DOI: 10.1126/science.221977. Phospholipid Methylation Unmasks Cryptic β-Adrenergic Receptors in Rat Reticulocytes

Phospholipid Methylation Unmasks Cryptic β-Adrenergic Receptors in Rat Reticulocytes

10.1126/science.221977

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The effect of phospholipid methylation on the number of β-adrenergic receptor binding sites was examined in rat reticulocyte membranes. Stimulation of phosphatidylcholine synthesis by the introduction of the methyl donor S -adenosyl-L-methionine into reticulocyte ghosts increased the number of β-adrenergic receptor binding sites. The appearance of β-adrenergic binding sites was dependent on the formation of phosphatidylcholine by the enzyme that converts phosphatidyl- N -monomethylethanolamine to phosphatidylcholine, but not on the synthesis of phosphatidyl- N -monomethylethanolamine from phosphatidylethanolamine. Both the synthesis of phosphatidylcholine and the unmasking of cryptic receptors were time and temperature dependent and did not occur in the presence of the methyl transferase inhibitor, S -adenosyl-L-homocysteine.

Bibliography

Strittmatter, W. J., Hirata, F., & Axelrod, J. (1979). Phospholipid Methylation Unmasks Cryptic Î²-Adrenergic Receptors in Rat Reticulocytes. Science, 204(4398), 1205â1207.

Authors 3

Warren J. Strittmatter (first)
Fusao Hirata (additional)
Julius Axelrod (additional)

References 14 Referenced 146

BILEZIKIAN, J.P., IDENTIFICATION AND PERSISTENCE OF BETA-ADRENERGIC RECEPTORS DURING MATURATION OF RAT RETICULOCYTE, MOLECULAR PHARMACOLOGY 13: 775 (1977). / MOLECULAR PHARMACOLOGY (1977)
HIRATA, F, ENZYMATIC METHYLATION OF PHOSPHATIDYLETHANOLAMINE INCREASES ERYTHROCYTE-MEMBRANE FLUIDITY, NATURE 275: 219 (1978). (10.1038/275219a0) / NATURE (1978)
HIRATA, F, IDENTIFICATION AND PROPERTIES OF 2 METHYLTRANSFERASES IN CONVERSION OF PHOSPHATIDYLETHANOLAMINE TO PHOSPHATIDYLCHOLINE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 75: 1718 (1978). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1978)
HIRATA, F, ENZYMATIC-SYNTHESIS AND RAPID TRANSLOCATION OF PHOSPHATIDYLCHOLINE BY 2 METHYLTRANSFERASES IN ERYTHROCYTE-MEMBRANES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 75: 2348 (1978). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1978)
HIRATA, F, BETA-ADRENERGIC-RECEPTOR AGONISTS INCREASE PHOSPHOLIPID METHYLATION, MEMBRANE FLUIDITY, AND BETA-ADRENERGIC RECEPTOR-ADENYLATE CYCLASE COUPLING, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 76: 368 (1979). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1979)
HOKIN, L.E., RECEPTORS HORMONE AC: 447 (1977). / RECEPTORS HORMONE AC (1977)
JACKSON, R.L., RECEPTORS HORMONE AC: 411 (1977). / RECEPTORS HORMONE AC (1977)
LEFKOWITZ, R.J., RECENT PROGRESS IN HORMONE RESEARCH 32: 597 (1976). / RECENT PROGRESS IN HORMONE RESEARCH (1976)
LYLES, D.S., SENDAI VIRUS-INDUCED HEMOLYSIS - REDUCTION IN HETEROGENEITY OF ERYTHROCYTE LIPID BILAYER FLUIDITY, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 74: 1918 (1977). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1977)
MUKHERJEE, C, REGULATION OF ADENYLATE-CYCLASE COUPLED BETA-ADRENERGIC RECEPTORS BY BETA-ADRENERGIC CATECHOLAMINES, ENDOCRINOLOGY 99: 347 (1976). (10.1210/endo-99-2-347) / ENDOCRINOLOGY (1976)
MUKHERJEE, C, DESENSITIZATION OF BETA-ADRENERGIC RECEPTORS BY BETA-ADRENERGIC AGONISTS IN A CELL-FREE SYSTEM - RESENSITIZATION BY GUANOSINE 5'-(BETA,GAMMA-IMINO) TRIPHOSPHATE AND OTHER PURINE NUCLEOTIDES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 73: 1494 (1976). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1976)
ROMERO, J.A., CIRCADIAN CYCLES IN BINDING OF ALPRENOLOL-H-3 TO BETA-ADRENERGIC-RECEPTOR SITES IN RAT PINEAL, NATURE 258: 435 (1975). (10.1038/258435a0) / NATURE (1975)
ROMERO, J.A., PRE POSTSYNAPTIC REC: 265 (1975). / PRE POSTSYNAPTIC REC (1975)
SHAMOO, A.E., ISOLATION OF IONOPHORES FROM ION-TRANSPORT SYSTEMS AND THEIR ROLE IN ENERGY TRANSDUCTION, BIOCHIMICA ET BIOPHYSICA ACTA 472: 13 (1977). (10.1016/0304-4157(77)90013-2) / BIOCHIMICA ET BIOPHYSICA ACTA (1977)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 12:39 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 3:26 a.m.)
Indexed	1 year, 3 months ago (May 12, 2024, 3:56 p.m.)
Issued	46 years, 2 months ago (June 15, 1979)
Published	46 years, 2 months ago (June 15, 1979)
Published Print	46 years, 2 months ago (June 15, 1979)

Funders 0

None

BibTeX

@article{Strittmatter_1979, title={Phospholipid Methylation Unmasks Cryptic β-Adrenergic Receptors in Rat Reticulocytes}, volume={204}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.221977}, DOI={10.1126/science.221977}, number={4398}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Strittmatter, Warren J. and Hirata, Fusao and Axelrod, Julius}, year={1979}, month=jun, pages={1205–1207} }

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