DOI: 10.1126/science.2499043. G <sub>olf</sub> : an Olfactory Neuron Specific-G Protein Involved in Odorant Signal Transduction

G olf : an Olfactory Neuron Specific-G Protein Involved in Odorant Signal Transduction

10.1126/science.2499043

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Biochemical and electrophysiological studies suggest that odorants induce responses in olfactory sensory neurons via an adenylate cyclase cascade mediated by a G protein. An olfactory-specific guanosine triphosphate (GTP)-binding protein α subunit has now been characterized and evidence is presented suggesting that this G protein, termed G olf , mediates olfaction. Messenger RNA that encodes G olfα is expressed in olfactory neuroephithelium but not in six other tissues tested. Moreover, within the olfactory epithelium, G olfα appears to be expressed only by the sensory neurons. Specific antisera were used to localize G olfα protein to the sensory apparatus of the receptor neurons. G olfα shares extensive amino acid identity (88 percent) with the stimulatory G protein, G sα . The expression of G olfα in S49 cyc - kin - cells, a line deficient in endogenous stimulatory G proteins, demonstrates its capacity to stimulate adenylate cyclase in a heterologous system.

Authors 2

David T. Jones (first)
Randall R. Reed (additional)

References 40 Referenced 682

ANHOLT, RRH, TRANSDUCTION PROTEINS OF OLFACTORY RECEPTOR-CELLS - IDENTIFICATION OF GUANINE-NUCLEOTIDE BINDING-PROTEINS AND PROTEIN-KINASE-C, BIOCHEMISTRY 26: 788 (1987). (10.1021/bi00377a020) / BIOCHEMISTRY (1987)
Baker, T. A., Proceedings of the National Academy of Sciences of the United States of America 81: 6779 (1984). / Proceedings of the National Academy of Sciences of the United States of America (1984)
Beets, M. G. J., Fragrance Chemistry: The Science of Sense and Smell: 93 (1982). / Fragrance Chemistry: The Science of Sense and Smell (1982)
BRAY, P, HUMAN CDNA CLONES FOR 4 SPECIES OF G-ALPHA-S SIGNAL TRANSDUCTION PROTEIN, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 8893 (1986). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1986)
CERIONE, R.A., SPECIFICITY OF THE FUNCTIONAL INTERACTIONS OF THE BETA-ADRENERGIC-RECEPTOR AND RHODOPSIN WITH GUANINE-NUCLEOTIDE REGULATORY PROTEINS RECONSTITUTED IN PHOSPHOLIPID-VESICLES, JOURNAL OF BIOLOGICAL CHEMISTRY 260: 1493 (1985). (10.1016/S0021-9258(18)89619-3) / JOURNAL OF BIOLOGICAL CHEMISTRY (1985)
CULLEN, E.I., BIOSYNTHESIS OF AMIDATED JOINING PEPTIDE FROM PRO-ADRENOCORTICOTROPIN-ENDORPHIN, MOLECULAR ENDOCRINOLOGY 1: 583 (1987). (10.1210/mend-1-9-583) / MOLECULAR ENDOCRINOLOGY (1987)
10.1016/0003-2697(83)90418-9
FONG, HKW, IDENTIFICATION OF A GTP-BINDING PROTEIN ALPHA-SUBUNIT THAT LACKS AN APPARENT ADP-RIBOSYLATION SITE FOR PERTUSSIS TOXIN, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 3066 (1988). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1988)
10.1146/annurev.bi.56.070187.003151
GRAZIADEI, GAM, IMMUNOCYTOCHEMISTRY OF OLFACTORY MARKER PROTEIN, JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 25: 1311 (1977). (10.1177/25.12.336785) / JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY (1977)
GRAZIADEI, GAM, NEUROGENESIS AND NEURON REGENERATION IN THE OLFACTORY SYSTEM OF MAMMALS .2. DEGENERATION AND RECONSTITUTION OF THE OLFACTORY SENSORY NEURONS AFTER AXOTOMY, JOURNAL OF NEUROCYTOLOGY 8: 197 (1979). (10.1007/BF01175561) / JOURNAL OF NEUROCYTOLOGY (1979)
GREEN, N, IMMUNOGENIC STRUCTURE OF THE INFLUENZA-VIRUS HEMAGGLUTININ, CELL 28: 477 (1982). (10.1016/0092-8674(82)90202-1) / CELL (1982)
HALLIDAY, K.R., REGIONAL HOMOLOGY IN GTP-BINDING PROTO-ONCOGENE PRODUCTS AND ELONGATION-FACTORS, JOURNAL OF CYCLIC NUCLEOTIDE AND PROTEIN PHOSPHORYLATION RESEARCH 9: 435 (1984). / JOURNAL OF CYCLIC NUCLEOTIDE AND PROTEIN PHOSPHORYLATION RESEARCH (1984)
