Multiple TAF II s Directing Synergistic Activation of Transcription
10.1126/science.270.5243.1783
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Coordinate activation of transcription by multiple enhancer binding factors is essential for the regulation of pattern formation during development of Drosophila melanogaster . Cell-free transcription reactions are described that recapitulate transcriptional synergism directed by the Drosophila developmental regulators Bicoid (BCD) and Hunchback (HB). Within the basal transcription factor complex TFIID, two specific targets, TAF II 110 and TAF II 60, served as coactivators to mediate transcriptional activation by these two enhancer binding proteins. A quadruple complex containing TATA binding protein (TBP), TAF II 250, TAF II 110, and TAF II 60 mediated transcriptional synergism by BCD and HB, whereas triple TBP-TAF II complexes lacking one or the other target coactivator failed to support synergistic activation. Deoxyribonuclease I footprint protection experiments revealed that an integral step leading to transcriptional synergism involves the recruitment of TBP-TAF II complexes to the promoter by way of multivalent contacts between activators and selected TAF II s. Thus, the concerted action of multiple regulators with different coactivators helps to establish the pattern and level of segmentation gene transcription during Drosophila development.

Bibliography

Sauer, F., Hansen, S. K., & Tjian, R. (1995). Multiple TAF II s Directing Synergistic Activation of Transcription. Science, 270(5243), 1783–1788.

Authors 3
  1. Frank Sauer (first)
  2. Stig K. Hansen (additional)
  3. Robert Tjian (additional)
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Dates
Type When
Created 18 years, 10 months ago (Oct. 27, 2006, 2:19 p.m.)
Deposited 1 year, 7 months ago (Jan. 12, 2024, 4:35 p.m.)
Indexed 3 weeks, 3 days ago (Aug. 5, 2025, 8:39 a.m.)
Issued 29 years, 8 months ago (Dec. 15, 1995)
Published 29 years, 8 months ago (Dec. 15, 1995)
Published Print 29 years, 8 months ago (Dec. 15, 1995)
Funders 0

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@article{Sauer_1995, title={Multiple TAF II s Directing Synergistic Activation of Transcription}, volume={270}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.270.5243.1783}, DOI={10.1126/science.270.5243.1783}, number={5243}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Sauer, Frank and Hansen, Stig K. and Tjian, Robert}, year={1995}, month=dec, pages={1783–1788} }