Specific Interactions in RNA Enzyme-Substrate Complexes
10.1126/science.2480641
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Analysis of crosslinked complexes of M1 RNA, the catalytic RNA subunit of ribonuclease P from Escherichia coli , and transfer RNA precursor substrates has led to the identification of regions in the enzyme and in the substrate that are in close physical proximity to each other. The nucleotide in M1 RNA, residue C92, which participates in a crosslink with the substrate was deleted and the resulting mutant M1 RNA was shown to cleave substrates lacking the 3′ terminal CCAUCA sequence at sites several nucleotides away from the normal site of cleavage. The presence or absence of the 3′ terminal CCAUCA sequence in transfer RNA precursor substrates markedly affects the way in which these substrates interact with the catalytic RNA in the enzyme-substrate complex. The contacts between wild-type M1 RNA and its substrate are in a region that resembles part of the transfer RNA "E" (exit) site in 23 S ribosomal RNA. These data demonstrate that in RNA's with very different cellular functions, there are domains with similar structural and functional properties and that there is a nucleotide in M1 RNA that affects the site of cleavage by the enzyme.

Bibliography

Guerrier-Takada, C., Lumelsky, N., & Altman, S. (1989). Specific Interactions in RNA Enzyme-Substrate Complexes. Science, 246(4937), 1578–1584.