HARRIS, B.A., MOLECULAR-CLONING OF COMPLEMENTARY-DNA FOR THE ALPHA-SUBUNIT OF THE G-PROTEIN THAT STIMULATES ADENYLATE-CYCLASE, SCIENCE 229: 1274 (1985). (10.1126/science.3839937) / SCIENCE (1985)
Jones, D. T., Cold Spring Harbor Symposia on Quantitative Biology 53: 349 (1988). (10.1101/SQB.1988.053.01.042) / Cold Spring Harbor Symposia on Quantitative Biology (1988)
10.1016/S0021-9258(18)47929-X
KIRSCHMEIER, P.T., DNA 7: 219 (1988). (10.1089/dna.1988.7.219) / DNA (1988)
10.1093/nar/12.2.857
KOZASA, T, ISOLATION AND CHARACTERIZATION OF THE HUMAN GS-ALPHA GENE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 2081 (1988). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1988)
LANCET, D, VERTEBRATE OLFACTORY RECEPTION, ANNUAL REVIEW OF NEUROSCIENCE 9: 329 (1986). (10.1146/annurev.ne.09.030186.001553) / ANNUAL REVIEW OF NEUROSCIENCE (1986)
10.1126/science.3529395
MACHLIN P unpublished data.
MASTERS, S.B., NATURE 241: 448 (1988). / NATURE (1988)
MASTERS, S.B., FAMILY OF G-PROTEIN ALPHA-CHAINS - AMPHIPATHIC ANALYSIS AND PREDICTED STRUCTURE OF FUNCTIONAL DOMAINS, PROTEIN ENGINEERING 1: 47 (1986). / PROTEIN ENGINEERING (1986)
MATSUOKA, M, SEQUENCE-ANALYSIS OF CDNA AND GENOMIC DNA FOR A PUTATIVE PERTUSSIS TOXIN-INSENSITIVE GUANINE NUCLEOTIDE-BINDING REGULATORY PROTEIN ALPHA-SUBUNIT, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 5384 (1988). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1988)
MUMBY, S.M., ANTISERA OF DESIGNED SPECIFICITY FOR SUBUNITS OF GUANINE NUCLEOTIDE-BINDING REGULATORY PROTEINS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 265 (1986). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1986)
10.1038/325442a0
10.1038/316255a0
PACE, U, OLFACTORY GTP-BINDING PROTEIN - SIGNAL-TRANSDUCING POLYPEPTIDE OF VERTEBRATE CHEMOSENSORY NEURONS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 4947 (1986). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1986)
PEVSNER, J, ISOLATION AND CHARACTERIZATION OF AN OLFACTORY RECEPTOR PROTEIN FOR ODORANT PYRAZINES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 82: 3050 (1985). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1985)
Rhein, L. D., Proceedings of the National Academy of Sciences of the United States of America 77: 4412 (1980). / Proceedings of the National Academy of Sciences of the United States of America (1980)
ROBISHAW, J.D., MOLECULAR-BASIS FOR 2 FORMS OF THE G-PROTEIN THAT STIMULATES ADENYLATE-CYCLASE, JOURNAL OF BIOLOGICAL CHEMISTRY 261: 9587 (1986). (10.1016/S0021-9258(18)67553-2) / JOURNAL OF BIOLOGICAL CHEMISTRY (1986)
ROSS, E.M., RELATIONSHIP BETWEEN BETA-ADRENERGIC-RECEPTOR AND ADENYLATE-CYCLASE - STUDIES OF LIGAND-BINDING AND ENZYME-ACTIVITY IN PURIFIED MEMBRANES OF S49 LYMPHOMA-CELLS, JOURNAL OF BIOLOGICAL CHEMISTRY 252: 5761 (1977). (10.1016/S0021-9258(17)40089-5) / JOURNAL OF BIOLOGICAL CHEMISTRY (1977)
SALOMON, Y, ADVANCES IN CYCLIC NUCLEOTIDE RESEARCH 10: 35 (1979). / ADVANCES IN CYCLIC NUCLEOTIDE RESEARCH (1979)
10.1073/pnas.74.12.5463
10.1016/S0021-9258(18)66747-X
STRYER, L, G-PROTEINS - A FAMILY OF SIGNAL TRANSDUCERS, ANNUAL REVIEW OF CELL BIOLOGY 2: 391 (1986). (10.1146/annurev.cb.02.110186.002135) / ANNUAL REVIEW OF CELL BIOLOGY (1986)
10.1038/330758a0
WEINSTOCK, R.S., OLFACTORY DYSFUNCTION IN HUMANS WITH DEFICIENT GUANINE NUCLEOTIDE-BINDING PROTEIN, NATURE 322: 635 (1986). (10.1038/322635a0) / NATURE (1986)
WOOD, W.I., BASE COMPOSITION-INDEPENDENT HYBRIDIZATION IN TETRAMETHYLAMMONIUM CHLORIDE - A METHOD FOR OLIGONUCLEOTIDE SCREENING OF HIGHLY COMPLEX GENE LIBRARIES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 82: 1585 (1985). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1985)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 11:35 a.m.)
Indexed	3 weeks, 5 days ago (Aug. 2, 2025, 12:54 a.m.)
Issued	36 years, 3 months ago (May 19, 1989)
Published	36 years, 3 months ago (May 19, 1989)
Published Print	36 years, 3 months ago (May 19, 1989)

