DOI: 10.1126/science.267.5204.1635. Lipid Tubule Self-Assembly: Length Dependence on Cooling Rate Through a First-Order Phase Transition

Lipid Tubule Self-Assembly: Length Dependence on Cooling Rate Through a First-Order Phase Transition

10.1126/science.267.5204.1635

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The formation kinetics and self-assembly of multilamellar tubules of the diacetylenic phospholipid 1,2-bis(tricosa-10,12-diynoyl)- sn -glycerol-3-phosphocholine formed under controlled cooling rates were studied by x-ray diffraction and optical, atomic force, and scanning electron microscopy. Tubule formation was driven by a reversible first-order phase transition from an intralamellar, chain-melted L α phase to a chain-frozen L β , phase. These observations are the basis of a highly efficient method of tubule production in which tubule lengths can be controlled, between 1 and 100 micrometers, by varying the cooling rate. These tubules can be made in suspensions with 10 percent lipid by mass, far exceeding the lipid solubility limit.

Bibliography

Thomas, B. N., Safinya, C. R., Plano, R. J., & Clark, N. A. (1995). Lipid Tubule Self-Assembly: Length Dependence on Cooling Rate Through a First-Order Phase Transition. Science, 267(5204), 1635â1638.

Authors 4

Britt N. Thomas (first)
Cyrus R. Safinya (additional)
Robert J. Plano (additional)
Noel A. Clark (additional)

