DOI: 10.1126/science.256.5055.382. Cell Cycle—Regulated Binding of c-Abl Tyrosine Kinase to DNA

Cell Cycle—Regulated Binding of c-Abl Tyrosine Kinase to DNA

10.1126/science.256.5055.382

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The proto-oncogene c- abl encodes a protein tyrosine kinase that is localized in the cytoplasm and the nucleus. The large carboxyl-terminal segment of c-Abl was found to contain a DNA-binding domain that was necessary for the association of c-Abl with chromatin. The DNA-binding activity of c-Abl was lost during mitosis when the carboxyl-terminal segment became phosphorylated. In vitro phosphorylation of the DNA-binding domain by cdc2 kinase abolished DNA binding. Homozygous mutant mice expressing a c-Abl tyrosine kinase without the DNA-binding domain have been reported to die of multiple defects at birth. Thus, binding of the c-Abl tyrosine kinase to DNA may be essential to its biological function.

Bibliography

Kipreos, E. T., & Wang, J. Y. J. (1992). Cell CycleâRegulated Binding of c-Abl Tyrosine Kinase to DNA. Science, 256(5055), 382â385.

Authors 2

Edward T. Kipreos (first)
Jean Y. J. Wang (additional)

References 12 Referenced 149

BOWEN, B, DETECTION OF DNA-BINDING PROTEINS BY PROTEIN BLOTTING, NUCLEIC ACIDS RESEARCH 8: 1 (1980). (10.1093/nar/8.1.1) / NUCLEIC ACIDS RESEARCH (1980)
GUAN, K.L., EUKARYOTIC PROTEINS EXPRESSED IN ESCHERICHIA-COLI - AN IMPROVED THROMBIN CLEAVAGE AND PURIFICATION PROCEDURE OF FUSION PROTEINS WITH GLUTATHIONE-S-TRANSFERASE, ANALYTICAL BIOCHEMISTRY 192: 262 (1991). (10.1016/0003-2697(91)90534-Z) / ANALYTICAL BIOCHEMISTRY (1991)
HANCOCK, R, Isolation of Interphase Chromatin Structures from Cultured Cells, METHODS IN CELL BIOLOGY 15: 127 (1977). (10.1016/S0091-679X(08)60213-7) / METHODS IN CELL BIOLOGY / Isolation of Interphase Chromatin Structures from Cultured Cells (1977)
10.1126/science.2183353
10.1126/science.1708916
MCWHIRTER, J.R., ACTIVATION OF TYROSINE KINASE AND MICROFILAMENT-BINDING FUNCTIONS OF C-ABL BY BCR SEQUENCES IN BCR/ABL FUSION PROTEINS, MOLECULAR AND CELLULAR BIOLOGY 11: 1553 (1991). / MOLECULAR AND CELLULAR BIOLOGY (1991)
SCHWARTZBERG, P.L., MICE HOMOZYGOUS FOR THE ABLM1 MUTATION SHOW POOR VIABILITY AND DEPLETION OF SELECTED B-CELL AND T-CELL POPULATIONS, CELL 65: 1165 (1991). (10.1016/0092-8674(91)90012-N) / CELL (1991)
TYBULEWICZ, VLJ, NEONATAL LETHALITY AND LYMPHOPENIA IN MICE WITH A HOMOZYGOUS DISRUPTION OF THE C-ABL PROTOONCOGENE, CELL 65: 1153 (1991). (10.1016/0092-8674(91)90011-M) / CELL (1991)
VANETTEN, R.A., THE MOUSE TYPE-IV C-ABL GENE-PRODUCT IS A NUCLEAR-PROTEIN, AND ACTIVATION OF TRANSFORMING ABILITY IS ASSOCIATED WITH CYTOPLASMIC LOCALIZATION, CELL 58: 669 (1989). (10.1016/0092-8674(89)90102-5) / CELL (1989)
WANG, JYJ, THE MOUSE C-ABL LOCUS - MOLECULAR-CLONING AND CHARACTERIZATION, CELL 36: 349 (1984). (10.1016/0092-8674(84)90228-9) / CELL (1984)
WANG, JYJ, NEGATIVE REGULATION OF C-ABL TYROSINE KINASE BY ITS VARIABLE N-TERMINAL AMINO-ACIDS, ONCOGENE RESEARCH 3: 293 (1988). / ONCOGENE RESEARCH (1988)
WANG, N.P., DNA-BINDING ACTIVITY OF RETINOBLASTOMA PROTEIN IS INTRINSIC TO ITS CARBOXYL-TERMINAL REGION, CELL GROWTH & DIFFERENTIATION 1: 233 (1990). / CELL GROWTH & DIFFERENTIATION (1990)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 6:32 p.m.)
Indexed	3 months, 2 weeks ago (May 5, 2025, 5:02 a.m.)
Issued	33 years, 4 months ago (April 17, 1992)
Published	33 years, 4 months ago (April 17, 1992)
Published Print	33 years, 4 months ago (April 17, 1992)

Funders 0

None

BibTeX

@article{Kipreos_1992, title={Cell Cycle—Regulated Binding of c-Abl Tyrosine Kinase to DNA}, volume={256}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.256.5055.382}, DOI={10.1126/science.256.5055.382}, number={5055}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Kipreos, Edward T. and Wang, Jean Y. J.}, year={1992}, month=apr, pages={382–385} }

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