Funders 0

None

BibTeX

@article{Jones_1989, title={G olf  : an Olfactory Neuron Specific-G Protein Involved in Odorant Signal Transduction}, volume={244}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2499043}, DOI={10.1126/science.2499043}, number={4906}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Jones, David T. and Reed, Randall R.}, year={1989}, month=may, pages={790–795} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        2
      ]
    ],
    "date-time": "2025-08-02T04:54:31Z",
    "timestamp": 1754110471835
  },
  "reference-count": 40,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "4906",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1989,
        5,
        19
      ]
    ]
  },
  "abstract": "<jats:p>\n            Biochemical and electrophysiological studies suggest that odorants induce responses in olfactory sensory neurons via an adenylate cyclase cascade mediated by a G protein. An olfactory-specific guanosine triphosphate (GTP)-binding protein \u03b1 subunit has now been characterized and evidence is presented suggesting that this G protein, termed G\n            <jats:sub>olf</jats:sub>\n            , mediates olfaction. Messenger RNA that encodes G\n            <jats:sub>olf\u03b1</jats:sub>\n            is expressed in olfactory neuroephithelium but not in six other tissues tested. Moreover, within the olfactory epithelium, G\n            <jats:sub>olf\u03b1</jats:sub>\n            appears to be expressed only by the sensory neurons. Specific antisera were used to localize G\n            <jats:sub>olf\u03b1</jats:sub>\n            protein to the sensory apparatus of the receptor neurons. G\n            <jats:sub>olf\u03b1</jats:sub>\n            shares extensive amino acid identity (88 percent) with the stimulatory G protein, G\n            <jats:sub>s\u03b1</jats:sub>\n            . The expression of G\n            <jats:sub>olf\u03b1</jats:sub>\n            in S49 cyc\n            <jats:sup>-</jats:sup>\n            kin\n            <jats:sup>-</jats:sup>\n            cells, a line deficient in endogenous stimulatory G proteins, demonstrates its capacity to stimulate adenylate cyclase in a heterologous system.\n          </jats:p>",
  "DOI": "10.1126/science.2499043",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        10,
        5
      ]
    ],
    "date-time": "2006-10-05T21:21:28Z",
    "timestamp": 1160083288000
  },
  "page": "790-795",
  "source": "Crossref",
  "is-referenced-by-count": 682,
  "title": "G\n            <sub>olf</sub>\n            : an Olfactory Neuron Specific-G Protein Involved in Odorant Signal Transduction",
  "prefix": "10.1126",
  "volume": "244",
  "author": [
    {
      "given": "David T.",
      "family": "Jones",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Howard Hughes Medical Institute, Department of Molecular Biology and Genetic Johns Hopkins School of Medicine, Baltimore, MD 21205."
        }
      ]
    },
    {
      "given": "Randall R.",
      "family": "Reed",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Howard Hughes Medical Institute, Department of Molecular Biology and Genetic Johns Hopkins School of Medicine, Baltimore, MD 21205."
        }
      ]
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_2_1_1_1",
      "doi-asserted-by": "crossref",
      "first-page": "788",
      "DOI": "10.1021/bi00377a020",
      "volume": "26",
      "year": "1987",
      "unstructured": "ANHOLT, RRH, TRANSDUCTION PROTEINS OF OLFACTORY RECEPTOR-CELLS - IDENTIFICATION OF GUANINE-NUCLEOTIDE BINDING-PROTEINS AND PROTEIN-KINASE-C, BIOCHEMISTRY 26: 788 (1987).",
      "journal-title": "BIOCHEMISTRY"
    },
    {
      "key": "e_1_2_1_2_1",
      "first-page": "6779",
      "volume": "81",
      "year": "1984",