References 23 Referenced 178

ARCHIBALD, D.D., TEMPLATE MINERALIZATION OF SELF-ASSEMBLED ANISOTROPIC LIPID MICROSTRUCTURES, NATURE 364: 430 (1993). (10.1038/364430a0) / NATURE (1993)
CAFFREY, M, DIACETYLENIC LIPID MICROSTRUCTURES - STRUCTURAL CHARACTERIZATION BY X-RAY-DIFFRACTION AND COMPARISON WITH THE SATURATED PHOSPHATIDYLCHOLINE ANALOG, BIOCHEMISTRY 30: 2134 (1991). (10.1021/bi00222a018) / BIOCHEMISTRY (1991)
DAGANI, R, CHEMICAL & ENGINEERING NEWS 71: 19 (1993). (10.1021/cen-v071n034.p019) / CHEMICAL & ENGINEERING NEWS (1993)
DEGENNES, P.G., ELECTROSTATIC BUCKLING OF CHIRAL LIPID BILAYERS, COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE II 304: 259 (1987). / COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE II (1987)
HELFRICH, W, INTRINSIC BENDING FORCE IN ANISOTROPIC MEMBRANES MADE OF CHIRAL MOLECULES, PHYSICAL REVIEW A 38: 3065 (1988). (10.1103/PhysRevA.38.3065) / PHYSICAL REVIEW A (1988)
JANIAK, M.J., TEMPERATURE AND COMPOSITIONAL DEPENDENCE OF THE STRUCTURE OF HYDRATED DIMYRISTOYL LECITHIN, JOURNAL OF BIOLOGICAL CHEMISTRY 254: 6068 (1979). (10.1016/S0021-9258(18)50520-2) / JOURNAL OF BIOLOGICAL CHEMISTRY (1979)
LUBENSKY, T.C., ORIENTATIONAL ORDER AND VESICLE SHAPE, JOURNAL DE PHYSIQUE II 2: 371 (1992). (10.1051/jp2:1992133) / JOURNAL DE PHYSIQUE II (1992)
LUZZATI, V, BIOLOGICAL MEMBRANES 1: 71 (1968). / BIOLOGICAL MEMBRANES (1968)
NIR, S, AGGREGATION AND FUSION OF PHOSPHOLIPID-VESICLES, PROGRESS IN SURFACE SCIENCE 13: 1 (1983). (10.1016/0079-6816(83)90010-2) / PROGRESS IN SURFACE SCIENCE (1983)
OUYANG, Z.C., PHYS REV A 43: 6826 (1991). (10.1103/PhysRevA.43.6826) / PHYS REV A (1991)
OUYANG, Z.C., HELICAL STRUCTURES OF TILTED CHIRAL LIPID BILAYERS VIEWED AS CHOLESTERIC LIQUID-CRYSTALS, PHYSICAL REVIEW LETTERS 65: 1679 (1990). (10.1103/PhysRevLett.65.1679) / PHYSICAL REVIEW LETTERS (1990)
RATNA, B.R., EFFECT OF ALCOHOL CHAIN-LENGTH ON TUBULE FORMATION IN 1,2-BIS(10,12-TRICOSADIYNOYL)-SN-GLYCERO-3-PHOSPHOCHOLINE, CHEMISTRY AND PHYSICS OF LIPIDS 63: 47 (1992). (10.1016/0009-3084(92)90021-G) / CHEMISTRY AND PHYSICS OF LIPIDS (1992)
ROUX, D, A SYNCHROTRON X-RAY STUDY OF COMPETING UNDULATION AND ELECTROSTATIC INTERLAYER INTERACTIONS IN FLUID MULTIMEMBRANE LYOTROPIC PHASES, JOURNAL DE PHYSIQUE 49: 307 (1988). (10.1051/jphys:01988004902030700) / JOURNAL DE PHYSIQUE (1988)
SAFINYA, C.R., STERIC INTERACTIONS IN A MODEL MULTIMEMBRANE SYSTEM - A SYNCHROTRON X-RAY STUDY, PHYSICAL REVIEW LETTERS 57: 2718 (1986). (10.1103/PhysRevLett.57.2718) / PHYSICAL REVIEW LETTERS (1986)
10.1126/science.262.5140.1669
SELINGER, J.V., THEORY OF CHIRAL LIPID TUBULES, PHYSICAL REVIEW LETTERS 71: 4091 (1993). (10.1103/PhysRevLett.71.4091) / PHYSICAL REVIEW LETTERS (1993)
10.1126/science.242.4884.1406
SMITH, G.S., X-RAY STRUCTURAL STUDIES OF FREELY SUSPENDED ORDERED HYDRATED DMPC MULTIMEMBRANE FILMS, JOURNAL OF CHEMICAL PHYSICS 92: 4519 (1990). (10.1063/1.457764) / JOURNAL OF CHEMICAL PHYSICS (1990)
SMITH, G.S., STRUCTURE OF THE L-BETA PHASES IN A HYDRATED PHOSPHATIDYLCHOLINE MULTIMEMBRANE, PHYSICAL REVIEW LETTERS 60: 813 (1988). (10.1103/PhysRevLett.60.813) / PHYSICAL REVIEW LETTERS (1988)
TARDIEU, A, STRUCTURE AND POLYMORPHISM OF HYDROCARBON CHAINS OF LIPIDS - STUDY OF LECITHIN-WATER PHASES, JOURNAL OF MOLECULAR BIOLOGY 75: 711 (1973). (10.1016/0022-2836(73)90303-3) / JOURNAL OF MOLECULAR BIOLOGY (1973)
THOMAS, B, MATERIALS RESEARCH SOCIETY SYMPOSIA PROCEEDINGS 248: 83 (1992). / MATERIALS RESEARCH SOCIETY SYMPOSIA PROCEEDINGS (1992)
YAGER, P, STRUCTURE OF LIPID TUBULES FORMED FROM A POLYMERIZABLE LECITHIN, BIOPHYSICAL JOURNAL 48: 899 (1985). (10.1016/S0006-3495(85)83852-2) / BIOPHYSICAL JOURNAL (1985)
YAGER, P, FORMATION OF TUBULES BY A POLYMERIZABLE SURFACTANT, MOLECULAR CRYSTALS AND LIQUID CRYSTALS 106: 371 (1984). (10.1080/00268948408071454) / MOLECULAR CRYSTALS AND LIQUID CRYSTALS (1984)

Dates

Type	When
Created	18 years, 9 months ago (Oct. 27, 2006, 2:19 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 6:03 p.m.)
Indexed	1 month, 3 weeks ago (June 30, 2025, 7:27 a.m.)
Issued	30 years, 5 months ago (March 17, 1995)
Published	30 years, 5 months ago (March 17, 1995)
Published Print	30 years, 5 months ago (March 17, 1995)

