DOI: 10.1126/science.256.5064.1684. Hydrogen Exchange Measurement of the Free Energy of Structural and Allosteric Change in Hemoglobin [Penn]

Hydrogen Exchange Measurement of the Free Energy of Structural and Allosteric Change in Hemoglobin

10.1126/science.256.5064.1684

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The inability to localize and measure the free energy of protein structure and structure change severely limits protein structure-function investigations. The local unfolding model for protein hydrogen exchange quantitatively relates the free energy of local structural stability with the hydrogen exchange rate of concerted sets of structurally related protons. In tests with a number of modified hemoglobin forms, the loss in structural free energy obtained locally from hydrogen exchange results matches the loss in allosteric free energy measured globally by oxygen-binding and subunit dissociation experiments.

Bibliography

Englander, S. W., Englander, J. J., McKinnie, R. E., Ackers, G. K., Turner, G. J., Westrick, J. A., & Gill, S. J. (1992). Hydrogen Exchange Measurement of the Free Energy of Structural and Allosteric Change in Hemoglobin. Science, 256(5064), 1684â1687.

Authors 7

S. Walter Englander (first)
Joan J. Englander (additional)
Russell E. McKinnie (additional)
Gary K. Ackers (additional)
George J. Turner (additional)
Judy A. Westrick (additional)
Stanley J. Gill (additional)

References 29 Referenced 60

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10.1016/S0065-3233(08)60190-4

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 6:28 p.m.)
Indexed	1 year ago (Aug. 19, 2024, 11:12 a.m.)
Issued	33 years, 2 months ago (June 19, 1992)
Published	33 years, 2 months ago (June 19, 1992)
Published Print	33 years, 2 months ago (June 19, 1992)

Funders 0

None

BibTeX

@article{Englander_1992, title={Hydrogen Exchange Measurement of the Free Energy of Structural and Allosteric Change in Hemoglobin}, volume={256}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.256.5064.1684}, DOI={10.1126/science.256.5064.1684}, number={5064}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Englander, S. Walter and Englander, Joan J. and McKinnie, Russell E. and Ackers, Gary K. and Turner, George J. and Westrick, Judy A. and Gill, Stanley J.}, year={1992}, month=jun, pages={1684–1687} }

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