DOI: 10.1126/science.2460923. A Pituitary <i>N</i> -Acetylgalactosamine Transferase That Specifically Recognizes Glycoprotein Hormones

A Pituitary N -Acetylgalactosamine Transferase That Specifically Recognizes Glycoprotein Hormones

10.1126/science.2460923

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The glycoprotein hormones lutropin (LH) and follitropin (FSH), which have common α-subunits but hormone-specific β-subunits, are both synthesized in the gonadotroph. However, they bear Asn-linked oligosaccharides that differ in structure. Those on LH terminate with the sequence SO 4 -4GalNAcβ1→4GlcNAcβ1→2Manα, whereas those on FSH terminate with the sequence sialic acidα-Galβ1→4GlcNAcβ1→2Manα. A GalNAc-transferase was identified in bovine pituitary membranes that recognizes features of the α-subunit peptide and adds GalNAc to its oligosaccharides with an apparent Michaelis constant of 25 micromolar. The different patterns of glycosylation for LH and FSH indicate that access to the protein recognition marker on the α-subunit is modulated by the associated β-subunit. The tightly regulated synthesis of sulfated and sialylated oligosaccharides on the pituitary glycoprotein hormones suggests these oligosaccharides have an important biological role.

Bibliography

Smith, P. L., & Baenziger, J. U. (1988). A Pituitary N -Acetylgalactosamine Transferase That Specifically Recognizes Glycoprotein Hormones. Science, 242(4880), 930â933.

Authors 2

Peter L. Smith (first)
Jacques U. Baenziger (additional)

