Single-Stranded DNA Binding Protein Encoded by the virE Locus of Agrobacterium tumefaciens
10.1126/science.240.4851.501
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

The transfer process of T (transfer)-DNA of Agrobacterium tumefaciens is activated after the induction of the expression of the Ti plasmid virulence ( vir ) loci by plant signal molecules such as acetosyringone. The vir gene products then act to generate a free transferable single-stranded copy of the T-DNA, designated the T-strand. Although some vir proteins are responsible for the synthesis of the T-strand, others may mediate T-strand transfer to plant cells as part of a DNA-protein complex. Here, a novel 69 -kilodalton vir -specific single-stranded DNA binding protein is identified in Agrobacterium harboring a nopaline-type Ti plasmid. This protein binds single-stranded but not double-stranded DNA regardless of nucleotide sequence composition. The molecular size of the vir -specific single-stranded DNA binding protein and its relative abundance in acetosyringone-induced Agrobacterium suggested that it might be the product of the virE locus; molecular cloning and expression of the virE region in Escherichia coli confirmed this prediction.

Bibliography

Citovsky, V., De Vos, G., & Zambryski, P. (1988). Single-Stranded DNA Binding Protein Encoded by the virE Locus of Agrobacterium tumefaciens. Science, 240(4851), 501–504.

Authors 3
  1. Vitaly Citovsky (first)
  2. Guido De Vos (additional)
  3. Patricia Zambryski (additional)
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Dates
Type When
Created 18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited 1 year, 7 months ago (Jan. 12, 2024, 11:05 a.m.)
Indexed 3 weeks, 3 days ago (Aug. 5, 2025, 9:09 a.m.)
Issued 37 years, 4 months ago (April 22, 1988)
Published 37 years, 4 months ago (April 22, 1988)
Published Print 37 years, 4 months ago (April 22, 1988)
Funders 0

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@article{Citovsky_1988, title={Single-Stranded DNA Binding Protein Encoded by the virE Locus of Agrobacterium tumefaciens}, volume={240}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.240.4851.501}, DOI={10.1126/science.240.4851.501}, number={4851}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Citovsky, Vitaly and De Vos, Guido and Zambryski, Patricia}, year={1988}, month=apr, pages={501–504} }