DOI: 10.1126/science.2660260. Altering the Genome by Homologous Recombination

Altering the Genome by Homologous Recombination

10.1126/science.2660260

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Homologous recombination between DNA sequences residing in the chromosome and newly introduced, cloned DNA sequences (gene targeting) allows the transfer of any modification of the cloned gene into the genome of a living cell. This article discusses the current status of gene targeting with particular emphasis on germ line modification of the mouse genome, and describes the different methods so far employed to identify those rare embryonic stem cells in which the desired targeting event has occurred.

Bibliography

Capecchi, M. R. (1989). Altering the Genome by Homologous Recombination. Science, 244(4910), 1288â1292.

Authors 1

Mario R. Capecchi (first)

References 34 Referenced 1,554

CAPECCHI, M.R., HIGH-EFFICIENCY TRANSFORMATION BY DIRECT MICRO-INJECTION OF DNA INTO CULTURED MAMMALIAN-CELLS, CELL 22: 479 (1980). (10.1016/0092-8674(80)90358-X) / CELL (1980)
CAPECCHI, M.R., THE NEW MOUSE GENETICS - ALTERING THE GENOME BY GENE TARGETING, TRENDS IN GENETICS 5: 70 (1989). (10.1016/0168-9525(89)90029-2) / TRENDS IN GENETICS (1989)
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DOETSCHMAN, T, TARGETED CORRECTION OF A MUTANT HPRT GENE IN MOUSE EMBRYONIC STEM-CELLS, NATURE 330: 576 (1987). (10.1038/330576a0) / NATURE (1987)
DOETSCHMAN, T, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 8553 (1988). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1988)
DRESSLER, G.R., DO MULTIGENE FAMILIES REGULATE VERTEBRATE DEVELOPMENT, TRENDS IN GENETICS 4: 214 (1988). (10.1016/S0168-9525(88)80003-9) / TRENDS IN GENETICS (1988)
EVANS, M.J., ESTABLISHMENT IN CULTURE OF PLURIPOTENTIAL CELLS FROM MOUSE EMBRYOS, NATURE 292: 154 (1981). (10.1038/292154a0) / NATURE (1981)
FOLGER, K, ANALYSIS OF HOMOLOGOUS RECOMBINATION IN CULTURED MAMMALIAN-CELLS, COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY 49: 123 (1984). (10.1101/SQB.1984.049.01.016) / COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY (1984)
FOLGER, K.R., PATTERNS OF INTEGRATION OF DNA MICRO-INJECTED INTO CULTURED MAMMALIAN-CELLS - EVIDENCE FOR HOMOLOGOUS RECOMBINATION BETWEEN INJECTED PLASMID DNA-MOLECULES, MOLECULAR AND CELLULAR BIOLOGY 2: 1372 (1982). / MOLECULAR AND CELLULAR BIOLOGY (1982)
10.1073/pnas.83.23.9065
HOLLAND, PWH, EXPRESSION OF HOMEO BOX GENES DURING MOUSE DEVELOPMENT - A REVIEW, GENES & DEVELOPMENT 2: 773 (1988). (10.1101/gad.2.7.773) / GENES & DEVELOPMENT (1988)
HOOPER, M, HPRT-DEFICIENT (LESCH-NYHAN) MOUSE EMBRYOS DERIVED FROM GERMLINE COLONIZATION BY CULTURED-CELLS, NATURE 326: 292 (1987). (10.1038/326292a0) / NATURE (1987)
INGHAM, P.W., THE MOLECULAR-GENETICS OF EMBRYONIC PATTERN-FORMATION IN DROSOPHILA, NATURE 335: 25 (1988). (10.1038/335025a0) / NATURE (1988)
JASIN, M, HOMOLOGOUS INTEGRATION IN MAMMALIAN-CELLS WITHOUT TARGET GENE SELECTION, GENES & DEVELOPMENT 2: 1353 (1988). (10.1101/gad.2.11.1353) / GENES & DEVELOPMENT (1988)
