DOI: 10.1126/science.2459773. Changing the Acceptor Identity of a Transfer RNA by Altering Nucleotides in a "Variable Pocket"

Changing the Acceptor Identity of a Transfer RNA by Altering Nucleotides in a "Variable Pocket"

10.1126/science.2459773

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The specificity of tRNA Arg (arginine transfer RNA) for aminoacylation (its acceptor identity) were first identified by computer analysis and then examined with amber suppressor tRNAs in Escherichia coli . On replacing two nucleotides in tRNA Phe (phenylalanine transfer RNA) with the corresponding nucleotides from tRNA Arg , the acceptor identity of the resulting tRNA was changed to that of tRNA Arg . The nucleotides used in the identity transformation occupy a "variable pocket" structure on the surface of the tRNA molecule where two single-stranded loop segments interact. The middle nucleotide in the anticodon also probably contributes to the interaction, since an amber suppressor of tRNA Arg had an acceptor identity for lysine as well as arginine.

Bibliography

McClain, W. H., & Foss, K. (1988). Changing the Acceptor Identity of a Transfer RNA by Altering Nucleotides in a âVariable Pocket.â Science, 241(4874), 1804â1807.

Authors 2

William H. McClain (first)
K. Foss (additional)

References 24 Referenced 84

ATILGAN, T, A STATISTICAL-METHOD FOR CORRELATING TRANSFER-RNA SEQUENCE WITH AMINO-ACID SPECIFICITY, NUCLEIC ACIDS RESEARCH 14: 375 (1986). (10.1093/nar/14.1.375) / NUCLEIC ACIDS RESEARCH (1986)
CELIS, J. E., NATURE-NEW BIOLOGY 244: 261 (1973). (10.1038/newbio244261a0) / NATURE-NEW BIOLOGY (1973)
CHAKRABURTTY, K, EFFECT OF SODIUM BISULFITE MODIFICATION ON ARGININE ACCEPTANCE OF ESCHERICHIA-COLI TRANSFER-RNA (ARG), NUCLEIC ACIDS RESEARCH 2: 1793 (1975). (10.1093/nar/2.10.1793) / NUCLEIC ACIDS RESEARCH (1975)
CHAKRABURTTY, K, PRIMARY STRUCTURE OF TRANSFER-RNA (ARG)II OF ESCHERICHIA-COLI-B, NUCLEIC ACIDS RESEARCH 2: 1787 (1975). (10.1093/nar/2.10.1787) / NUCLEIC ACIDS RESEARCH (1975)
CROTHERS, D.M., IS THERE A DISCRIMINATOR SITE IN TRANSFER-RNA, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 69: 3063 (1972). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1972)
HOU, Y.M., A SIMPLE STRUCTURAL FEATURE IS A MAJOR DETERMINANT OF THE IDENTITY OF A TRANSFER-RNA, NATURE 333: 140 (1988). (10.1038/333140a0) / NATURE (1988)
LADNER, J.E., STRUCTURE OF YEAST PHENYLALANINE TRANSFER-RNA AT 2.5 A RESOLUTION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 72: 4414 (1975). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1975)
LORBER, B, CRYSTALLIZATION OF A TRANSFER-RNA AMINOACYL TRANSFER RNA-SYNTHETASE COMPLEX - CHARACTERIZATION AND 1ST CRYSTALLOGRAPHIC DATA, JOURNAL OF BIOLOGICAL CHEMISTRY 258: 8429 (1983). (10.1016/S0021-9258(20)82082-1) / JOURNAL OF BIOLOGICAL CHEMISTRY (1983)
McClain, W. H., Journal of Molecular Biology 203: 549 (1988). (10.1016/0022-2836(88)90191-X) / Journal of Molecular Biology (1988)
