DOI: 10.1126/science.256.5060.1199. Calmodulin Trapping by Calcium-Calmodulin-Dependent Protein Kinase

Calmodulin Trapping by Calcium-Calmodulin-Dependent Protein Kinase

10.1126/science.256.5060.1199

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Multifunctional calcium-calmodulin-dependent protein kinase (CaM kinase) transduces transient elevations in intracellular calcium into changes in the phosphorylation state and activity of target proteins. By fluorescence emission anisotropy, the affinity of CaM kinase for dansylated calmodulin was measured and found to increase 1000 times after autophosphorylation of the threonine at position 286 of the protein. Autophosphorylation markedly slowed the release of bound calcium-calmodulin; the release time increased from less than a second to several hundred seconds. In essence, calmodulin is trapped by autophosphorylation. The shift in affinity does not occur in a site-directed mutant in which threonine at position 286 has been replaced by a non-phosphorylatable amino acid. These experiments demonstrate the existence of a new state in which calmodulin is bound to CaM kinase even though the concentration of calcium is basal. Calmodulin trapping provides for molecular potentiation of calcium transients and may enable detection of their frequency.

Bibliography

Meyer, T., Hanson, P. I., Stryer, L., & Schulman, H. (1992). Calmodulin Trapping by Calcium-Calmodulin-Dependent Protein Kinase. Science, 256(5060), 1199â1202.

Authors 4

Tobias Meyer (first)
Phyllis I. Hanson (additional)
Lubert Stryer (additional)
Howard Schulman (additional)

