DOI: 10.1126/science.2389144. Antibody-Catalyzed Porphyrin Metallation

Antibody-Catalyzed Porphyrin Metallation

10.1126/science.2389144

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

An antibody elicited to a distorted N -methyl porphyrin catalyzed metal ion chelation by the planar porphyrin. At fixed Zn 2+ and Cu 2+ concentrations, the antibody-catalyzed reaction showed saturation kinetics with respect to the substrate mesoporphyrin IX (2) and was inhibited by the hapten, N -methylmesoporphyrin IX (1). The turnover number of 80 hour -1 for antibody-catalyzed metallation of 2 with Zn 2+ compares with an estimated value of 800 hour -1 for ferrochelatase. The antibody also catalyzed the insertion of Co 2+ and Mn 2+ into 2, but it did not catalyze the metallation of protoporphyrin IX (3) or deuteroporphyrin IX (4). The antibody has high affinity for several metalloporphyrins, suggesting an approach to developing antibody-heme catalysts for redox or electron transfer reactions.

Bibliography

Cochran, A. G., & Schultz, P. G. (1990). Antibody-Catalyzed Porphyrin Metallation. Science, 249(4970), 781â783.

Authors 2

Andrea G. Cochran (first)
Peter G. Schultz (additional)

References 22 Referenced 164

10.1016/0003-2697(76)90527-3
DAILEY, H.A., BOVINE FERROCHELATASE - KINETIC-ANALYSIS OF INHIBITION BY N-METHYLPROTOPORPHYRIN, MANGANESE, AND HEME, JOURNAL OF BIOLOGICAL CHEMISTRY 258: 11453 (1983). (10.1016/S0021-9258(17)44247-5) / JOURNAL OF BIOLOGICAL CHEMISTRY (1983)
HONJO, T, ORIGIN OF IMMUNE DIVERSITY - GENETIC-VARIATION AND SELECTION, ANNUAL REVIEW OF BIOCHEMISTRY 54: 803 (1985). (10.1146/annurev.bi.54.070185.004103) / ANNUAL REVIEW OF BIOCHEMISTRY (1985)
HOPF, F.R., PORPHYRINS 2: 161 (1978). / PORPHYRINS (1978)
10.1038/227680a0
LAVALLEE D.K. CHEM BIOCH N SUBSTIT (1988).
LAVALLEE, D.K., MOL STRUCT ENERG 9: 279 (1988). / MOL STRUCT ENERG (1988)
LI, F, BIOMED CHROMATOGR 2: 164 (1987). (10.1002/bmc.1130020408) / BIOMED CHROMATOGR (1987)
PASTERNACK, R.F., SOME VERY RAPID REACTIONS OF PORPHYRINS IN AQUEOUS-SOLUTION, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 98: 1908 (1976). (10.1021/ja00423a043) / JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (1976)
10.1126/science.3787262
ROBERTS, R.G., BIOMED CHROMATOGR 2: 71 (1987). (10.1002/bmc.1130020207) / BIOMED CHROMATOGR (1987)
ROSSI, E, FERROCHELATASE ACTIVITY IN HUMAN-LYMPHOCYTES, AS QUANTIFIED BY A NEW HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD, CLINICAL CHEMISTRY 34: 2481 (1988). (10.1093/clinchem/34.12.2481) / CLINICAL CHEMISTRY (1988)
SCHULTZ, P.G., CATALYTIC ANTIBODIES, ACCOUNTS OF CHEMICAL RESEARCH 22: 287 (1989). (10.1021/ar00164a005) / ACCOUNTS OF CHEMICAL RESEARCH (1989)
SCHULTZ, P.G., CATALYTIC ANTIBODIES, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH 28: 1283 (1989). (10.1002/anie.198912833) / ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH (1989)
SCHULTZ, P.G., ANNU REV IMMUNOL 8: 335 (1990). (10.1146/annurev.iy.08.040190.002003) / ANNU REV IMMUNOL (1990)
10.1021/cen-v068n018.p026
SCHWABACHER, A.W., METALLOSELECTIVE ANTI-PORPHYRIN MONOCLONAL-ANTIBODIES, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 111: 2344 (1989). (10.1021/ja00188a082) / JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (1989)
SEGEL, I.H., ENZYME KINETICS: 59 (1975). / ENZYME KINETICS (1975)
TAKETANI, S, PURIFICATION AND SUBSTRATE-SPECIFICITY OF BOVINE LIVER-FERROCHELATASE, EUROPEAN JOURNAL OF BIOCHEMISTRY 127: 443 (1982). (10.1111/j.1432-1033.1982.tb06892.x) / EUROPEAN JOURNAL OF BIOCHEMISTRY (1982)
TAKETANI, S, RAT-LIVER FERROCHELATASE - PURIFICATION, PROPERTIES, AND STIMULATION BY FATTY-ACIDS, JOURNAL OF BIOLOGICAL CHEMISTRY 256: 2748 (1981). / JOURNAL OF BIOLOGICAL CHEMISTRY (1981)
10.1126/science.3787261
WHITE, W.I., PORPHYRINS 5: 303 (1978). / PORPHYRINS (1978)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:10 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 11:16 a.m.)
Indexed	1 month, 3 weeks ago (July 4, 2025, 7:48 a.m.)
Issued	35 years ago (Aug. 17, 1990)
Published	35 years ago (Aug. 17, 1990)
Published Print	35 years ago (Aug. 17, 1990)

Funders 0

None

BibTeX

@article{Cochran_1990, title={Antibody-Catalyzed Porphyrin Metallation}, volume={249}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2389144}, DOI={10.1126/science.2389144}, number={4970}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Cochran, Andrea G. and Schultz, Peter G.}, year={1990}, month=aug, pages={781–783} }

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