DOI: 10.1126/science.2497519. Mouse Embryonic Stem Cells and Reporter Constructs to Detect Developmentally Regulated Genes

Mouse Embryonic Stem Cells and Reporter Constructs to Detect Developmentally Regulated Genes

10.1126/science.2497519

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A strategy was devised for identifying regions of the mouse genome that are transcriptionally active in a temporally and spatially restricted manner during development. The approach is based on the introduction into embryonic stem cells of two types of lacZ reporter constructs that can be activated by flanking mouse genomic sequences. Embryonic stem cells containing the lacZ constructs were used to produce chimaeric mice. Developmental regulation of lacZ expression occurred at a high frequency. Molecular cloning of the flanking endogenous genes and introduction of these potential insertional mutations into the mouse germ line should provide an efficient means of identifying and mutating novel genes important for the control of mammalian development.

Bibliography

Gossler, A., Joyner, A. L., Rossant, J., & Skarnes, W. C. (1989). Mouse Embryonic Stem Cells and Reporter Constructs to Detect Developmentally Regulated Genes. Science, 244(4903), 463â465.

Authors 4

Achim Gossler (first)
Alexandra L. Joyner (additional)
Janet Rossant (additional)
William C. Skarnes (additional)

References 19 Referenced 390

ALLEN, N.D., TRANSGENES AS PROBES FOR ACTIVE CHROMOSOMAL DOMAINS IN MOUSE DEVELOPMENT, NATURE 333: 852 (1988). (10.1038/333852a0) / NATURE (1988)
Beddington, S. P., Development 106: 37 (1989). (10.1242/dev.106.1.37) / Development (1989)
DOETSCHMAN, T.C., THE INVITRO DEVELOPMENT OF BLASTOCYST-DERIVED EMBRYONIC STEM-CELL LINES - FORMATION OF VISCERAL YOLK-SAC, BLOOD ISLANDS AND MYOCARDIUM, JOURNAL OF EMBRYOLOGY AND EXPERIMENTAL MORPHOLOGY 87: 27 (1985). / JOURNAL OF EMBRYOLOGY AND EXPERIMENTAL MORPHOLOGY (1985)
EVANS, M.J., ESTABLISHMENT IN CULTURE OF PLURIPOTENTIAL CELLS FROM MOUSE EMBRYOS, NATURE 292: 154 (1981). (10.1038/292154a0) / NATURE (1981)
GAL, S, METHODS IN ENZYMOLOGY 151: 104 (1987). (10.1016/S0076-6879(87)51011-4) / METHODS IN ENZYMOLOGY (1987)
10.1073/pnas.83.23.9065
GOSSLER A unpublished data.
JOYNER, A.L., EN-1 AND EN-2, 2 MOUSE GENES WITH SEQUENCE HOMOLOGY TO THE DROSOPHILA ENGRAILED GENE - EXPRESSION DURING EMBRYOGENESIS, GENES & DEVELOPMENT 1: 29 (1987). (10.1101/gad.1.1.29) / GENES & DEVELOPMENT (1987)
Joyner, A. L., Nature 338: 153 (1989). (10.1038/338153a0) / Nature (1989)
KOTHARY, R, A TRANSGENE CONTAINING LACZ INSERTED INTO THE DYSTONIA LOCUS IS EXPRESSED IN NEURAL-TUBE, NATURE 335: 435 (1988). (10.1038/335435a0) / NATURE (1988)
MAGLI, M.C., MODULATION OF GENE-EXPRESSION IN MULTIPLE HEMATOPOIETIC-CELL LINEAGES FOLLOWING RETROVIRAL VECTOR GENE-TRANSFER, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 84: 789 (1987). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1987)
MARTIN, G.R., ISOLATION OF A PLURIPOTENT CELL-LINE FROM EARLY MOUSE EMBRYOS CULTURED IN MEDIUM CONDITIONED BY TERATOCARCINOMA STEM-CELLS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 78: 7634 (1981). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1981)
10.1073/pnas.84.24.9123
10.1073/pnas.84.1.156
ROBERTSON, E, GERM-LINE TRANSMISSION OF GENES INTRODUCED INTO CULTURED PLURIPOTENTIAL CELLS BY RETROVIRAL VECTOR, NATURE 323: 445 (1986). (10.1038/323445a0) / NATURE (1986)
ROSSANT, J, INTERSPECIFIC CELL MARKERS AND LINEAGE IN MAMMALS, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES 312: 91 (1985). / PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES (1985)
10.1002/j.1460-2075.1986.tb04620.x
SEED, B, PURIFICATION OF GENOMIC SEQUENCES FROM BACTERIOPHAGE LIBRARIES BY RECOMBINATION AND SELECTION INVIVO, NUCLEIC ACIDS RESEARCH 11: 2427 (1983). (10.1093/nar/11.8.2427) / NUCLEIC ACIDS RESEARCH (1983)
SKARNES W.C. unpublished data.

Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 11:20 a.m.)
Indexed	1 day, 11 hours ago (Sept. 4, 2025, 9:49 a.m.)
Issued	36 years, 4 months ago (April 28, 1989)
Published	36 years, 4 months ago (April 28, 1989)
Published Print	36 years, 4 months ago (April 28, 1989)

Funders 0

None

BibTeX

@article{Gossler_1989, title={Mouse Embryonic Stem Cells and Reporter Constructs to Detect Developmentally Regulated Genes}, volume={244}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2497519}, DOI={10.1126/science.2497519}, number={4903}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Gossler, Achim and Joyner, Alexandra L. and Rossant, Janet and Skarnes, William C.}, year={1989}, month=apr, pages={463–465} }

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