Activation of the Estrogen Receptor Through Phosphorylation by Mitogen-Activated Protein Kinase
10.1126/science.270.5241.1491
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

The phosphorylation of the human estrogen receptor (ER) serine residue at position 118 is required for full activity of the ER activation function 1 (AF-1). This Ser 118 is phosphorylated by mitogen-activated protein kinase (MAPK) in vitro and in cells treated with epidermal growth factor (EGF) and insulin-like growth factor (IGF) in vivo. Overexpression of MAPK kinase (MAPKK) or of the guanine nucleotide binding protein Ras, both of which activate MAPK, enhanced estrogen-induced and antiestrogen (tamoxifen)-induced transcriptional activity of wild-type ER, but not that of a mutant ER with an alanine in place of Ser 118 . Thus, the activity of the amino-terminal AF-1 of the ER is modulated by the phosphorylation of Ser 118 through the Ras-MAPK cascade of the growth factor signaling pathways.

Bibliography

Kato, S., Endoh, H., Masuhiro, Y., Kitamoto, T., Uchiyama, S., Sasaki, H., Masushige, S., Gotoh, Y., Nishida, E., Kawashima, H., Metzger, D., & Chambon, P. (1995). Activation of the Estrogen Receptor Through Phosphorylation by Mitogen-Activated Protein Kinase. Science, 270(5241), 1491–1494.

Authors 12
  1. Shigeaki Kato (first)
  2. Hideki Endoh (additional)
  3. Yoshikazu Masuhiro (additional)
  4. Takuya Kitamoto (additional)
  5. Shimami Uchiyama (additional)
  6. Haruna Sasaki (additional)
  7. Shoichi Masushige (additional)
  8. Yukiko Gotoh (additional)
  9. Eisuke Nishida (additional)
  10. Hiroyuki Kawashima (additional)
  11. Daniel Metzger (additional)
  12. Pierre Chambon (additional)
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Dates
Type When
Created 18 years, 10 months ago (Oct. 27, 2006, 2:19 p.m.)
Deposited 1 year, 7 months ago (Jan. 12, 2024, 4:37 p.m.)
Indexed 17 hours, 12 minutes ago (Aug. 30, 2025, 12:43 p.m.)
Issued 29 years, 8 months ago (Dec. 1, 1995)
Published 29 years, 8 months ago (Dec. 1, 1995)
Published Print 29 years, 8 months ago (Dec. 1, 1995)
Funders 0

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@article{Kato_1995, title={Activation of the Estrogen Receptor Through Phosphorylation by Mitogen-Activated Protein Kinase}, volume={270}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.270.5241.1491}, DOI={10.1126/science.270.5241.1491}, number={5241}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Kato, Shigeaki and Endoh, Hideki and Masuhiro, Yoshikazu and Kitamoto, Takuya and Uchiyama, Shimami and Sasaki, Haruna and Masushige, Shoichi and Gotoh, Yukiko and Nishida, Eisuke and Kawashima, Hiroyuki and Metzger, Daniel and Chambon, Pierre}, year={1995}, month=dec, pages={1491–1494} }