DOI: 10.1126/science.2675315. Electrospray Ionization for Mass Spectrometry of Large Biomolecules

Electrospray Ionization for Mass Spectrometry of Large Biomolecules

10.1126/science.2675315

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Electrospray ionization has recently emerged as a powerful technique for producing intact ions in vacuo from large and complex species in solution. To an extent greater than has previously been possible with the more familiar "soft" ionization methods, this technique makes the power and elegance of mass spectrometric analysis applicable to the large and fragile polar molecules that play such vital roles in biological systems. The distinguishing features of electrospray spectra for large molecules are coherent sequences of peaks whose component ions are multiply charged, the ions of each peak differing by one charge from those of adjacent neighbors in the sequence. Spectra have been obtained for biopolymers including oligonucleotides and proteins, the latter having molecular weights up to 130,000, with as yet no evidence of an upper limit.

Bibliography

Fenn, J. B., Mann, M., Meng, C. K., Wong, S. F., & Whitehouse, C. M. (1989). Electrospray Ionization for Mass Spectrometry of Large Biomolecules. Science, 246(4926), 64â71.

Authors 5

John B. Fenn (first)
Matthias Mann (additional)
Chin Kai Meng (additional)
Shek Fu Wong (additional)
Craig M. Whitehouse (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 4:39 p.m.)
Indexed	36 minutes ago (Aug. 26, 2025, 2:30 a.m.)
Issued	35 years, 10 months ago (Oct. 6, 1989)
Published	35 years, 10 months ago (Oct. 6, 1989)
Published Print	35 years, 10 months ago (Oct. 6, 1989)

Funders 0

None

BibTeX

@article{Fenn_1989, title={Electrospray Ionization for Mass Spectrometry of Large Biomolecules}, volume={246}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2675315}, DOI={10.1126/science.2675315}, number={4926}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Fenn, John B. and Mann, Matthias and Meng, Chin Kai and Wong, Shek Fu and Whitehouse, Craig M.}, year={1989}, month=oct, pages={64–71} }

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