DOI: 10.1126/science.2658054. Induction of Mesoderm by a Viral Oncogene in Early <i>Xenopus</i> Embryos

Induction of Mesoderm by a Viral Oncogene in Early Xenopus Embryos

10.1126/science.2658054

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

During frog embryogenesis, mesoderm is specified in the equatorial region of the early embryo by a signal from the vegetal hemisphere. Prospective ectodermal cells dissected from the animal hemisphere can be respecified to form mesodermal tissues by recombination with vegetal tissue or by treatment with any of several polypeptide growth factors or growth factor-like molecules. Together with the discovery that several developmental mutations in Drosophila are in genes with significant homology to mammalian mitogens and oncogenes, these observations suggest that early developmental signals may use similar transduction pathways to mitogenic signals characterized in cultured mammalian cells. Whether mesoderm can be induced by activation of intracellular signal transduction pathways implicated in mitogenesis and oncogenesis has been investigated with the viral oncogene polyoma middle T. Microinjection of middle T messenger RNA into early embryos results in the respecification of isolated prospective ectodermal tissue to form characteristic mesodermal structures. Middle T in frog blastomeres appears to associate with cellular activities similar to those observed in polyoma-transformed mouse cells, and transformation-defective middle T mutants fail to induce mesoderm. These results suggest that early inductive signals and mitogenic and oncogenic stimuli may share common signal transduction pathways.

Bibliography

Whitman, M., & Melton, D. A. (1989). Induction of Mesoderm by a Viral Oncogene in Early Xenopus Embryos. Science, 244(4906), 803â806.

Authors 2

Malcolm Whitman (first)
D. A. Melton (additional)

References 30 Referenced 31

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BOLEN, J.B., ENHANCEMENT OF CELLULAR SRC GENE-PRODUCT ASSOCIATED TYROSYL KINASE-ACTIVITY FOLLOWING POLYOMA-VIRUS INFECTION AND TRANSFORMATION, CELL 38: 767 (1984). (10.1016/0092-8674(84)90272-1) / CELL (1984)
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KAPLAN, D.R., COMMON ELEMENTS IN GROWTH-FACTOR STIMULATION AND ONCOGENIC TRANSFORMATION - 85-KD PHOSPHOPROTEIN AND PHOSPHATIDYLINOSITOL KINASE-ACTIVITY, CELL 50: 1021 (1987). (10.1016/0092-8674(87)90168-1) / CELL (1987)
KIMELMAN, D, SYNERGISTIC INDUCTION OF MESODERM BY FGF AND TGF-BETA AND THE IDENTIFICATION OF AN MESSENGER-RNA CODING FOR FGF IN THE EARLY XENOPUS EMBRYO, CELL 51: 869 (1987). (10.1016/0092-8674(87)90110-3) / CELL (1987)
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KORNBLUTH, S, ASSOCIATION OF THE POLYOMAVIRUS MIDDLE-T ANTIGEN WITH C-YES PROTEIN, NATURE 325: 171 (1987). (10.1038/325171a0) / NATURE (1987)
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PASQUALE, E.B., COMPARATIVE-STUDY OF THE TYROSINE PHOSPHORYLATION OF PROTEINS IN SWISS 3T3 FIBROBLASTS STIMULATED BY A VARIETY OF MITOGENIC AGENTS, JOURNAL OF CELLULAR PHYSIOLOGY 137: 146 (1988). (10.1002/jcp.1041370118) / JOURNAL OF CELLULAR PHYSIOLOGY (1988)
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SMITH, J.C., A MESODERM-INDUCING FACTOR IS PRODUCED BY A XENOPUS CELL-LINE, DEVELOPMENT 99: 3 (1987). (10.1242/dev.99.1.3) / DEVELOPMENT (1987)
SYMES, K, GASTRULATION MOVEMENTS PROVIDE AN EARLY MARKER OF MESODERM INDUCTION IN XENOPUS-LAEVIS, DEVELOPMENT 101: 339 (1987). (10.1242/dev.101.2.339) / DEVELOPMENT (1987)
WEEKS, D.L., A MATERNAL MESSENGER-RNA LOCALIZED TO THE VEGETAL HEMISPHERE IN XENOPUS EGGS CODES FOR A GROWTH-FACTOR RELATED TO TGF-BETA, CELL 51: 861 (1987). (10.1016/0092-8674(87)90109-7) / CELL (1987)
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WHITMAN, M, ASSOCIATION OF PHOSPHATIDYLINOSITOL KINASE-ACTIVITY WITH POLYOMA MIDDLE-T COMPETENT FOR TRANSFORMATION, NATURE 315: 239 (1985). (10.1038/315239a0) / NATURE (1985)
WHITMAN, M, TYPE-I PHOSPHATIDYLINOSITOL KINASE MAKES A NOVEL INOSITOL PHOSPHOLIPID, PHOSPHATIDYLINOSITOL-3-PHOSPHATE, NATURE 332: 644 (1988). (10.1038/332644a0) / NATURE (1988)
WHITMAN M unpublished data.
WOODLAND, H.R., THE DEVELOPMENT OF AN ASSAY TO DETECT MESSENGER-RNAS THAT AFFECT EARLY DEVELOPMENT, DEVELOPMENT 101: 925 (1987). (10.1242/dev.101.4.925) / DEVELOPMENT (1987)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 5:35 p.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 4:37 p.m.)
Issued	36 years, 3 months ago (May 19, 1989)
Published	36 years, 3 months ago (May 19, 1989)
Published Print	36 years, 3 months ago (May 19, 1989)

Funders 0

None

BibTeX

@article{Whitman_1989, title={Induction of Mesoderm by a Viral Oncogene in Early Xenopus Embryos}, volume={244}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2658054}, DOI={10.1126/science.2658054}, number={4906}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Whitman, Malcolm and Melton, D. A.}, year={1989}, month=may, pages={803–806} }

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      [
        1989,
        5,
        19
      ]
    ]
  },
  "references-count": 30,
  "journal-issue": {
    "issue": "4906",
    "published-print": {
      "date-parts": [
        [
          1989,
          5,
          19
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.2658054"
  ],
  "URL": "http://dx.doi.org/10.1126/science.2658054",
  "relation": {},
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1989,
        5,
        19
      ]
    ]
  }
}