Funders 0

None

BibTeX

@article{Thomas_1995, title={Lipid Tubule Self-Assembly: Length Dependence on Cooling Rate Through a First-Order Phase Transition}, volume={267}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.267.5204.1635}, DOI={10.1126/science.267.5204.1635}, number={5204}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Thomas, Britt N. and Safinya, Cyrus R. and Plano, Robert J. and Clark, Noel A.}, year={1995}, month=mar, pages={1635–1638} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        6,
        30
      ]
    ],
    "date-time": "2025-06-30T11:27:21Z",
    "timestamp": 1751282841131
  },
  "reference-count": 23,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "5204",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1995,
        3,
        17
      ]
    ]
  },
  "abstract": "<jats:p>\n            The formation kinetics and self-assembly of multilamellar tubules of the diacetylenic phospholipid 1,2-bis(tricosa-10,12-diynoyl)-\n            <jats:italic>sn</jats:italic>\n            -glycerol-3-phosphocholine formed under controlled cooling rates were studied by x-ray diffraction and optical, atomic force, and scanning electron microscopy. Tubule formation was driven by a reversible first-order phase transition from an intralamellar, chain-melted L\n            <jats:sub>\u03b1</jats:sub>\n            phase to a chain-frozen L\n            <jats:sub>\u03b2</jats:sub>\n            , phase. These observations are the basis of a highly efficient method of tubule production in which tubule lengths can be controlled, between 1 and 100 micrometers, by varying the cooling rate. These tubules can be made in suspensions with 10 percent lipid by mass, far exceeding the lipid solubility limit.\n          </jats:p>",
  "DOI": "10.1126/science.267.5204.1635",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        10,
        27
      ]
    ],
    "date-time": "2006-10-27T18:19:42Z",
    "timestamp": 1161973182000
  },
  "page": "1635-1638",
  "source": "Crossref",
  "is-referenced-by-count": 178,
  "title": "Lipid Tubule Self-Assembly: Length Dependence on Cooling Rate Through a First-Order Phase Transition",
  "prefix": "10.1126",
  "volume": "267",
  "author": [
    {
      "given": "Britt N.",
      "family": "Thomas",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Condensed Matter Laboratory, Department of Physics, University of Colorado, Boulder, CO 80309, and Exxon Research and Engineering Company, Annandale, NJ 08801, USA."
        }
      ]
    },
    {
      "given": "Cyrus R.",
      "family": "Safinya",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Materials and Physics Departments and the Interdepartmental Program on Biochemistry and Molecular Biology, University of California, Santa Barbara, CA 93106, USA."
        }
      ]
    },
    {
      "given": "Robert J.",
      "family": "Plano",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Exxon Research and Engineering Company, Annandale, NJ 08801, USA."
        }
      ]
    },
    {
      "given": "Noel A.",
      "family": "Clark",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Condensed Matter Laboratory, Department of Physics, University of Colorado, Boulder, CO 80309, USA."
        }
      ]
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_2_1_1_1",
      "doi-asserted-by": "crossref",
      "first-page": "430",
      "DOI": "10.1038/364430a0",
      "volume": "364",
      "year": "1993",
      "unstructured": "ARCHIBALD, D.D., TEMPLATE MINERALIZATION OF SELF-ASSEMBLED ANISOTROPIC LIPID MICROSTRUCTURES, NATURE 364: 430 (1993).",
      "journal-title": "NATURE"
    },
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "crossref",
      "first-page": "2134",
      "DOI": "10.1021/bi00222a018",
      "volume": "30",
      "year": "1991",
      "unstructured": "CAFFREY, M, DIACETYLENIC LIPID MICROSTRUCTURES - STRUCTURAL CHARACTERIZATION BY X-RAY-DIFFRACTION AND COMPARISON WITH THE SATURATED PHOSPHATIDYLCHOLINE ANALOG, BIOCHEMISTRY 30: 2134 (1991).",
