIS OF THE UBIQUITIN-SPECIFIC PROTEASE GENE UBP1 OF SACCHAROMYCES-CEREVISIAE, JOURNAL OF BIOLOGICAL CHEMISTRY 266, 12021 (1991).",
      "journal-title": "JOURNAL OF BIOLOGICAL CHEMISTRY"
    },
    {
      "key": "e_1_2_1_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1962196"
    },
    {
      "key": "e_1_2_1_27_2",
      "doi-asserted-by": "crossref",
      "first-page": "264",
      "DOI": "10.1016/0012-1606(87)90448-9",
      "article-title": "CELL FATE IN THE DROSOPHILA OMMATIDIUM",
      "volume": "123",
      "author": "TOMLINSON A",
      "year": "1987",
      "unstructured": "TOMLINSON A, CELL FATE IN THE DROSOPHILA OMMATIDIUM, DEVELOPMENTAL BIOLOGY 123, 264 (1987).",
      "journal-title": "DEVELOPMENTAL BIOLOGY"
    },
    {
      "key": "e_1_2_1_28_2",
      "doi-asserted-by": "crossref",
      "first-page": "787",
      "DOI": "10.1016/S0092-8674(94)90502-9",
      "article-title": "UBIQUITIN-DEPENDENT C-JUN DEGRADATION IN-VIVO IS MEDIATED BY THE DELTA-DOMAIN",
      "volume": "78",
      "author": "TREIER M",
      "year": "1994",
      "unstructured": "TREIER M, UBIQUITIN-DEPENDENT C-JUN DEGRADATION IN-VIVO IS MEDIATED BY THE DELTA-DOMAIN, CELL 78, 787 (1994).",
      "journal-title": "CELL"
    },
    {
      "key": "e_1_2_1_29_2",
      "doi-asserted-by": "crossref",
      "first-page": "670",
      "DOI": "10.1126/science.2530630",
      "article-title": "THE NEURON-SPECIFIC PROTEIN PGP-9.5 IS A UBIQUITIN CARBOXYL-TERMINAL HYDROLASE",
      "volume": "246",
      "author": "WILKINSON K.D.",
      "year": "1989",
      "unstructured": "WILKINSON K.D., THE NEURON-SPECIFIC PROTEIN PGP-9.5 IS A UBIQUITIN CARBOXYL-TERMINAL HYDROLASE, SCIENCE 246, 670 (1989).",
      "journal-title": "SCIENCE"
    },
    {
      "key": "e_1_2_1_30_2",
      "first-page": "CH22",
      "volume": "2",
      "author": "WOLFF T",
      "year": "1993",
      "unstructured": "WOLFF T, DEV DROSOPHILA MELAN 2, CH22 (1993).",
      "journal-title": "DEV DROSOPHILA MELAN"
    },
    {
      "key": "e_1_2_1_31_2",
      "doi-asserted-by": "crossref",
      "first-page": "731",
      "DOI": "10.1128/MCB.15.2.731",
      "article-title": "P34(CDC28)-MEDIATED CONTROL OF CLN3 CYCLIN DEGRADATION",
      "volume": "15",
      "author": "YAGLOM J",
      "year": "1995",
      "unstructured": "YAGLOM J, P34(CDC28)-MEDIATED CONTROL OF CLN3 CYCLIN DEGRADATION, MOLECULAR AND CELLULAR BIOLOGY 15, 731 (1995).",
      "journal-title": "MOLECULAR AND CELLULAR BIOLOGY"
    },
    {
      "key": "e_1_2_1_32_2",
      "doi-asserted-by": "crossref",
      "first-page": "214",
      "DOI": "10.1006/dbio.1993.1125",
      "article-title": "CLONING AND ANALYSIS OF EXPRESSION OF A UBIQUITIN CARBOXYL-TERMINAL HYDROLASE EXPRESSED DURING OOGENESIS IN DROSOPHILA-MELANOGASTER",
      "volume": "157",
      "author": "ZHANG N",
      "year": "1993",
      "unstructured": "ZHANG N, CLONING AND ANALYSIS OF EXPRESSION OF A UBIQUITIN CARBOXYL-TERMINAL HYDROLASE EXPRESSED DURING OOGENESIS IN DROSOPHILA-MELANOGASTER, DEVELOPMENTAL BIOLOGY 157, 214 (1993).",
      "journal-title": "DEVELOPMENTAL BIOLOGY"
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.270.5243.1828",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        12
      ]
    ],
    "date-time": "2024-01-12T21:33:48Z",
    "timestamp": 1705095228000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.270.5243.1828"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1995,
        12,
        15
      ]
    ]
  },
  "references-count": 31,
  "journal-issue": {
    "issue": "5243",
    "published-print": {
      "date-parts": [
        [
          1995,
          12,
          15
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.270.5243.1828"
  ],
  "URL": "http://dx.doi.org/10.1126/science.270.5243.1828",
  "relation": {},
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1995,
        12,
        15
      ]
    ]
  }
}