Authors 3
  1. Cecilia Guerrier-Takada (first)
  2. Nadya Lumelsky (additional)
  3. Sidney Altman (additional)
References 34 Referenced 81
  1. ALTMAN, S, RIBONUCLEASE-P - AN ENZYME WITH A CATALYTIC RNA SUBUNIT, ADVANCES IN ENZYMOLOGY AND RELATED AREAS OF MOLECULAR BIOLOGY 62: 1 (1989). / ADVANCES IN ENZYMOLOGY AND RELATED AREAS OF MOLECULAR BIOLOGY (1989)
  2. Altman, S., Molecular Biology of RNA: 3 (1987). / Molecular Biology of RNA (1987)
  3. BBRANCH, A.D., SCIENCE 243: 649 (1989). (10.1126/science.2492676) / SCIENCE (1989)
  4. BOTHWELL, ALM, RIBONUCLEASE P SUBSTRATE-SPECIFICITY - CLEAVAGE OF A BACTERIOPHAGE PHI-80-INDUCED RNA, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 73: 1912 (1976). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1976)
  5. BRANCH, A.D., ANALYSIS OF ULTRAVIOLET-INDUCED RNA RNA CROSS-LINKS - A MEANS FOR PROBING RNA STRUCTURE-FUNCTION-RELATIONSHIPS, METHODS IN ENZYMOLOGY 180: 418 (1989). (10.1016/0076-6879(89)80115-6) / METHODS IN ENZYMOLOGY (1989)
  6. BRANCH, A.D., UNUSUAL PROPERTIES OF 2 BRANCHED RNAS WITH CIRCULAR AND LINEAR COMPONENTS, NUCLEIC ACIDS RESEARCH 13: 4889 (1985). (10.1093/nar/13.13.4889) / NUCLEIC ACIDS RESEARCH (1985)
  7. BURKARD, U, PROCESSING OF HISTIDINE TRANSFER-RNA PRECURSORS - ABNORMAL CLEAVAGE SITE FOR RNASE-P, JOURNAL OF BIOLOGICAL CHEMISTRY 263: 2447 (1988). (10.1016/S0021-9258(18)69227-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1988)
  8. CARRARA, G, SITE SELECTION BY XENOPUS-LAEVIS RNAASE-P, CELL 58: 37 (1989). (10.1016/0092-8674(89)90400-5) / CELL (1989)
  9. 10.1126/science.2438771
  10. CLARK, J.M., NUCLEIC ACIDS RESEARCH 16: 9977 (1988). / NUCLEIC ACIDS RESEARCH (1988)
  11. Deutscher, M. P., Enzymes of Nucleic Acid Synthesis and Modifications: 159 (1983). / Enzymes of Nucleic Acid Synthesis and Modifications (1983)
  12. GOLD, H.A., RECONSTITUTION OF RNASE-P ACTIVITY USING INACTIVE SUBUNITS FROM ESCHERICHIA-COLI AND HELA-CELLS, CELL 44: 243 (1986). (10.1016/0092-8674(86)90758-0) / CELL (1986)
  13. 10.1016/0092-8674(83)90117-4
  14. GUERRIERTAKADA, C, CLEAVAGE OF TRANSFER-RNA PRECURSORS BY THE RNA SUBUNIT OF ESCHERICHIA-COLI RIBONUCLEASE-P (M1 RNA) IS INFLUENCED BY 3'-PROXIMAL CCA IN THE SUBSTRATES, CELL 38: 219 (1984). (10.1016/0092-8674(84)90543-9) / CELL (1984)
  15. GUERRIERTAKADA, C, NOVEL REACTIONS OF RNAASE-P WITH A TRANSFER-RNA-LIKE STRUCTURE IN TURNIP YELLOW MOSAIC-VIRUS RNA, CELL 53: 267 (1988). (10.1016/0092-8674(88)90388-1) / CELL (1988)
  16. GUERRIERTAKADA C unpublished data.
  17. JAMES, B.D., THE SECONDARY STRUCTURE OF RIBONUCLEASE-P RNA, THE CATALYTIC ELEMENT OF A RIBONUCLEOPROTEIN ENZYME, CELL 52: 19 (1988). (10.1016/0092-8674(88)90527-2) / CELL (1988)
  18. KIRSEBOM, L.A., DIFFERENTIAL-EFFECTS OF MUTATIONS IN THE PROTEIN AND RNA MOIETIES OF RNASE-P ON THE EFFICIENCY OF SUPPRESSION BY VARIOUS TRANSFER-RNA SUPPRESSORS, JOURNAL OF MOLECULAR BIOLOGY 204: 879 (1988). (10.1016/0022-2836(88)90048-4) / JOURNAL OF MOLECULAR BIOLOGY (1988)
  19. KIRSEBOM L unpublished data.
  20. KRAMER, W, THE GAPPED DUPLEX DNA APPROACH TO OLIGONUCLEOTIDE-DIRECTED MUTATION CONSTRUCTION, NUCLEIC ACIDS RESEARCH 12: 9441 (1984). (10.1093/nar/12.24.9441) / NUCLEIC ACIDS RESEARCH (1984)
  21. KRUPP, G, EMBO J 5: 1967 (1986). (10.1002/j.1460-2075.1986.tb04451.x) / EMBO J (1986)
  22. LUMELSKY, N, SELECTION AND CHARACTERIZATION OF RANDOMLY PRODUCED MUTANTS IN THE GENE CODING FOR M1-RNA, JOURNAL OF MOLECULAR BIOLOGY 202: 443 (1988). (10.1016/0022-2836(88)90277-X) / JOURNAL OF MOLECULAR BIOLOGY (1988)
  23. LUMELSKY N unpublished data.
  24. Maniatis T. Molecular Cloning: A Laboratory Manual (1982).
  25. MASIAKOWSKI, P, SEPARATION OF FUNCTIONALLY DISTINCT REGIONS OF A MACROMOLECULAR SUBSTRATE - STIMULATION OF TRANSFER-RNA NUCLEOTIDYLTRANSFERASE BY A NONREACTING FRAGMENT OF TRANSFER-RNA, JOURNAL OF BIOLOGICAL CHEMISTRY 254: 2585 (1979). (10.1016/S0021-9258(17)30110-2) / JOURNAL OF BIOLOGICAL CHEMISTRY (1979)
  26. 10.1016/0092-8674(89)90128-1
  27. MOAZED, D, RAPID CHEMICAL PROBING OF CONFORMATION IN 16-S RIBOSOMAL-RNA AND 30-S RIBOSOMAL-SUBUNITS USING PRIMER EXTENSION, JOURNAL OF MOLECULAR BIOLOGY 187: 399 (1986). (10.1016/0022-2836(86)90441-9) / JOURNAL OF MOLECULAR BIOLOGY (1986)
  28. 10.1126/science.3122322
  29. RENAUD, M, CONFORMATIONAL ACTIVATION OF THE YEAST PHENYLALANYL-TRANSFER RNA-SYNTHETASE CATALYTIC SITE INDUCED BY TRANSFER RNAPHE INTERACTION - TRIGGERING OF ADENOSINE OR CPCPA TRINUCLEOSIDE DIPHOSPHATE AMINOACYLATION UPON BINDING OF TRANSFER RNAPHE LACKING THESE RESIDUES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 78: 1606 (1981). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1981)
  30. SHIRAISHI, H, FUNCTIONAL DOMAINS OF THE RNA COMPONENT OF RIBONUCLEASE-P REVEALED BY CHEMICAL PROBING OF MUTANT RNAS, EMBO JOURNAL 7: 3817 (1988). (10.1002/j.1460-2075.1988.tb03266.x) / EMBO JOURNAL (1988)
  31. TABLER, M, MOLECULAR-CLONING OF POTATO SPINDLE TUBER VIROID (PSTV) CDNA SYNTHESIZED BY ENZYMATIC ELONGATION OF PSTV-SPECIFIC DNA PRIMERS - A GENERAL STRATEGY FOR VIROID CLONING, BIOSCIENCE REPORTS 5: 143 (1985). (10.1007/BF01117061) / BIOSCIENCE REPORTS (1985)
  32. VIOQUE, A, PROTEIN-SYNTHESIS INHIBITORS AND CATALYTIC RNA - EFFECT OF PUROMYCIN ON TRANSFER-RNA PRECURSOR PROCESSING BY THE RNA COMPONENT OF ESCHERICHIA-COLI RNASE P, FEBS LETTERS 246: 137 (1989). (10.1016/0014-5793(89)80269-8) / FEBS LETTERS (1989)
  33. VIOQUE, A, PROTEIN RNA INTERACTIONS IN THE RNASE-P HOLOENZYME FROM ESCHERICHIA-COLI, JOURNAL OF MOLECULAR BIOLOGY 202: 835 (1988). (10.1016/0022-2836(88)90562-1) / JOURNAL OF MOLECULAR BIOLOGY (1988)
  34. 10.1093/nar/10.20.6487
Dates
Type When
Created 18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited 1 year, 7 months ago (Jan. 12, 2024, 9:50 a.m.)
Indexed 11 months, 2 weeks ago (Sept. 12, 2024, 2:32 p.m.)
Issued 35 years, 8 months ago (Dec. 22, 1989)
Published 35 years, 8 months ago (Dec. 22, 1989)
Published Print 35 years, 8 months ago (Dec. 22, 1989)
Funders 0

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@article{Guerrier_Takada_1989, title={Specific Interactions in RNA Enzyme-Substrate Complexes}, volume={246}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2480641}, DOI={10.1126/science.2480641}, number={4937}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Guerrier-Takada, Cecilia and Lumelsky, Nadya and Altman, Sidney}, year={1989}, month=dec, pages={1578–1584} }