DOI: 10.1126/science.256.5058.825. Implication of GAP in Ras-Dependent Transactivation of a Polyoma Enhancer Sequence

Implication of GAP in Ras-Dependent Transactivation of a Polyoma Enhancer Sequence

10.1126/science.256.5058.825

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Controversy exists as to whether the interaction of a guanosine triphosphatase activating protein (GAP) with Ras proteins functions both to initiate and to terminate Ras-dependent signaling events or only to terminate them. GAP-C, a carboxyl-terminal fragment of GAP that is sufficient to stimulate GTPase activity, inhibited the stimulation of transcription produced by some oncoproteins (v-Src, polyoma middle T, wild-type Ras, and oncogenic Ras) but not that produced by v-Mos. Wild-type GAP did not affect transcription induced by oncogenic Ras but reversed the inhibitory effect of GAP-C on transcription induced by oncogenic Ras. These results indicate that GAP is a negative regulator of wild-type Ras and elicits a downstream signal by interacting with Ras-GTP (guanosine triphosphate).

Bibliography

Schweighoffer, F., Barlat, I., Chevallier-Multon, M.-C., & Tocque, B. (1992). Implication of GAP in Ras-Dependent Transactivation of a Polyoma Enhancer Sequence. Science, 256(5058), 825â827.

Authors 4

Fabien Schweighoffer (first)
Isabelle Barlat (additional)
Marie-Christine Chevallier-Multon (additional)
Bruno Tocque (additional)