      "unstructured": "Baker, T. A., Proceedings of the National Academy of Sciences of the United States of America 81: 6779 (1984).",
      "journal-title": "Proceedings of the National Academy of Sciences of the United States of America"
    },
    {
      "key": "e_1_2_1_3_1",
      "first-page": "93",
      "year": "1982",
      "unstructured": "Beets, M. G. J., Fragrance Chemistry: The Science of Sense and Smell: 93 (1982).",
      "journal-title": "Fragrance Chemistry: The Science of Sense and Smell"
    },
    {
      "key": "e_1_2_1_4_1",
      "first-page": "8893",
      "volume": "83",
      "year": "1986",
      "unstructured": "BRAY, P, HUMAN CDNA CLONES FOR 4 SPECIES OF G-ALPHA-S SIGNAL TRANSDUCTION PROTEIN, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 8893 (1986).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "crossref",
      "first-page": "1493",
      "DOI": "10.1016/S0021-9258(18)89619-3",
      "volume": "260",
      "year": "1985",
      "unstructured": "CERIONE, R.A., SPECIFICITY OF THE FUNCTIONAL INTERACTIONS OF THE BETA-ADRENERGIC-RECEPTOR AND RHODOPSIN WITH GUANINE-NUCLEOTIDE REGULATORY PROTEINS RECONSTITUTED IN PHOSPHOLIPID-VESICLES, JOURNAL OF BIOLOGICAL CHEMISTRY 260: 1493 (1985).",
      "journal-title": "JOURNAL OF BIOLOGICAL CHEMISTRY"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "crossref",
      "first-page": "583",
      "DOI": "10.1210/mend-1-9-583",
      "volume": "1",
      "year": "1987",
      "unstructured": "CULLEN, E.I., BIOSYNTHESIS OF AMIDATED JOINING PEPTIDE FROM PRO-ADRENOCORTICOTROPIN-ENDORPHIN, MOLECULAR ENDOCRINOLOGY 1: 583 (1987).",
      "journal-title": "MOLECULAR ENDOCRINOLOGY"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-2697(83)90418-9"
    },
    {
      "key": "e_1_2_1_8_1",
      "first-page": "3066",
      "volume": "85",
      "year": "1988",
      "unstructured": "FONG, HKW, IDENTIFICATION OF A GTP-BINDING PROTEIN ALPHA-SUBUNIT THAT LACKS AN APPARENT ADP-RIBOSYLATION SITE FOR PERTUSSIS TOXIN, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 3066 (1988).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.56.070187.003151"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "crossref",
      "first-page": "1311",
      "DOI": "10.1177/25.12.336785",
      "volume": "25",
      "year": "1977",
      "unstructured": "GRAZIADEI, GAM, IMMUNOCYTOCHEMISTRY OF OLFACTORY MARKER PROTEIN, JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 25: 1311 (1977).",
      "journal-title": "JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "crossref",
      "first-page": "197",
      "DOI": "10.1007/BF01175561",
      "volume": "8",
      "year": "1979",
      "unstructured": "GRAZIADEI, GAM, NEUROGENESIS AND NEURON REGENERATION IN THE OLFACTORY SYSTEM OF MAMMALS .2. DEGENERATION AND RECONSTITUTION OF THE OLFACTORY SENSORY NEURONS AFTER AXOTOMY, JOURNAL OF NEUROCYTOLOGY 8: 197 (1979).",
      "journal-title": "JOURNAL OF NEUROCYTOLOGY"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "crossref",
      "first-page": "477",
      "DOI": "10.1016/0092-8674(82)90202-1",
      "volume": "28",
      "year": "1982",
      "unstructured": "GREEN, N, IMMUNOGENIC STRUCTURE OF THE INFLUENZA-VIRUS HEMAGGLUTININ, CELL 28: 477 (1982).",
      "journal-title": "CELL"
    },
    {
      "key": "e_1_2_1_13_1",
      "first-page": "435",
      "volume": "9",
      "year": "1984",
      "unstructured": "HALLIDAY, K.R., REGIONAL HOMOLOGY IN GTP-BINDING PROTO-ONCOGENE PRODUCTS AND ELONGATION-FACTORS, JOURNAL OF CYCLIC NUCLEOTIDE AND PROTEIN PHOSPHORYLATION RESEARCH 9: 435 (1984).",
      "journal-title": "JOURNAL OF CYCLIC NUCLEOTIDE AND PROTEIN PHOSPHORYLATION RESEARCH"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "crossref",
      "first-page": "1274",
      "DOI": "10.1126/science.3839937",
