DOI: 10.1126/science.2556795. Inhibition of Antigen-Induced Lymphocyte Proliferation by Tat Protein from HIV-1

Inhibition of Antigen-Induced Lymphocyte Proliferation by Tat Protein from HIV-1

10.1126/science.2556795

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The purified human immunodeficiency virus type-1 (HIV-1) Tat protein inhibited lymphocyte proliferation induced by tetanus toxoid or Candida antigens by 66 to 97% at nanomolar concentrations of Tat. In contrast, Tat did not cause a significant reduction of lymphocyte proliferation in response to mitogens such as phytohemagglutinin or pokeweed mitogen. Inhibition was blocked by oxidation of the cysteine-rich region of Tat or by incubation with an antibody to Tat before the assay. A synthetic Tat peptide (residues 1 to 58) also inhibited antigen-stimulated proliferation. Experiments with H9 and U937 cell lines showed that Tat can easily enter both lymphocytes and monocytes. The specific inhibition of antigen-induced lymphocyte proliferation by Tat mimics the effect seen with lymphocytes from HIV-infected individuals and suggests that Tat might directly contribute to the immunosuppression associated with HIV infection.

Bibliography

Viscidi, R. P., Mayur, K., Lederman, H. M., & Frankel, A. D. (1989). Inhibition of Antigen-Induced Lymphocyte Proliferation by Tat Protein from HIV-1. Science, 246(4937), 1606â1608.

Authors 4

Raphael P. Viscidi (first)
Kumudini Mayur (additional)
Howard M. Lederman (additional)
Alan D. Frankel (additional)

References 19 Referenced 276

ALLEN, P.M., ANTIGEN PROCESSING AT THE MOLECULAR-LEVEL, IMMUNOLOGY TODAY 8: 270 (1987). (10.1016/0167-5699(87)90187-3) / IMMUNOLOGY TODAY (1987)
DAYTON, A.I., THE TRANSACTIVATOR GENE OF THE HUMAN T-CELL LYMPHOTROPIC VIRUS TYPE-III IS REQUIRED FOR REPLICATION, CELL 44: 941 (1986). (10.1016/0092-8674(86)90017-6) / CELL (1986)
10.1126/science.3277274
10.1126/science.3422113
FISHER, A.G., THE TRANSACTIVATOR GENE OF HTLV-III IS ESSENTIAL FOR VIRUS-REPLICATION, NATURE 320: 367 (1986). (10.1038/320367a0) / NATURE (1986)
FRANKEL, A.D., CELLULAR UPTAKE OF THE TAT PROTEIN FROM HUMAN IMMUNODEFICIENCY VIRUS, CELL 55: 1189 (1988). (10.1016/0092-8674(88)90263-2) / CELL (1988)
FRANKEL, A.D., DIMERIZATION OF THE TAT PROTEIN FROM HUMAN IMMUNODEFICIENCY VIRUS - A CYSTEINE-RICH PEPTIDE MIMICS THE NORMAL METAL-LINKED DIMER INTERFACE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 6297 (1988). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1988)
FRANKEL, A.D., ACTIVITY OF SYNTHETIC PEPTIDES FROM THE TAT PROTEIN OF HUMAN IMMUNODEFICIENCY VIRUS TYPE-1, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86: 7397 (1989). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1989)
10.1126/science.2832944
GARCIA, J.A., FUNCTIONAL DOMAINS REQUIRED FOR TAT-INDUCED TRANSCRIPTIONAL ACTIVATION OF THE HIV-1 LONG TERMINAL REPEAT, EMBO JOURNAL 7: 3143 (1988). (10.1002/j.1460-2075.1988.tb03181.x) / EMBO JOURNAL (1988)
GORMAN, C.M., RECOMBINANT GENOMES WHICH EXPRESS CHLORAMPHENICOL ACETYLTRANSFERASE IN MAMMALIAN-CELLS, MOLECULAR AND CELLULAR BIOLOGY 2: 1044 (1982). / MOLECULAR AND CELLULAR BIOLOGY (1982)
GURLEY, R.J., CD4+ LYMPHOCYTE FUNCTION WITH EARLY HUMAN IMMUNODEFICIENCY VIRUS-INFECTION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86: 1993 (1989). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1989)
LANE, H.C., QUALITATIVE-ANALYSIS OF IMMUNE FUNCTION IN PATIENTS WITH THE ACQUIRED IMMUNODEFICIENCY SYNDROME - EVIDENCE FOR A SELECTIVE DEFECT IN SOLUBLE-ANTIGEN RECOGNITION, NEW ENGLAND JOURNAL OF MEDICINE 313: 79 (1985). (10.1056/NEJM198507113130204) / NEW ENGLAND JOURNAL OF MEDICINE (1985)
RUBEN, S, STRUCTURAL AND FUNCTIONAL-CHARACTERIZATION OF HUMAN IMMUNODEFICIENCY VIRUS TAT PROTEIN, JOURNAL OF VIROLOGY 63: 1 (1989). (10.1128/jvi.63.1.1-8.1989) / JOURNAL OF VIROLOGY (1989)
SADAIE, M.R., MISSENSE MUTATIONS IN AN INFECTIOUS HUMAN IMMUNODEFICIENCY VIRAL GENOME - FUNCTIONAL MAPPING OF TAT AND IDENTIFICATION OF THE REV SPLICE ACCEPTOR, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 9224 (1988). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1988)
SHEARER, G.M., A MODEL FOR THE SELECTIVE LOSS OF MAJOR HISTOCOMPATIBILITY COMPLEX SELF-RESTRICTED T-CELL IMMUNE-RESPONSES DURING THE DEVELOPMENT OF ACQUIRED-IMMUNE-DEFICIENCY-SYNDROME (AIDS), JOURNAL OF IMMUNOLOGY 137: 2514 (1986). (10.4049/jimmunol.137.8.2514) / JOURNAL OF IMMUNOLOGY (1986)
10.1126/science.2981427
VISCIDI R.P. unpublished data.
WEISS, A, THE ROLE OF THE T3-ANTIGEN RECEPTOR COMPLEX IN T-CELL ACTIVATION, ANNUAL REVIEW OF IMMUNOLOGY 4: 593 (1986). (10.1146/annurev.iy.04.040186.003113) / ANNUAL REVIEW OF IMMUNOLOGY (1986)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 4:06 p.m.)
Indexed	1 month, 1 week ago (July 12, 2025, 6:57 p.m.)
Issued	35 years, 8 months ago (Dec. 22, 1989)
Published	35 years, 8 months ago (Dec. 22, 1989)
Published Print	35 years, 8 months ago (Dec. 22, 1989)

Funders 0

None

BibTeX

@article{Viscidi_1989, title={Inhibition of Antigen-Induced Lymphocyte Proliferation by Tat Protein from HIV-1}, volume={246}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2556795}, DOI={10.1126/science.2556795}, number={4937}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Viscidi, Raphael P. and Mayur, Kumudini and Lederman, Howard M. and Frankel, Alan D.}, year={1989}, month=dec, pages={1606–1608} }

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