References 32 Referenced 22

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CALES, C, THE CYTOPLASMIC PROTEIN GAP IS IMPLICATED AS THE TARGET FOR REGULATION BY THE RAS GENE-PRODUCT, NATURE 332: 548 (1988). (10.1038/332548a0) / NATURE (1988)
COURTNEIDGE, S.A., EMBO JOURNAL 4: 1471 (1985). (10.1002/j.1460-2075.1985.tb03805.x) / EMBO JOURNAL (1985)
DECLUE, J.E., SUPPRESSION OF SRC TRANSFORMATION BY OVEREXPRESSION OF FULL-LENGTH GTPASE-ACTIVATING PROTEIN (GAP) OR OF THE GAP-C TERMINUS, MOLECULAR AND CELLULAR BIOLOGY 11: 2819 (1991). / MOLECULAR AND CELLULAR BIOLOGY (1991)
DECLUE, J.E., A RAS EFFECTOR DOMAIN MUTANT WHICH IS TEMPERATURE SENSITIVE FOR CELLULAR-TRANSFORMATION - INTERACTIONS WITH GTPASE-ACTIVATING PROTEIN AND NF-1, MOLECULAR AND CELLULAR BIOLOGY 11: 3132 (1991). / MOLECULAR AND CELLULAR BIOLOGY (1991)
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FEIG, L.A., INHIBITION OF NIH-3T3 CELL-PROLIFERATION BY A MUTANT RAS PROTEIN WITH PREFERENTIAL AFFINITY FOR GDP, MOLECULAR AND CELLULAR BIOLOGY 8: 3235 (1988). / MOLECULAR AND CELLULAR BIOLOGY (1988)
10.1126/science.2164710
GIBBS, J.B., MODULATION OF GUANINE-NUCLEOTIDES BOUND TO RAS IN NIH3T3 CELLS BY ONCOGENES, GROWTH-FACTORS, AND THE GTPASE ACTIVATING PROTEIN (GAP), JOURNAL OF BIOLOGICAL CHEMISTRY 265: 20437 (1990). (10.1016/S0021-9258(17)30523-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1990)
10.1073/pnas.85.14.5026
GIBBS, J.B., XENOPUS OOCYTE GERMINAL-VESICLE BREAKDOWN INDUCED BY [VAL12]RAS IS INHIBITED BY A CYTOSOL-LOCALIZED RAS MUTANT, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86: 6630 (1989). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1989)
GORMAN, C.M., RECOMBINANT GENOMES WHICH EXPRESS CHLORAMPHENICOL ACETYLTRANSFERASE IN MAMMALIAN-CELLS, MOLECULAR AND CELLULAR BIOLOGY 2: 1044 (1982). / MOLECULAR AND CELLULAR BIOLOGY (1982)
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IMLER, J.L., A HARVEY-RAS RESPONSIVE TRANSCRIPTION ELEMENT IS ALSO RESPONSIVE TO A TUMOR-PROMOTER AND TO SERUM, NATURE 332: 275 (1988). (10.1038/332275a0) / NATURE (1988)
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LLOYD, A, NATURE 352: 635 (1991). (10.1038/352635a0) / NATURE (1991)
MARSHALL, C.J., HOW DOES P21 RAS TRANSFORM CELLS, TRENDS IN GENETICS 7: 91 (1991). (10.1016/0168-9525(91)90278-X) / TRENDS IN GENETICS (1991)
MARSHALL, M.S., A C-TERMINAL DOMAIN OF GAP IS SUFFICIENT TO STIMULATE RAS P21 GTPASE ACTIVITY, EMBO JOURNAL 8: 1105 (1989). (10.1002/j.1460-2075.1989.tb03480.x) / EMBO JOURNAL (1989)
10.1016/0092-8674(90)90150-D
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MCCORMICK, F, THE WORLD ACCORDING TO GAP, ONCOGENE 5: 1281 (1990). / ONCOGENE (1990)
NORI, M, INHIBITION OF V-SRC-INDUCED TRANSFORMATION BY A GTPASE-ACTIVATING PROTEIN, MOLECULAR AND CELLULAR BIOLOGY 11: 2812 (1991). / MOLECULAR AND CELLULAR BIOLOGY (1991)
REY, I, THE COOH-DOMAIN OF THE PRODUCT OF THE SACCHAROMYCES-CEREVISIAE SCD25 GENE ELICITS ACTIVATION OF P21-RAS PROTEINS IN MAMMALIAN-CELLS, ONCOGENE 6: 347 (1991). / ONCOGENE (1991)
SASSONECORSI, P, RAS-INDUCED NEURONAL DIFFERENTIATION OF PC12 CELLS - POSSIBLE INVOLVEMENT OF FOS AND JUN, MOLECULAR AND CELLULAR BIOLOGY 9: 3174 (1989). / MOLECULAR AND CELLULAR BIOLOGY (1989)
SCHWEIGHOFFER F unpublished data.
STACEY, D.W., CRITICAL ROLE OF CELLULAR RAS PROTEINS IN PROLIFERATIVE SIGNAL TRANSDUCTION, COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY 53: 871 (1988). (10.1101/SQB.1988.053.01.100) / COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY (1988)
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10.1016/0092-8674(90)90024-9
YATANI, A, RAS P21 AND GAP INHIBIT COUPLING OF MUSCARINIC RECEPTORS TO ATRIAL K+ CHANNELS, CELL 61: 769 (1990). (10.1016/0092-8674(90)90187-J) / CELL (1990)
10.1038/346754a0

Dates

Type	When
Created	3 years, 10 months ago (Oct. 10, 2021, 9:58 a.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 6:31 p.m.)
Indexed	1 year, 7 months ago (Jan. 15, 2024, 1:02 a.m.)
Issued	33 years, 3 months ago (May 8, 1992)
Published	33 years, 3 months ago (May 8, 1992)
Published Print	33 years, 3 months ago (May 8, 1992)

Funders 0

None

BibTeX

@article{Schweighoffer_1992, title={Implication of GAP in Ras-Dependent Transactivation of a Polyoma Enhancer Sequence}, volume={256}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.256.5058.825}, DOI={10.1126/science.256.5058.825}, number={5058}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Schweighoffer, Fabien and Barlat, Isabelle and Chevallier-Multon, Marie-Christine and Tocque, Bruno}, year={1992}, month=may, pages={825–827} }

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