      "volume": "229",
      "year": "1985",
      "unstructured": "HARRIS, B.A., MOLECULAR-CLONING OF COMPLEMENTARY-DNA FOR THE ALPHA-SUBUNIT OF THE G-PROTEIN THAT STIMULATES ADENYLATE-CYCLASE, SCIENCE 229: 1274 (1985).",
      "journal-title": "SCIENCE"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "crossref",
      "first-page": "349",
      "DOI": "10.1101/SQB.1988.053.01.042",
      "volume": "53",
      "year": "1988",
      "unstructured": "Jones, D. T., Cold Spring Harbor Symposia on Quantitative Biology 53: 349 (1988).",
      "journal-title": "Cold Spring Harbor Symposia on Quantitative Biology"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)47929-X"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "crossref",
      "first-page": "219",
      "DOI": "10.1089/dna.1988.7.219",
      "volume": "7",
      "year": "1988",
      "unstructured": "KIRSCHMEIER, P.T., DNA 7: 219 (1988).",
      "journal-title": "DNA"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/12.2.857"
    },
    {
      "key": "e_1_2_1_19_1",
      "first-page": "2081",
      "volume": "85",
      "year": "1988",
      "unstructured": "KOZASA, T, ISOLATION AND CHARACTERIZATION OF THE HUMAN GS-ALPHA GENE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 2081 (1988).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "crossref",
      "first-page": "329",
      "DOI": "10.1146/annurev.ne.09.030186.001553",
      "volume": "9",
      "year": "1986",
      "unstructured": "LANCET, D, VERTEBRATE OLFACTORY RECEPTION, ANNUAL REVIEW OF NEUROSCIENCE 9: 329 (1986).",
      "journal-title": "ANNUAL REVIEW OF NEUROSCIENCE"
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.3529395"
    },
    {
      "key": "e_1_2_1_22_1",
      "unstructured": "MACHLIN P unpublished data."
    },
    {
      "key": "e_1_2_1_23_1",
      "first-page": "448",
      "volume": "241",
      "year": "1988",
      "unstructured": "MASTERS, S.B., NATURE 241: 448 (1988).",
      "journal-title": "NATURE"
    },
    {
      "key": "e_1_2_1_24_1",
      "first-page": "47",
      "volume": "1",
      "year": "1986",
      "unstructured": "MASTERS, S.B., FAMILY OF G-PROTEIN ALPHA-CHAINS - AMPHIPATHIC ANALYSIS AND PREDICTED STRUCTURE OF FUNCTIONAL DOMAINS, PROTEIN ENGINEERING 1: 47 (1986).",
      "journal-title": "PROTEIN ENGINEERING"
    },
    {
      "key": "e_1_2_1_25_1",
      "first-page": "5384",
      "volume": "85",
      "year": "1988",
      "unstructured": "MATSUOKA, M, SEQUENCE-ANALYSIS OF CDNA AND GENOMIC DNA FOR A PUTATIVE PERTUSSIS TOXIN-INSENSITIVE GUANINE NUCLEOTIDE-BINDING REGULATORY PROTEIN ALPHA-SUBUNIT, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 5384 (1988).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_26_1",
      "first-page": "265",
      "volume": "83",
      "year": "1986",
      "unstructured": "MUMBY, S.M., ANTISERA OF DESIGNED SPECIFICITY FOR SUBUNITS OF GUANINE NUCLEOTIDE-BINDING REGULATORY PROTEINS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 265 (1986).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/325442a0"
    },
    {
      "key": "e_1_2_1_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/316255a0"
    },
    {
      "key": "e_1_2_1_29_1",
      "first-page": "4947",
      "volume": "83",
      "year": "1986",
      "unstructured": "PACE, U, OLFACTORY GTP-BINDING PROTEIN - SIGNAL-TRANSDUCING POLYPEPTIDE OF VERTEBRATE CHEMOSENSORY NEURONS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 4947 (1986).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_30_1",
      "first-page": "3050",
      "volume": "82",
      "year": "1985",
      "unstructured": "PEVSNER, J, ISOLATION AND CHARACTERIZATION OF AN OLFACTORY RECEPTOR PROTEIN FOR ODORANT PYRAZINES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 82: 3050 (1985).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_31_1",