References 20 Referenced 82

BAENZIGER, J.U., PITUITARY GLYCOPROTEIN HORMONE OLIGOSACCHARIDES - STRUCTURE, SYNTHESIS AND FUNCTION OF THE ASPARAGINE-LINKED OLIGOSACCHARIDES ON LUTROPIN, FOLLITROPIN AND THYROTROPIN, BIOCHIMICA ET BIOPHYSICA ACTA 947: 287 (1988). (10.1016/0304-4157(88)90012-3) / BIOCHIMICA ET BIOPHYSICA ACTA (1988)
BAENZIGER, J.U., PLASMA PROTEINS 4: 272 (1984). / PLASMA PROTEINS (1984)
CHILDS, G.V., AN IMMUNOCYTOCHEMIST VIEW OF GONADOTROPIN STORAGE IN THE ADULT MALE-RAT - CYTOCHEMICAL AND MORPHOLOGICAL HETEROGENEITY IN SERIALLY SECTIONED GONADOTROPES, AMERICAN JOURNAL OF ANATOMY 158: 397 (1980). (10.1002/aja.1001580403) / AMERICAN JOURNAL OF ANATOMY (1980)
CHILDS, G, HORMONAL CONTROL HYP: 181 (1984). / HORMONAL CONTROL HYP (1984)
ENDO, Y, STRUCTURES OF THE ASPARAGINE-LINKED SUGAR CHAINS OF HUMAN CHORIONIC-GONADOTROPIN, JOURNAL OF BIOCHEMISTRY 85: 669 (1979). / JOURNAL OF BIOCHEMISTRY (1979)
GREEN, E.D., LECTIN AFFINITY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - INTERACTIONS OF N-GLYCANASE-RELEASED OLIGOSACCHARIDES WITH LEUKOAGGLUTINATING PHYTOHEMAGGLUTININ, CONCANAVALIN-A, DATURA-STRAMONIUM AGGLUTININ, AND VICIA-VILLOSA AGGLUTININ, ANALYTICAL BIOCHEMISTRY 167: 62 (1987). (10.1016/0003-2697(87)90134-5) / ANALYTICAL BIOCHEMISTRY (1987)
GREEN, E.D., STRUCTURAL ELUCIDATION OF THE DISULFATED OLIGOSACCHARIDE FROM BOVINE LUTROPIN, JOURNAL OF BIOLOGICAL CHEMISTRY 260: 15623 (1985). (10.1016/S0021-9258(17)36304-4) / JOURNAL OF BIOLOGICAL CHEMISTRY (1985)
GREEN, E.D., LECTIN AFFINITY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - INTERACTIONS OF N-GLYCANASE-RELEASED OLIGOSACCHARIDES WITH RICINUS-COMMUNIS AGGLUTININ-I AND RICINUS-COMMUNIS AGGLUTININ-II, JOURNAL OF BIOLOGICAL CHEMISTRY 262: 12030 (1987). (10.1016/S0021-9258(18)45313-6) / JOURNAL OF BIOLOGICAL CHEMISTRY (1987)
GREEN, E.D., ASPARAGINE-LINKED OLIGOSACCHARIDES ON LUTROPIN, FOLLITROPIN, AND THYROTROPIN .1. STRUCTURAL ELUCIDATION OF THE SULFATED AND SIALYLATED OLIGOSACCHARIDES ON BOVINE, OVINE, AND HUMAN PITUITARY GLYCOPROTEIN HORMONES, JOURNAL OF BIOLOGICAL CHEMISTRY 263: 25 (1988). (10.1016/S0021-9258(19)57351-3) / JOURNAL OF BIOLOGICAL CHEMISTRY (1988)
GREEN, E.D., ASPARAGINE-LINKED OLIGOSACCHARIDES ON LUTROPIN, FOLLITROPIN, AND THYROTROPIN .2. DISTRIBUTIONS OF SULFATED AND SIALYLATED OLIGOSACCHARIDES ON BOVINE, OVINE, AND HUMAN PITUITARY GLYCOPROTEIN HORMONES, JOURNAL OF BIOLOGICAL CHEMISTRY 263: 36 (1988). (10.1016/S0021-9258(19)57352-5) / JOURNAL OF BIOLOGICAL CHEMISTRY (1988)
GREEN, E.D., DIFFERENTIAL PROCESSING OF ASN-LINKED OLIGOSACCHARIDES ON PITUITARY GLYCOPROTEIN HORMONES - IMPLICATIONS FOR BIOLOGIC FUNCTION, MOLECULAR AND CELLULAR BIOCHEMISTRY 72: 81 (1986). / MOLECULAR AND CELLULAR BIOCHEMISTRY (1986)
GREEN, E.D., SULFATION OF LUTROPIN OLIGOSACCHARIDES WITH A CELL-FREE SYSTEM, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 81: 5320 (1984). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1984)
KESSLER, M.J., STRUCTURES OF N-GLYCOSIDIC CARBOHYDRATE UNITS OF HUMAN CHORIONIC-GONADOTROPIN, JOURNAL OF BIOLOGICAL CHEMISTRY 254: 7901 (1979). (10.1016/S0021-9258(18)36031-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1979)
KORNFELD, S, TRAFFICKING OF LYSOSOMAL-ENZYMES, FASEB JOURNAL 1: 462 (1987). (10.1096/fasebj.1.6.3315809) / FASEB JOURNAL (1987)
MELLIS, S.J., SIZE FRACTIONATION OF ANIONIC OLIGOSACCHARIDES AND GLYCOPEPTIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, ANALYTICAL BIOCHEMISTRY 134: 442 (1983). (10.1016/0003-2697(83)90320-2) / ANALYTICAL BIOCHEMISTRY (1983)
PIERCE, J.G., GLYCOPROTEIN HORMONES - STRUCTURE AND FUNCTION, ANNUAL REVIEW OF BIOCHEMISTRY 50: 465 (1981). (10.1146/annurev.bi.50.070181.002341) / ANNUAL REVIEW OF BIOCHEMISTRY (1981)
REITMAN, M.L., LYSOSOMAL-ENZYME TARGETING - N-ACETYLGLUCOSAMINYLPHOSPHOTRANSFERASE SELECTIVELY PHOSPHORYLATES NATIVE LYSOSOMAL-ENZYMES, JOURNAL OF BIOLOGICAL CHEMISTRY 256: 1977 (1981). / JOURNAL OF BIOLOGICAL CHEMISTRY (1981)
SAIRAM, M.R., HORMONAL PROTEINS PE 11: 1 (1983). / HORMONAL PROTEINS PE (1983)
SKELTON T unpublished data.
TAI, T, ASPARAGINE-LINKED OLIGOSACCHARIDE CHAINS OF IGG - REVISED STRUCTURE, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 65: 968 (1975). (10.1016/S0006-291X(75)80480-3) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1975)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 11:45 a.m.)
Indexed	1 year ago (Aug. 16, 2024, 1:09 a.m.)
Issued	36 years, 9 months ago (Nov. 11, 1988)
Published	36 years, 9 months ago (Nov. 11, 1988)
Published Print	36 years, 9 months ago (Nov. 11, 1988)

Funders 0

None

BibTeX

@article{Smith_1988, title={A Pituitary N -Acetylgalactosamine Transferase That Specifically Recognizes Glycoprotein Hormones}, volume={242}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2460923}, DOI={10.1126/science.2460923}, number={4880}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Smith, Peter L. and Baenziger, Jacques U.}, year={1988}, month=nov, pages={930–933} }

JSON

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