DOI: 10.1126/science.2436299. The α Subunit of the GTP Binding Protein G <sub>k</sub> Opens Atrial Potassium Channels

The α Subunit of the GTP Binding Protein G k Opens Atrial Potassium Channels

10.1126/science.2436299

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Guanine nucleotide binding (G) proteins (subunit composition αβγ) dissociate on activation with guanosine triphosphate (GTP) analogs and magnesium to give α-guanine nucleotide complexes and free βγ subunits. Whether the opening of potassium channels by the recently described G k in isolated membrane patches from mammalian atrial myocytes was mediated by the α k subunit or βγ dimer was tested. The α k subunit was found to be active, while the βγ dimer was inactive in stimulating potassium channel activity. Thus, G k resembles G s , the stimulatory regulatory component of adenylyl cyclase, and transducin, the regulatory component of the visual system, in that it regulates its effector function—the activity of the ligand-gated potassium channel—through its guanine nucleotide binding subunit.

Bibliography

Codina, J., Yatani, A., Grenet, D., Brown, A. M., & Birnbaumer, L. (1987). The Î± Subunit of the GTP Binding Protein G k Opens Atrial Potassium Channels. Science, 236(4800), 442â445.

Authors 5

Juan Codina (first)
Atsuko Yatani (additional)
Dagoberto Grenet (additional)
Arthur M. Brown (additional)
Lutz Birnbaumer (additional)