      "first-page": "4412",
      "volume": "77",
      "year": "1980",
      "unstructured": "Rhein, L. D., Proceedings of the National Academy of Sciences of the United States of America 77: 4412 (1980).",
      "journal-title": "Proceedings of the National Academy of Sciences of the United States of America"
    },
    {
      "key": "e_1_2_1_32_1",
      "doi-asserted-by": "crossref",
      "first-page": "9587",
      "DOI": "10.1016/S0021-9258(18)67553-2",
      "volume": "261",
      "year": "1986",
      "unstructured": "ROBISHAW, J.D., MOLECULAR-BASIS FOR 2 FORMS OF THE G-PROTEIN THAT STIMULATES ADENYLATE-CYCLASE, JOURNAL OF BIOLOGICAL CHEMISTRY 261: 9587 (1986).",
      "journal-title": "JOURNAL OF BIOLOGICAL CHEMISTRY"
    },
    {
      "key": "e_1_2_1_33_1",
      "doi-asserted-by": "crossref",
      "first-page": "5761",
      "DOI": "10.1016/S0021-9258(17)40089-5",
      "volume": "252",
      "year": "1977",
      "unstructured": "ROSS, E.M., RELATIONSHIP BETWEEN BETA-ADRENERGIC-RECEPTOR AND ADENYLATE-CYCLASE - STUDIES OF LIGAND-BINDING AND ENZYME-ACTIVITY IN PURIFIED MEMBRANES OF S49 LYMPHOMA-CELLS, JOURNAL OF BIOLOGICAL CHEMISTRY 252: 5761 (1977).",
      "journal-title": "JOURNAL OF BIOLOGICAL CHEMISTRY"
    },
    {
      "key": "e_1_2_1_34_1",
      "first-page": "35",
      "volume": "10",
      "year": "1979",
      "unstructured": "SALOMON, Y, ADVANCES IN CYCLIC NUCLEOTIDE RESEARCH 10: 35 (1979).",
      "journal-title": "ADVANCES IN CYCLIC NUCLEOTIDE RESEARCH"
    },
    {
      "key": "e_1_2_1_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.74.12.5463"
    },
    {
      "key": "e_1_2_1_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)66747-X"
    },
    {
      "key": "e_1_2_1_37_1",
      "doi-asserted-by": "crossref",
      "first-page": "391",
      "DOI": "10.1146/annurev.cb.02.110186.002135",
      "volume": "2",
      "year": "1986",
      "unstructured": "STRYER, L, G-PROTEINS - A FAMILY OF SIGNAL TRANSDUCERS, ANNUAL REVIEW OF CELL BIOLOGY 2: 391 (1986).",
      "journal-title": "ANNUAL REVIEW OF CELL BIOLOGY"
    },
    {
      "key": "e_1_2_1_38_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/330758a0"
    },
    {
      "key": "e_1_2_1_39_1",
      "doi-asserted-by": "crossref",
      "first-page": "635",
      "DOI": "10.1038/322635a0",
      "volume": "322",
      "year": "1986",
      "unstructured": "WEINSTOCK, R.S., OLFACTORY DYSFUNCTION IN HUMANS WITH DEFICIENT GUANINE NUCLEOTIDE-BINDING PROTEIN, NATURE 322: 635 (1986).",
      "journal-title": "NATURE"
    },
    {
      "key": "e_1_2_1_40_1",
      "first-page": "1585",
      "volume": "82",
      "year": "1985",
      "unstructured": "WOOD, W.I., BASE COMPOSITION-INDEPENDENT HYBRIDIZATION IN TETRAMETHYLAMMONIUM CHLORIDE - A METHOD FOR OLIGONUCLEOTIDE SCREENING OF HIGHLY COMPLEX GENE LIBRARIES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 82: 1585 (1985).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.2499043",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        12
      ]
    ],
    "date-time": "2024-01-12T16:35:40Z",
    "timestamp": 1705077340000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.2499043"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1989,
        5,
        19
      ]
    ]
  },
  "references-count": 40,
  "journal-issue": {
    "issue": "4906",
    "published-print": {
      "date-parts": [
        [
          1989,
          5,
          19
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.2499043"
  ],
  "URL": "http://dx.doi.org/10.1126/science.2499043",
  "relation": {},
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1989,
        5,
        19
      ]
    ]
  }
}