DOI: 10.1126/science.2157284. Binding of GAP to Activated PDGF Receptors

Binding of GAP to Activated PDGF Receptors

10.1126/science.2157284

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The ras proto-oncogene products appear to relay intracellular signals via the Ras guanosine triphosphatase (GTPase) activator protein, GAP. In dog epithelial cells expressing human platelet-derived growth factor (PDGF) receptors, binding of PDGF caused approximately one-tenth of the total GAP molecules to complex with the receptor. Studies with mutant PDGF receptors showed that maximum association required both receptor kinase activity and phosphorylatable tyrosine residues at both the identified sites of receptor autophosphorylation.

Bibliography

Kazlauskas, A., Ellis, C., Pawson, T., & Cooper, J. A. (1990). Binding of GAP to Activated PDGF Receptors. Science, 247(4950), 1578â1581.

Authors 4

Andrius Kazlauskas (first)
Christine Ellis (additional)
Tony Pawson (additional)
Jonathan A. Cooper (additional)

References 32 Referenced 262

10.1126/science.2833817
BALLESTER, R, GENETIC-ANALYSIS OF MAMMALIAN GAP EXPRESSED IN YEAST, CELL 59: 681 (1989). (10.1016/0092-8674(89)90014-7) / CELL (1989)
10.1146/annurev.bi.56.070187.004023
CALES, C, THE CYTOPLASMIC PROTEIN GAP IS IMPLICATED AS THE TARGET FOR REGULATION BY THE RAS GENE-PRODUCT, NATURE 332: 548 (1988). (10.1038/332548a0) / NATURE (1988)
CLAESSONWELSH, L, CDNA CLONING AND EXPRESSION OF A HUMAN PLATELET-DERIVED GROWTH-FACTOR (PDGF) RECEPTOR SPECIFIC FOR B-CHAIN-CONTAINING PDGF MOLECULES, MOLECULAR AND CELLULAR BIOLOGY 8: 3476 (1988). / MOLECULAR AND CELLULAR BIOLOGY (1988)
10.1126/science.2466336
10.1038/343377a0
FRACKELTON, A.R., EVIDENCE FOR THE PLATELET-DERIVED GROWTH FACTOR-STIMULATED TYROSINE PHOSPHORYLATION OF THE PLATELET-DERIVED GROWTH-FACTOR RECEPTOR INVIVO - IMMUNOPURIFICATION USING A MONOCLONAL-ANTIBODY TO PHOSPHOTYROSINE, JOURNAL OF BIOLOGICAL CHEMISTRY 259: 7909 (1984). (10.1016/S0021-9258(17)42879-1) / JOURNAL OF BIOLOGICAL CHEMISTRY (1984)
FREISSMUTH, M, G-PROTEINS CONTROL DIVERSE PATHWAYS OF TRANSMEMBRANE SIGNALING, FASEB JOURNAL 3: 2125 (1989). (10.1096/fasebj.3.10.2546847) / FASEB JOURNAL (1989)
10.1073/pnas.85.14.5026
GIBBS, J.B., XENOPUS OOCYTE GERMINAL-VESICLE BREAKDOWN INDUCED BY [VAL12]RAS IS INHIBITED BY A CYTOSOL-LOCALIZED RAS MUTANT, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86: 6630 (1989). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1989)
GRONWALD, RGK, CLONING AND EXPRESSION OF A CDNA CODING FOR THE HUMAN PLATELET-DERIVED GROWTH-FACTOR RECEPTOR - EVIDENCE FOR MORE THAN ONE RECEPTOR CLASS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 3435 (1988). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1988)
HART, C.E., SYNTHESIS, PHOSPHORYLATION, AND DEGRADATION OF MULTIPLE FORMS OF THE PLATELET-DERIVED GROWTH-FACTOR RECEPTOR STUDIED USING A MONOCLONAL-ANTIBODY, JOURNAL OF BIOLOGICAL CHEMISTRY 262: 10780 (1987). (10.1016/S0021-9258(18)61031-2) / JOURNAL OF BIOLOGICAL CHEMISTRY (1987)