MCCLAIN, W.H., DIFFERENCES BETWEEN TRANSFER-RNA MOLECULES, JOURNAL OF MOLECULAR BIOLOGY 194: 635 (1987). (10.1016/0022-2836(87)90240-3) / JOURNAL OF MOLECULAR BIOLOGY (1987)
MCCLAIN, W.H., NUCLEOTIDES THAT CONTRIBUTE TO THE IDENTITY OF ESCHERICHIA-COLI TRANSFER RNAPHE, JOURNAL OF MOLECULAR BIOLOGY 202: 697 (1988). (10.1016/0022-2836(88)90551-7) / JOURNAL OF MOLECULAR BIOLOGY (1988)
MCCLAIN, W.H., VARIANTS IN CLONES OF GENE-MACHINE-SYNTHESIZED OLIGODEOXYNUCLEOTIDES, NUCLEIC ACIDS RESEARCH 14: 6770 (1986). (10.1093/nar/14.16.6770) / NUCLEIC ACIDS RESEARCH (1986)
10.1126/science.2443980
10.1126/science.2452483
MURAO, K, PRIMARY SEQUENCE OF ARGININE TRANSFER-RNA FROM ESCHERICHIA-COLI, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 47: 1332 (1972). (10.1016/0006-291X(72)90218-5) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1972)
NORMANLY, J, CHANGING THE IDENTITY OF A TRANSFER-RNA, NATURE 321: 213 (1986). (10.1038/321213a0) / NATURE (1986)
NORMANLY, J, CONSTRUCTION OF 2 ESCHERICHIA-COLI AMBER SUPPRESSOR GENES - TRANSFER-RNA CUA-PHE AND TRANSFER-RNA CUA-CYS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 6548 (1986). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1986)
Perona, J., Journal of Molecular Biology 202: 121 (1988). (10.1016/0022-2836(88)90524-4) / Journal of Molecular Biology (1988)
PRATHER, N.E., NUCLEOTIDE SUBSTITUTION IN THE AMINO-ACID ACCEPTOR STEM OF LYSINE TRANSFER-RNA CAUSES MISSENSE SUPPRESSION, JOURNAL OF MOLECULAR BIOLOGY 172: 177 (1984). (10.1016/S0022-2836(84)80036-4) / JOURNAL OF MOLECULAR BIOLOGY (1984)
Rogers, M. J., Proceedings of the National Academy of Sciences of the United States of America 85: 6627 (1988). / Proceedings of the National Academy of Sciences of the United States of America (1988)
SAMPSON, J.R., BIOCHEMICAL AND PHYSICAL CHARACTERIZATION OF AN UNMODIFIED YEAST PHENYLALANINE TRANSFER-RNA TRANSCRIBED INVITRO, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 1033 (1988). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1988)
SCHULMAN, L.H., INVITRO CONVERSION OF A METHIONINE TO A GLUTAMINE-ACCEPTOR TRANSFER-RNA, BIOCHEMISTRY 24: 7309 (1985). (10.1021/bi00346a043) / BIOCHEMISTRY (1985)
SPRINZL, M, NUCLEIC ACIDS RESEARCH 15: R53 (1987). (10.1093/nar/15.suppl.r53) / NUCLEIC ACIDS RESEARCH (1987)
YARUS, M, NATURE-NEW BIOLOGY 239: 106 (1972). (10.1038/newbio239106a0) / NATURE-NEW BIOLOGY (1972)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 10:40 a.m.)
Indexed	2 months, 3 weeks ago (June 9, 2025, 5:27 a.m.)
Issued	36 years, 11 months ago (Sept. 30, 1988)
Published	36 years, 11 months ago (Sept. 30, 1988)
Published Print	36 years, 11 months ago (Sept. 30, 1988)

Funders 0

None

BibTeX

@article{McClain_1988, title={Changing the Acceptor Identity of a Transfer RNA by Altering Nucleotides in a “Variable Pocket”}, volume={241}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2459773}, DOI={10.1126/science.2459773}, number={4874}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={McClain, William H. and Foss, K.}, year={1988}, month=sep, pages={1804–1807} }

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