JOYNER, A.L., PRODUCTION OF A MUTATION IN MOUSE EN-2 GENE BY HOMOLOGOUS RECOMBINATION IN EMBRYONIC STEM-CELLS, NATURE 338: 153 (1989). (10.1038/338153a0) / NATURE (1989)
KEUHN, M.R., NATURE 326: 295 (1987). (10.1038/326295a0) / NATURE (1987)
KIM, H.S., RECOMBINANT FRAGMENT ASSAY FOR GENE TARGETTING BASED ON THE POLYMERASE CHAIN-REACTION, NUCLEIC ACIDS RESEARCH 16: 8887 (1988). (10.1093/nar/16.18.8887) / NUCLEIC ACIDS RESEARCH (1988)
KOSTIC D unpublished data.
LETSOU, A, EFFECT OF THE MOLECULAR NATURE OF MUTATION ON THE EFFICIENCY OF INTRACHROMOSOMAL GENE CONVERSION IN MOUSE CELLS, GENETICS 117: 759 (1987). (10.1093/genetics/117.4.759) / GENETICS (1987)
LIN, F.L., HOMOLOGOUS RECOMBINATION IN MOUSE L-CELLS, COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY 49: 139 (1984). (10.1101/SQB.1984.049.01.017) / COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY (1984)
MANSOUR, S.L., DISRUPTION OF THE PROTO-ONCOGENE INT-2 IN MOUSE EMBRYO-DERIVED STEM-CELLS - A GENERAL STRATEGY FOR TARGETING MUTATIONS TO NON-SELECTABLE GENES, NATURE 336: 348 (1988). (10.1038/336348a0) / NATURE (1988)
Martin, G. R., Proceedings of the National Academy of Sciences of the United States of America 79: 7634 (1982). / Proceedings of the National Academy of Sciences of the United States of America (1982)
MULLIS, K.B., SPECIFIC SYNTHESIS OF DNA INVITRO VIA A POLYMERASE-CATALYZED CHAIN-REACTION, METHODS IN ENZYMOLOGY 155: 335 (1987). (10.1016/0076-6879(87)55023-6) / METHODS IN ENZYMOLOGY (1987)
PASZKOWSKI, J, GENE TARGETING IN PLANTS, EMBO JOURNAL 7: 4021 (1988). (10.1002/j.1460-2075.1988.tb03295.x) / EMBO JOURNAL (1988)
ROBERTSON, E, GERM-LINE TRANSMISSION OF GENES INTRODUCED INTO CULTURED PLURIPOTENTIAL CELLS BY RETROVIRAL VECTOR, NATURE 323: 445 (1986). (10.1038/323445a0) / NATURE (1986)
ROTH, D.B., MECHANISMS OF NONHOMOLOGOUS RECOMBINATION IN MAMMALIAN-CELLS, MOLECULAR AND CELLULAR BIOLOGY 5: 2599 (1985). / MOLECULAR AND CELLULAR BIOLOGY (1985)
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SMITH, AJH, HOMOLOGOUS RECOMBINATION BETWEEN DEFECTIVE NEO GENES IN MOUSE 3T6 CELLS, COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY 49: 171 (1984). (10.1101/SQB.1984.049.01.020) / COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY (1984)
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ZIMMER, A, PRODUCTION OF CHIMAERIC MICE CONTAINING EMBRYONIC STEM (ES) CELLS CARRYING A HOMEOBOX HOX-1.1 ALLELE MUTATED BY HOMOLOGOUS RECOMBINATION, NATURE 338: 150 (1989). (10.1038/338150a0) / NATURE (1989)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 5:36 p.m.)
Indexed	22 hours, 32 minutes ago (Aug. 24, 2025, 6:54 p.m.)
Issued	36 years, 2 months ago (June 16, 1989)
Published	36 years, 2 months ago (June 16, 1989)
Published Print	36 years, 2 months ago (June 16, 1989)

Funders 0

None

BibTeX

@article{Capecchi_1989, title={Altering the Genome by Homologous Recombination}, volume={244}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2660260}, DOI={10.1126/science.2660260}, number={4910}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Capecchi, Mario R.}, year={1989}, month=jun, pages={1288–1292} }

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