HUHN, R.D., IDENTIFICATION OF TYROSINE-PHOSPHORYLATED COLONY-STIMULATING FACTOR-I (CSF-1) RECEPTOR AND A 56-KILODALTON PROTEIN PHOSPHORYLATED IN INTACT HUMAN-CELLS IN RESPONSE TO CSF-1, JOURNAL OF CELLULAR BIOCHEMISTRY 39: 129 (1989). (10.1002/jcb.240390205) / JOURNAL OF CELLULAR BIOCHEMISTRY (1989)
KAZLAUSKAS, A, AUTOPHOSPHORYLATION OF THE PDGF RECEPTOR IN THE KINASE INSERT REGION REGULATES INTERACTIONS WITH CELL-PROTEINS, CELL 58: 1121 (1989). (10.1016/0092-8674(89)90510-2) / CELL (1989)
KAZLAUSKAS, A, PROTEIN KINASE-C MEDIATES PLATELET-DERIVED GROWTH-FACTOR INDUCED TYROSINE PHOSPHORYLATION OF P42, JOURNAL OF CELL BIOLOGY 106: 1395 (1988). (10.1083/jcb.106.4.1395) / JOURNAL OF CELL BIOLOGY (1988)
KAZLAUSKAS A unpublished data.
10.1016/0092-8674(89)90985-9
KUMJIAN, D.A., PLATELET-DERIVED GROWTH-FACTOR (PDGF) BINDING PROMOTES PHYSICAL ASSOCIATION OF PDGF RECEPTOR WITH PHOSPHOLIPASE-C, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86: 8232 (1989). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1989)
10.1126/science.2536956
10.1016/0092-8674(89)90976-8
10.1038/342711a0
MORRISON, D.K., DIRECT ACTIVATION OF THE SERINE THREONINE KINASE-ACTIVITY OF RAF-1 THROUGH TYROSINE PHOSPHORYLATION BY THE PDGF BETA-RECEPTOR, CELL 58: 649 (1989). (10.1016/0092-8674(89)90100-1) / CELL (1989)
MULCAHY, L.S., REQUIREMENT FOR RAS PROTO-ONCOGENE FUNCTION DURING SERUM-STIMULATED GROWTH OF NIH-3T3-CELLS, NATURE 313: 241 (1985). (10.1038/313241a0) / NATURE (1985)
SANTOS, E, STRUCTURAL AND FUNCTIONAL-PROPERTIES OF RAS PROTEINS, FASEB JOURNAL 3: 2151 (1989). (10.1096/fasebj.3.10.2666231) / FASEB JOURNAL (1989)
TANAKA, K, IRA1, AN INHIBITORY REGULATOR OF THE RAS-CYCLIC AMP PATHWAY IN SACCHAROMYCES-CEREVISIAE, MOLECULAR AND CELLULAR BIOLOGY 9: 757 (1989). / MOLECULAR AND CELLULAR BIOLOGY (1989)
10.1126/science.2821624
10.1126/science.3201259
TURKER, M.S., A NOVEL CLASS OF UNSTABLE 6-THIOGUANINE-RESISTANT CELLS FROM DOG AND HUMAN KIDNEYS, CELL BIOLOGY AND TOXICOLOGY 4: 211 (1988). (10.1007/BF00119247) / CELL BIOLOGY AND TOXICOLOGY (1988)
10.1038/335090a0
WHITMAN, M, TYPE-I PHOSPHATIDYLINOSITOL KINASE MAKES A NOVEL INOSITOL PHOSPHOLIPID, PHOSPHATIDYLINOSITOL-3-PHOSPHATE, NATURE 332: 644 (1988). (10.1038/332644a0) / NATURE (1988)
10.1038/323226a0

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:10 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 5:25 a.m.)
Indexed	3 weeks, 1 day ago (Aug. 2, 2025, 12:09 a.m.)
Issued	35 years, 4 months ago (March 30, 1990)
Published	35 years, 4 months ago (March 30, 1990)
Published Print	35 years, 4 months ago (March 30, 1990)

Funders 0

None

BibTeX

@article{Kazlauskas_1990, title={Binding of GAP to Activated PDGF Receptors}, volume={247}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2157284}, DOI={10.1126/science.2157284}, number={4950}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Kazlauskas, Andrius and Ellis, Christine and Pawson, Tony and Cooper, Jonathan A.}, year={1990}, month=mar, pages={1578–1581} }

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