References 23 Referenced 501

Cohen P. Calmodulin (1988).
COLBRAN, R. J., Calcium/Calmodulin-Dependent Protein Kinase II, CURRENT TOPICS IN CELLULAR REGULATION 31: 181 (1990). (10.1016/B978-0-12-152831-7.50007-X) / CURRENT TOPICS IN CELLULAR REGULATION / Calcium/Calmodulin-Dependent Protein Kinase II (1990)
ERONDU, N.E., REGIONAL DISTRIBUTION OF TYPE-II CA-2+ CALMODULIN-DEPENDENT PROTEIN-KINASE IN RAT-BRAIN, JOURNAL OF NEUROSCIENCE 5: 3270 (1985). (10.1523/JNEUROSCI.05-12-03270.1985) / JOURNAL OF NEUROSCIENCE (1985)
ESTEP, R.P., CURR TOP CELL REGUL 31: 161 (1990). (10.1016/B978-0-12-152831-7.50006-8) / CURR TOP CELL REGUL (1990)
FONG, Y.L., STUDIES OF THE REGULATORY MECHANISM OF CA-2+-CALMODULIN-DEPENDENT PROTEIN KINASE-II - MUTATION OF THREONINE-286 TO ALANINE AND ASPARTATE, JOURNAL OF BIOLOGICAL CHEMISTRY 264: 16759 (1989). (10.1016/S0021-9258(19)84770-1) / JOURNAL OF BIOLOGICAL CHEMISTRY (1989)
GRAFF, J.M., PHOSPHORYLATION-REGULATED CALMODULIN BINDING TO A PROMINENT CELLULAR SUBSTRATE FOR PROTEIN KINASE-C, JOURNAL OF BIOLOGICAL CHEMISTRY 264: 21818 (1989). (10.1016/S0021-9258(20)88257-X) / JOURNAL OF BIOLOGICAL CHEMISTRY (1989)
HANSON, P.I., NEURONAL CA2+/CALMODULIN-DEPENDENT PROTEIN-KINASES, ANNUAL REVIEW OF BIOCHEMISTRY 61: 559 (1992). (10.1146/annurev.bi.61.070192.003015) / ANNUAL REVIEW OF BIOCHEMISTRY (1992)
HANSON, P.I., EXPRESSION OF A MULTIFUNCTIONAL CA-2+ CALMODULIN-DEPENDENT PROTEIN-KINASE AND MUTATIONAL ANALYSIS OF ITS AUTO-REGULATION, NEURON 3: 59 (1989). (10.1016/0896-6273(89)90115-3) / NEURON (1989)
KENNEDY, M.B., STRUCTURE AND REGULATION OF TYPE-II CALCIUM CALMODULIN-DEPENDENT PROTEIN-KINASE IN CENTRAL-NERVOUS-SYSTEM NEURONS, COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY 55: 101 (1990). (10.1101/SQB.1990.055.01.013) / COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY (1990)
KINCAID, R.L., PREPARATION OF FLUORESCENT, CROSS-LINKING, AND BIOTINYLATED CALMODULIN DERIVATIVES AND THEIR USE IN STUDIES OF CALMODULIN-ACTIVATED PHOSPHODIESTERASE AND PROTEIN PHOSPHATASE, METHODS IN ENZYMOLOGY 159: 605 (1988). (10.1016/0076-6879(88)59058-4) / METHODS IN ENZYMOLOGY (1988)
LAI, Y, CA-2+/CALMODULIN-DEPENDENT PROTEIN KINASE-II - IDENTIFICATION OF AUTOPHOSPHORYLATION SITES RESPONSIBLE FOR GENERATION OF CA-2+/CALMODULIN-INDEPENDENCE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 84: 5710 (1987). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1987)
LOU, L.L., DISTINCT AUTOPHOSPHORYLATION SITES SEQUENTIALLY PRODUCE AUTONOMY AND INHIBITION OF THE MULTIFUNCTIONAL CA2+-CALMODULIN-DEPENDENT PROTEIN-KINASE, JOURNAL OF NEUROSCIENCE 9: 2020 (1989). (10.1523/JNEUROSCI.09-06-02020.1989) / JOURNAL OF NEUROSCIENCE (1989)
MEYER, T, TRANSIENT CALCIUM RELEASE INDUCED BY SUCCESSIVE INCREMENTS OF INOSITOL 1,4,5-TRISPHOSPHATE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 87: 3841 (1990). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1990)
MILLER, S.G., SEQUENCES OF AUTOPHOSPHORYLATION SITES IN NEURONAL TYPE-II CAM KINASE THAT CONTROL CA-2+-INDEPENDENT ACTIVITY, NEURON 1: 593 (1988). (10.1016/0896-6273(88)90109-2) / NEURON (1988)
MINTA, A, FLUORESCENT INDICATORS FOR CYTOSOLIC CALCIUM BASED ON RHODAMINE AND FLUORESCEIN CHROMOPHORES, JOURNAL OF BIOLOGICAL CHEMISTRY 264: 8171 (1989). (10.1016/S0021-9258(18)83165-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1989)
MOLLOY, S.S., AUTOPHOSPHORYLATION OF TYPE-II CA2+ CALMODULIN-DEPENDENT PROTEIN-KINASE IN CULTURES OF POSTNATAL RAT HIPPOCAMPAL SLICES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 4756 (1991). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1991)
ROSENBERG, G.B., RECONSTITUTION OF CALMODULIN-SENSITIVE ADENYLATE-CYCLASE FROM BOVINE BRAIN WITH PHOSPHOLIPIDS, CALMODULIN, AND BETA-ADRENERGIC RECEPTORS, METHODS IN ENZYMOLOGY 139: 776 (1987). (10.1016/0076-6879(87)39126-8) / METHODS IN ENZYMOLOGY (1987)
SCHULMAN, H, CURR OPIN NEUROBIOL 1: 43 (1991). (10.1016/0959-4388(91)90009-V) / CURR OPIN NEUROBIOL (1991)
SCHULMAN, H, PHOSPHORYLATION OF MICROTUBULE-ASSOCIATED PROTEINS BY A CA-2+/CALMODULIN-DEPENDENT PROTEIN-KINASE, JOURNAL OF CELL BIOLOGY 99: 11 (1984). (10.1083/jcb.99.1.11) / JOURNAL OF CELL BIOLOGY (1984)
SCHWORER, C.M., CA-2+/CALMODULIN-DEPENDENT PROTEIN KINASE-II, JOURNAL OF BIOLOGICAL CHEMISTRY 263: 13486 (1988). (10.1016/S0021-9258(18)68264-X) / JOURNAL OF BIOLOGICAL CHEMISTRY (1988)
SKENE, J. H. P., NEUROSCIENCE RESEARCH 13: S112 (1990). / NEUROSCIENCE RESEARCH (1990)
THIEL, G, CA-2+/CALMODULIN-DEPENDENT PROTEIN KINASE-II - IDENTIFICATION OF THREONINE-286 AS THE AUTOPHOSPHORYLATION SITE IN THE ALPHA-SUBUNIT ASSOCIATED WITH THE GENERATION OF CA-2+-INDEPENDENT ACTIVITY, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 6337 (1988). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1988)
YAMAUCHI, T, EXPRESSION AND CHARACTERIZATION OF CALMODULIN-DEPENDENT PROTEIN KINASE-II FROM CLONED CDNAS IN CHINESE-HAMSTER OVARY CELLS, JOURNAL OF BIOLOGICAL CHEMISTRY 264: 19108 (1989). (10.1016/S0021-9258(19)47274-8) / JOURNAL OF BIOLOGICAL CHEMISTRY (1989)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 4:05 p.m.)
Indexed	2 months ago (June 16, 2025, 12:04 p.m.)
Issued	33 years, 3 months ago (May 22, 1992)
Published	33 years, 3 months ago (May 22, 1992)
Published Print	33 years, 3 months ago (May 22, 1992)

Funders 0

None

BibTeX

@article{Meyer_1992, title={Calmodulin Trapping by Calcium-Calmodulin-Dependent Protein Kinase}, volume={256}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.256.5060.1199}, DOI={10.1126/science.256.5060.1199}, number={5060}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Meyer, Tobias and Hanson, Phyllis I. and Stryer, Lubert and Schulman, Howard}, year={1992}, month=may, pages={1199–1202} }

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