DOI: 10.1126/science.223.4643.1423. X-ray Laue Diffraction from Protein Crystals

X-ray Laue Diffraction from Protein Crystals

10.1126/science.223.4643.1423

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

In conventional x-ray diffraction experiments on single crystals, essentially monochromatic x-rays are used. If polychromatic x-rays derived from a synchrotron radiation spectrum are used, they generate a Laue diffraction pattern. Laue patterns from single crystals of macromolecules can be obtained in less than 1 second, and significant radiation damage does not occur over the course of an exposure. Integrated intensities are obtained without rotation of the crystal, and individual structure factors may be extracted for most reflections. The Laue technique thus offers advantages for the recording of diffraction patterns from short-lived structural intermediates; that is, for time-resolved crystallography.

Bibliography

Moffat, K., Szebenyi, D., & Bilderback, D. (1984). X-ray Laue Diffraction from Protein Crystals. Science, 223(4643), 1423â1425.

Authors 3

Keith Moffat (first)
Doletha Szebenyi (additional)
Donald Bilderback (additional)

References 15 Referenced 135

Amoros J. L. The Laue Method (1975).
ARNDT, U.W., OPTIMIZED ANOMALOUS DISPERSION IN CRYSTALLOGRAPHY - A SYNCHROTRON X-RAY POLYCHROMATIC SIMULTANEOUS PROFILE METHOD, NATURE 298: 835 (1982). (10.1038/298835a0) / NATURE (1982)
BARTUNIK, H.D., LOW-TEMPERATURE AND TIME-RESOLVED PROTEIN CRYSTALLOGRAPHY USING SYNCHROTRON RADIATION, NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH 208: 523 (1983). (10.1016/0167-5087(83)91176-6) / NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH (1983)
10.1126/science.206.4415.157
Bilderback, D. H., Nuclear Instruments and Methods in Physics Research 222: 245 (1984). (10.1016/0167-5087(84)90537-4) / Nuclear Instruments and Methods in Physics Research (1984)
FRIEDRICH, W, P BAVARIAN ACADEMY S: 303 (1912). / P BAVARIAN ACADEMY S (1912)
GREENHOUGH, T.J., OSCILLATION CAMERA DATA-PROCESSING .3. GENERAL DIFFRACTION SPOT SIZE, SHAPE AND ENERGY PROFILE - FORMALISM FOR POLYCHROMATIC DIFFRACTION EXPERIMENTS WITH MONOCHROMATIZED SYNCHROTRON X-RADIATION FROM A SINGLY BENT TRIANGULAR MONOCHROMATOR, JOURNAL OF APPLIED CRYSTALLOGRAPHY 16: 242 (1983). (10.1107/S0021889883010316) / JOURNAL OF APPLIED CRYSTALLOGRAPHY (1983)
GREENHOUGH, T.J., THE USES OF SYNCHROTRON X-RADIATION IN THE CRYSTALLOGRAPHY OF MOLECULAR-BIOLOGY, PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY 41: 67 (1983). (10.1016/0079-6107(83)90026-3) / PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY (1983)
HUBBARD, C.R., HIGHLY EFFICIENT NEUTRON-DIFFRACTION TECHNIQUE - USE OF WHITE RADIATION, ACTA CRYSTALLOGRAPHICA SECTION A-CRYSTAL PHYSICS DIFFRACTION THEORETICAL AND GENERAL CRYSTALLOGRAPHY 28: 236 (1972). (10.1107/S0567739472000658) / ACTA CRYSTALLOGRAPHICA SECTION A-CRYSTAL PHYSICS DIFFRACTION THEORETICAL AND GENERAL CRYSTALLOGRAPHY (1972)
LOWDE, R.D., A NEW RATIONALE OF STRUCTURE-FACTOR MEASUREMENT IN NEUTRON-DIFFRACTION ANALYSIS, ACTA CRYSTALLOGRAPHICA 9: 151 (1956). (10.1107/S0365110X56000346) / ACTA CRYSTALLOGRAPHICA (1956)
NUNES, A.C., NEUTRON FOURIER CHOPPER IN PROTEIN CRYSTALLOGRAPHY, JOURNAL OF APPLIED CRYSTALLOGRAPHY 8: 20 (1975). (10.1107/S0021889875009466) / JOURNAL OF APPLIED CRYSTALLOGRAPHY (1975)
PARKHURST, L.J., REACTION OF CARBON MONOXIDE WITH HORSE HEMOGLOBIN IN SOLUTION IN ERYTHROCYTES AND IN CRYSTALS, JOURNAL OF BIOLOGICAL CHEMISTRY 242: 5762 (1967). (10.1016/S0021-9258(18)99366-X) / JOURNAL OF BIOLOGICAL CHEMISTRY (1967)
Stuhrmann, H. B., Synchrotron Radiation Research: 513 (1980). / Synchrotron Radiation Research (1980)
WOOD, I.G., A CRYSTAL-STRUCTURE REFINEMENT FROM LAUE PHOTOGRAPHS TAKEN WITH SYNCHROTRON RADIATION, ACTA CRYSTALLOGRAPHICA SECTION B-STRUCTURAL SCIENCE 39: 543 (1983). (10.1107/S0108768183002931) / ACTA CRYSTALLOGRAPHICA SECTION B-STRUCTURAL SCIENCE (1983)
Zachariasen W. H. Theory of X-Ray Diffraction in Crystals (1945).

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 3:41 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 3:27 a.m.)
Indexed	3 weeks, 1 day ago (Aug. 5, 2025, 8:56 a.m.)
Issued	41 years, 4 months ago (March 30, 1984)
Published	41 years, 4 months ago (March 30, 1984)
Published Print	41 years, 4 months ago (March 30, 1984)

Funders 0

None

BibTeX

@article{Moffat_1984, title={X-ray Laue Diffraction from Protein Crystals}, volume={223}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.223.4643.1423}, DOI={10.1126/science.223.4643.1423}, number={4643}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Moffat, Keith and Szebenyi, Doletha and Bilderback, Donald}, year={1984}, month=mar, pages={1423–1425} }

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