      "journal-title": "BIOCHEMISTRY"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "crossref",
      "first-page": "19",
      "DOI": "10.1021/cen-v071n034.p019",
      "volume": "71",
      "year": "1993",
      "unstructured": "DAGANI, R, CHEMICAL & ENGINEERING NEWS 71: 19 (1993).",
      "journal-title": "CHEMICAL & ENGINEERING NEWS"
    },
    {
      "key": "e_1_2_1_4_1",
      "first-page": "259",
      "volume": "304",
      "year": "1987",
      "unstructured": "DEGENNES, P.G., ELECTROSTATIC BUCKLING OF CHIRAL LIPID BILAYERS, COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE II 304: 259 (1987).",
      "journal-title": "COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE II"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "crossref",
      "first-page": "3065",
      "DOI": "10.1103/PhysRevA.38.3065",
      "volume": "38",
      "year": "1988",
      "unstructured": "HELFRICH, W, INTRINSIC BENDING FORCE IN ANISOTROPIC MEMBRANES MADE OF CHIRAL MOLECULES, PHYSICAL REVIEW A 38: 3065 (1988).",
      "journal-title": "PHYSICAL REVIEW A"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "crossref",
      "first-page": "6068",
      "DOI": "10.1016/S0021-9258(18)50520-2",
      "volume": "254",
      "year": "1979",
      "unstructured": "JANIAK, M.J., TEMPERATURE AND COMPOSITIONAL DEPENDENCE OF THE STRUCTURE OF HYDRATED DIMYRISTOYL LECITHIN, JOURNAL OF BIOLOGICAL CHEMISTRY 254: 6068 (1979).",
      "journal-title": "JOURNAL OF BIOLOGICAL CHEMISTRY"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "crossref",
      "first-page": "371",
      "DOI": "10.1051/jp2:1992133",
      "volume": "2",
      "year": "1992",
      "unstructured": "LUBENSKY, T.C., ORIENTATIONAL ORDER AND VESICLE SHAPE, JOURNAL DE PHYSIQUE II 2: 371 (1992).",
      "journal-title": "JOURNAL DE PHYSIQUE II"
    },
    {
      "key": "e_1_2_1_8_1",
      "first-page": "71",
      "volume": "1",
      "year": "1968",
      "unstructured": "LUZZATI, V, BIOLOGICAL MEMBRANES 1: 71 (1968).",
      "journal-title": "BIOLOGICAL MEMBRANES"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1016/0079-6816(83)90010-2",
      "volume": "13",
      "year": "1983",
      "unstructured": "NIR, S, AGGREGATION AND FUSION OF PHOSPHOLIPID-VESICLES, PROGRESS IN SURFACE SCIENCE 13: 1 (1983).",
      "journal-title": "PROGRESS IN SURFACE SCIENCE"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "crossref",
      "first-page": "6826",
      "DOI": "10.1103/PhysRevA.43.6826",
      "volume": "43",
      "year": "1991",
      "unstructured": "OUYANG, Z.C., PHYS REV A 43: 6826 (1991).",
      "journal-title": "PHYS REV A"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "crossref",
      "first-page": "1679",
      "DOI": "10.1103/PhysRevLett.65.1679",
      "volume": "65",
      "year": "1990",
      "unstructured": "OUYANG, Z.C., HELICAL STRUCTURES OF TILTED CHIRAL LIPID BILAYERS VIEWED AS CHOLESTERIC LIQUID-CRYSTALS, PHYSICAL REVIEW LETTERS 65: 1679 (1990).",
      "journal-title": "PHYSICAL REVIEW LETTERS"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "crossref",
      "first-page": "47",
      "DOI": "10.1016/0009-3084(92)90021-G",
      "volume": "63",
      "year": "1992",
      "unstructured": "RATNA, B.R., EFFECT OF ALCOHOL CHAIN-LENGTH ON TUBULE FORMATION IN 1,2-BIS(10,12-TRICOSADIYNOYL)-SN-GLYCERO-3-PHOSPHOCHOLINE, CHEMISTRY AND PHYSICS OF LIPIDS 63: 47 (1992).",
      "journal-title": "CHEMISTRY AND PHYSICS OF LIPIDS"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "crossref",
      "first-page": "307",
      "DOI": "10.1051/jphys:01988004902030700",
      "volume": "49",
      "year": "1988",
      "unstructured": "ROUX, D, A SYNCHROTRON X-RAY STUDY OF COMPETING UNDULATION AND ELECTROSTATIC INTERLAYER INTERACTIONS IN FLUID MULTIMEMBRANE LYOTROPIC PHASES, JOURNAL DE PHYSIQUE 49: 307 (1988).",
