DOI: 10.1126/science.2530632. Cognate DNA Binding Specificity Retained After Leucine Zipper Exchange Between GCN4 and C/EBP

Cognate DNA Binding Specificity Retained After Leucine Zipper Exchange Between GCN4 and C/EBP

10.1126/science.2530632

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Both C/EBP and GCN4 are sequence-specific DNA binding proteins that control gene expression. Recent evidence implicates C/EBP as a transcriptional regulator of genes involved in lipid and carbohydrate metabolism. The C/EBP protein binds avidly to the dyad symmetric sequence 5′-ATTGCGCAAT-3′; GCN4 regulates the transcription of genes that control amino acid biosynthesis in yeast, and binds avidly to the dyad symmetric sequence 5′-ATGA(G/C)TCAT-3′. Both C/EBP and GCN4 bind DNA via the same structural motif. This motif has been predicted to be bipartite, consisting of a dimerization interface termed the "leucine zipper" and a DNA contact surface termed the "basic region." Specificity of DNA binding has been predicted to be imparted by the basic region. As a test of this hypothesis, recombinant proteins were created wherein the basic regions and leucine zippers of GCN4 and C/EBP were reciprocally exchanged. In both of the recombinant polypeptides, DNA binding specificity is shown to track with the basic region.

Bibliography

Agre, P., Johnson, P. F., & McKnight, S. L. (1989). Cognate DNA Binding Specificity Retained After Leucine Zipper Exchange Between GCN4 and C/EBP. Science, 246(4932), 922â926.

Authors 3

Peter Agre (first)
Peter F. Johnson (additional)
Steven L. McKnight (additional)

References 33 Referenced 160

AGRE P unpublished data.
10.1073/pnas.83.22.8516
CRICK, FHC, IS ALPHA-KERATIN A COILED COIL, NATURE 170: 882 (1952). / NATURE (1952)
CURRAN, T, CANDIDATE PRODUCT OF THE FBJ MURINE OSTEO-SARCOMA VIRUS ONCOGENE - CHARACTERIZATION OF A 55,000-DALTON PHOSPHOPROTEIN, JOURNAL OF VIROLOGY 42: 114 (1982). (10.1128/jvi.42.1.114-122.1982) / JOURNAL OF VIROLOGY (1982)
CURRAN, T, FBJ MURINE OSTEO-SARCOMA VIRUS - IDENTIFICATION AND MOLECULAR-CLONING OF BIOLOGICALLY-ACTIVE PROVIRAL DNA, JOURNAL OF VIROLOGY 44: 674 (1982). (10.1128/jvi.44.2.674-682.1982) / JOURNAL OF VIROLOGY (1982)
10.1126/science.2964084
FU, Y.H., CYS-3, THE POSITIVE-ACTING SULFUR REGULATORY GENE OF NEUROSPORA-CRASSA, ENCODES A PROTEIN WITH A PUTATIVE LEUCINE ZIPPER DNA-BINDING ELEMENT, MOLECULAR AND CELLULAR BIOLOGY 9: 1120 (1989). / MOLECULAR AND CELLULAR BIOLOGY (1989)
10.1126/science.2494702
GRAVES, B.J., HOMOLOGOUS RECOGNITION OF A PROMOTER DOMAIN COMMON TO THE MSV LTR AND THE HSV TK GENE, CELL 44: 565 (1986). (10.1016/0092-8674(86)90266-7) / CELL (1986)
10.1126/science.3532321
HINNEBUSCH, A.G., EVIDENCE FOR TRANSLATIONAL REGULATION OF THE ACTIVATOR OF GENERAL AMINO-ACID CONTROL IN YEAST, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 81: 6442 (1984). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1984)
10.1016/0092-8674(85)90022-4
HOPE, I.A., FUNCTIONAL DISSECTION OF A EUKARYOTIC TRANSCRIPTIONAL ACTIVATOR PROTEIN, GCN4 OF YEAST, CELL 46: 885 (1986). (10.1016/0092-8674(86)90070-X) / CELL (1986)
10.1101/gad.1.2.133
KOUZARIDES, T, THE ROLE OF THE LEUCINE ZIPPER IN THE FOS JUN INTERACTION, NATURE 336: 646 (1988). (10.1038/336646a0) / NATURE (1988)
LANDSCHULZ, W.H., ISOLATION OF A RECOMBINANT COPY OF THE GENE ENCODING C/EBP, GENES & DEVELOPMENT 2: 786 (1988). (10.1101/gad.2.7.786) / GENES & DEVELOPMENT (1988)
10.1126/science.3289117
10.1126/science.2494700
10.1016/0092-8674(87)90612-X
10.1038/325368a0
10.1073/pnas.84.9.2848
Maniatis T. Molecular Cloning: A Laboratory Manual (1982).
10.1126/science.6283634
NEUBERG, M, 2 FUNCTIONALLY DIFFERENT REGIONS IN FOS ARE REQUIRED FOR THE SEQUENCE-SPECIFIC DNA INTERACTION OF THE FOS JUN PROTEIN COMPLEX, NATURE 338: 589 (1989). (10.1038/338589a0) / NATURE (1989)
10.1126/science.3130660
SASSONECORSI, P, DIRECT INTERACTION BETWEEN FOS AND JUN NUCLEAR ONCOPROTEINS - ROLE OF THE LEUCINE ZIPPER DOMAIN, NATURE 336: 692 (1988). (10.1038/336692a0) / NATURE (1988)
SHUMAN J unpublished data.
STUDIER, F.W., USE OF BACTERIOPHAGE-T7 RNA-POLYMERASE TO DIRECT SELECTIVE HIGH-LEVEL EXPRESSION OF CLONED GENES, JOURNAL OF MOLECULAR BIOLOGY 189: 113 (1986). (10.1016/0022-2836(86)90385-2) / JOURNAL OF MOLECULAR BIOLOGY (1986)
THIREOS, G, 5' UNTRANSLATED SEQUENCES ARE REQUIRED FOR THE TRANSLATIONAL CONTROL OF A YEAST REGULATORY GENE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 81: 5096 (1984). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1984)
10.1126/science.2494701
10.1126/science.2683088
10.1073/pnas.84.10.3316
ZOLLER, M.J., Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors, METHODS IN ENZYMOLOGY 100: 468 (1983). (10.1016/0076-6879(83)00074-9) / METHODS IN ENZYMOLOGY / Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors (1983)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 9:49 a.m.)
Indexed	3 months, 2 weeks ago (May 19, 2025, 4:23 p.m.)
Issued	35 years, 9 months ago (Nov. 17, 1989)
Published	35 years, 9 months ago (Nov. 17, 1989)
Published Print	35 years, 9 months ago (Nov. 17, 1989)

Funders 0

None

BibTeX

@article{Agre_1989, title={Cognate DNA Binding Specificity Retained After Leucine Zipper Exchange Between GCN4 and C/EBP}, volume={246}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2530632}, DOI={10.1126/science.2530632}, number={4932}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Agre, Peter and Johnson, Peter F. and McKnight, Steven L.}, year={1989}, month=nov, pages={922–926} }

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