References 25 Referenced 224

Birnbaumer, L., Nature 327: 21 (1987). (10.1038/327021a0) / Nature (1987)
BROWN, A.M., THE AGONIST EFFECT OF DIHYDROPYRIDINES ON CA-CHANNELS, NATURE 311: 570 (1984). (10.1038/311570a0) / NATURE (1984)
CODINA, J, EFFECTS OF GUANINE-NUCLEOTIDES AND MG ON HUMAN-ERYTHROCYTE NI AND NS, THE REGULATORY COMPONENTS OF ADENYLYL CYCLASE, JOURNAL OF BIOLOGICAL CHEMISTRY 259: 11408 (1984). (10.1016/S0021-9258(18)90876-8) / JOURNAL OF BIOLOGICAL CHEMISTRY (1984)
10.1016/S0021-9258(18)91097-5
CODINA, J, UPDATED PROTOCOLS AND COMMENTS ON THE PURIFICATION WITHOUT USE OF ACTIVATING LIGANDS OF THE COUPLING PROTEIN-NS AND PROTEIN-NI OF THE HORMONE SENSITIVE ADENYLYL CYCLASE, JOURNAL OF RECEPTOR RESEARCH 4: 411 (1984). (10.3109/10799898409042565) / JOURNAL OF RECEPTOR RESEARCH (1984)
Codina, J., Proceedings of the National Academy of Sciences of the United States of America 80: 4276 (1983). / Proceedings of the National Academy of Sciences of the United States of America (1983)
COLQUHOUN, D, ON THE STOCHASTIC PROPERTIES OF BURSTS OF SINGLE ION CHANNEL OPENINGS AND OF CLUSTERS OF BURSTS, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES 300: 1 (1982). / PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES (1982)
10.1007/BF00656997
HESCHELER, J, THE GTP-BINDING PROTEIN, G0, REGULATES NEURONAL CALCIUM CHANNELS, NATURE 325: 445 (1987). (10.1038/325445a0) / NATURE (1987)
HILDEBRANDT, J.D., CHARACTERIZATION BY TWO-DIMENSIONAL PEPTIDE-MAPPING OF THE GAMMA-SUBUNITS OF NS AND NI, THE REGULATORY PROTEINS OF ADENYLYL CYCLASE, AND OF TRANSDUCIN, THE GUANINE NUCLEOTIDE-BINDING PROTEIN OF ROD OUTER SEGMENTS OF THE EYE, JOURNAL OF BIOLOGICAL CHEMISTRY 260: 14867 (1985). (10.1016/S0021-9258(17)38653-2) / JOURNAL OF BIOLOGICAL CHEMISTRY (1985)
KATADA, T, THE INHIBITORY GUANINE NUCLEOTIDE-BINDING REGULATORY COMPONENT OF ADENYLATE-CYCLASE - SUBUNIT DISSOCIATION AND GUANINE NUCLEOTIDE-DEPENDENT HORMONAL INHIBITION, JOURNAL OF BIOLOGICAL CHEMISTRY 259: 3578 (1984). (10.1016/S0021-9258(17)43133-4) / JOURNAL OF BIOLOGICAL CHEMISTRY (1984)
KATADA, T, THE INHIBITORY GUANINE NUCLEOTIDE-BINDING REGULATORY COMPONENT OF ADENYLATE-CYCLASE - SUBUNIT DISSOCIATION AND THE INHIBITION OF ADENYLATE-CYCLASE IN S49 LYMPHOMA CYC- AND WILD-TYPE MEMBRANES, JOURNAL OF BIOLOGICAL CHEMISTRY 259: 3586 (1984). (10.1016/S0021-9258(17)43134-6) / JOURNAL OF BIOLOGICAL CHEMISTRY (1984)
KATADA, T, 2 GUANINE NUCLEOTIDE-BINDING PROTEINS IN RAT-BRAIN SERVING AS THE SPECIFIC SUBSTRATE OF ISLET-ACTIVATING PROTEIN, PERTUSSIS TOXIN - INTERACTION OF THE ALPHA-SUBUNITS WITH BETA-GAMMA-SUBUNITS IN DEVELOPMENT OF THEIR BIOLOGICAL-ACTIVITIES, JOURNAL OF BIOLOGICAL CHEMISTRY 261: 8182 (1986). (10.1016/S0021-9258(19)83894-2) / JOURNAL OF BIOLOGICAL CHEMISTRY (1986)
10.1038/325321a0
10.1038/325321a0
LUX, H.D., PATCH AND WHOLE CELL CALCIUM CURRENTS RECORDED SIMULTANEOUSLY IN SNAIL NEURONS, JOURNAL OF GENERAL PHYSIOLOGY 83: 727 (1984). (10.1085/jgp.83.5.727) / JOURNAL OF GENERAL PHYSIOLOGY (1984)
NEER, E.J., PURIFICATION AND PROPERTIES OF THE INHIBITORY GUANINE-NUCLEOTIDE REGULATORY UNIT OF BRAIN ADENYLATE-CYCLASE, JOURNAL OF BIOLOGICAL CHEMISTRY 259: 14222 (1984). (10.1016/S0021-9258(18)89881-7) / JOURNAL OF BIOLOGICAL CHEMISTRY (1984)
NOMA, A, ATP-REGULATED K+ CHANNELS IN CARDIAC-MUSCLE, NATURE 305: 147 (1983). (10.1038/305147a0) / NATURE (1983)
NORTHUP, J.K., THE SUBUNITS OF THE STIMULATORY REGULATORY COMPONENT OF ADENYLATE-CYCLASE - RESOLUTION OF THE ACTIVATED 45,000-DALTON (ALPHA) SUBUNIT, JOURNAL OF BIOLOGICAL CHEMISTRY 258: 11369 (1983). (10.1016/S0021-9258(17)44427-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1983)
SAKMANN, B, ACETYLCHOLINE ACTIVATION OF SINGLE MUSCARINIC K+ CHANNELS IN ISOLATED PACEMAKER CELLS OF THE MAMMALIAN HEART, NATURE 303: 250 (1983). (10.1038/303250a0) / NATURE (1983)
STERNWEIS, P.C., ISOLATION OF 2 PROTEINS WITH HIGH-AFFINITY FOR GUANINE-NUCLEOTIDES FROM MEMBRANES OF BOVINE BRAIN, JOURNAL OF BIOLOGICAL CHEMISTRY 259: 13806 (1984). (10.1016/S0021-9258(18)89817-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1984)
WILSON, D.L., EFFECT OF LIMITED INTERVAL RESOLUTION ON SINGLE CHANNEL MEASUREMENTS WITH APPLICATION TO CA CHANNELS, IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING 32: 786 (1985). / IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING (1985)
WILSON, D.L., IEEE T PHYSL 83: 727 (1984). / IEEE T PHYSL (1984)
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10.1126/science.2432660

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 4:28 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 9:47 a.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:46 p.m.)
Issued	38 years, 4 months ago (April 24, 1987)
Published	38 years, 4 months ago (April 24, 1987)
Published Print	38 years, 4 months ago (April 24, 1987)

Funders 0

None

BibTeX

@article{Codina_1987, title={The α Subunit of the GTP Binding Protein G k Opens Atrial Potassium Channels}, volume={236}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2436299}, DOI={10.1126/science.2436299}, number={4800}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Codina, Juan and Yatani, Atsuko and Grenet, Dagoberto and Brown, Arthur M. and Birnbaumer, Lutz}, year={1987}, month=apr, pages={442–445} }

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