      "journal-title": "JOURNAL DE PHYSIQUE"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "crossref",
      "first-page": "2718",
      "DOI": "10.1103/PhysRevLett.57.2718",
      "volume": "57",
      "year": "1986",
      "unstructured": "SAFINYA, C.R., STERIC INTERACTIONS IN A MODEL MULTIMEMBRANE SYSTEM - A SYNCHROTRON X-RAY STUDY, PHYSICAL REVIEW LETTERS 57: 2718 (1986).",
      "journal-title": "PHYSICAL REVIEW LETTERS"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.262.5140.1669"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "crossref",
      "first-page": "4091",
      "DOI": "10.1103/PhysRevLett.71.4091",
      "volume": "71",
      "year": "1993",
      "unstructured": "SELINGER, J.V., THEORY OF CHIRAL LIPID TUBULES, PHYSICAL REVIEW LETTERS 71: 4091 (1993).",
      "journal-title": "PHYSICAL REVIEW LETTERS"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.242.4884.1406"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "crossref",
      "first-page": "4519",
      "DOI": "10.1063/1.457764",
      "volume": "92",
      "year": "1990",
      "unstructured": "SMITH, G.S., X-RAY STRUCTURAL STUDIES OF FREELY SUSPENDED ORDERED HYDRATED DMPC MULTIMEMBRANE FILMS, JOURNAL OF CHEMICAL PHYSICS 92: 4519 (1990).",
      "journal-title": "JOURNAL OF CHEMICAL PHYSICS"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "crossref",
      "first-page": "813",
      "DOI": "10.1103/PhysRevLett.60.813",
      "volume": "60",
      "year": "1988",
      "unstructured": "SMITH, G.S., STRUCTURE OF THE L-BETA PHASES IN A HYDRATED PHOSPHATIDYLCHOLINE MULTIMEMBRANE, PHYSICAL REVIEW LETTERS 60: 813 (1988).",
      "journal-title": "PHYSICAL REVIEW LETTERS"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "crossref",
      "first-page": "711",
      "DOI": "10.1016/0022-2836(73)90303-3",
      "volume": "75",
      "year": "1973",
      "unstructured": "TARDIEU, A, STRUCTURE AND POLYMORPHISM OF HYDROCARBON CHAINS OF LIPIDS - STUDY OF LECITHIN-WATER PHASES, JOURNAL OF MOLECULAR BIOLOGY 75: 711 (1973).",
      "journal-title": "JOURNAL OF MOLECULAR BIOLOGY"
    },
    {
      "key": "e_1_2_1_21_1",
      "first-page": "83",
      "volume": "248",
      "year": "1992",
      "unstructured": "THOMAS, B, MATERIALS RESEARCH SOCIETY SYMPOSIA PROCEEDINGS 248: 83 (1992).",
      "journal-title": "MATERIALS RESEARCH SOCIETY SYMPOSIA PROCEEDINGS"
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "crossref",
      "first-page": "899",
      "DOI": "10.1016/S0006-3495(85)83852-2",
      "volume": "48",
      "year": "1985",
      "unstructured": "YAGER, P, STRUCTURE OF LIPID TUBULES FORMED FROM A POLYMERIZABLE LECITHIN, BIOPHYSICAL JOURNAL 48: 899 (1985).",
      "journal-title": "BIOPHYSICAL JOURNAL"
    },
    {
      "key": "e_1_2_1_23_1",
      "doi-asserted-by": "crossref",
      "first-page": "371",
      "DOI": "10.1080/00268948408071454",
      "volume": "106",
      "year": "1984",
      "unstructured": "YAGER, P, FORMATION OF TUBULES BY A POLYMERIZABLE SURFACTANT, MOLECULAR CRYSTALS AND LIQUID CRYSTALS 106: 371 (1984).",
      "journal-title": "MOLECULAR CRYSTALS AND LIQUID CRYSTALS"
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.267.5204.1635",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        12
      ]
    ],
    "date-time": "2024-01-12T23:03:06Z",
    "timestamp": 1705100586000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.267.5204.1635"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1995,
        3,
        17
      ]
    ]
  },
  "references-count": 23,
  "journal-issue": {
    "issue": "5204",
    "published-print": {
      "date-parts": [
        [
          1995,
          3,
          17
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.267.5204.1635"
  ],
  "URL": "http://dx.doi.org/10.1126/science.267.5204.1635",
  "relation": {},
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1995,
        3,
        17
      ]
    ]
  }
}