DOI: 10.1126/science.2154036. Growth Factors Induce Phosphorylation of the Na <sup>+</sup> /H <sup>+</sup> Antiporter, a Glycoprotein of 110 kD

Growth Factors Induce Phosphorylation of the Na + /H + Antiporter, a Glycoprotein of 110 kD

10.1126/science.2154036

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The Na + /H + antiporter, which regulates intracellular p H in virtually all cells, is one of the best examples of a mitogen- and oncogene-activated membrane target whose activity rapidly changes on stimulation. The activating mechanism is unknown. A Na + /H + antiporter complementary DNA fragment was expressed in Escherichia coli as a β-galactosidase fusion protein, and a specific antibody to the fusion protein was prepared. Use of this antibody revealed that the Na + /H + antiporter is a 110-kilodalton glycoprotein that is phosphorylated in growing cells. Mitogenic activation of resting hamster fibroblasts and A431 human epidermoid cells with epidermal growth factor, thrombin, phorbol esters, or serum, stimulated phosphorylation of the Na + /H + antiporter with a time course similar to that of the rise in intracellular p H.

Bibliography

Sardet, C., Counillon, L., Franchi, A., & PouyssÃ©gur, J. (1990). Growth Factors Induce Phosphorylation of the Na + /H + Antiporter, a Glycoprotein of 110 kD. Science, 247(4943), 723â726.

Authors 4

C. Sardet (first)
L. Counillon (additional)
A. Franchi (additional)
J. Pouysségur (additional)

References 28 Referenced 336

ARONSON P NA PLUS H PLUS EXCHA (1986).
10.1038/326800a0
COOPER, J.A., DETECTION AND QUANTIFICATION OF PHOSPHOTYROSINE IN PROTEINS, METHODS IN ENZYMOLOGY 99: 387 (1983). (10.1016/0076-6879(83)99075-4) / METHODS IN ENZYMOLOGY (1983)
FRANCHI, A, ISOLATION AND PROPERTIES OF FIBROBLAST MUTANTS OVEREXPRESSING AN ALTERED NA+/H+ ANTIPORTER, JOURNAL OF BIOLOGICAL CHEMISTRY 261: 4614 (1986). / JOURNAL OF BIOLOGICAL CHEMISTRY (1986)
FRANCHI A unpublished data.
GANZ, M.B., ARGININE VASOPRESSIN ENHANCES PHI REGULATION IN THE PRESENCE OF HCO3- BY STIMULATING 3 ACID-BASE TRANSPORT-SYSTEMS, NATURE 337: 648 (1989). (10.1038/337648a0) / NATURE (1989)
GRINSTEIN S NA PLUS H PLUS EXCHA (1988).
KOZMA, S.C., UNMASKING A GROWTH-FACTOR ONCOGENE-ACTIVATED S6 PHOSPHORYLATION CASCADE, CELLULAR SIGNALLING 1: 219 (1989). (10.1016/0898-6568(89)90039-9) / CELLULAR SIGNALLING (1989)
LALLEMAIN, G, EGF AND INSULIN ACTION IN FIBROBLASTS - EVIDENCE THAT PHOSPHOINOSITIDE HYDROLYSIS IS NOT AN ESSENTIAL MITOGENIC SIGNALING PATHWAY, FEBS LETTERS 197: 344 (1986). (10.1016/0014-5793(86)80354-4) / FEBS LETTERS (1986)
LALLEMAIN, G, GROWTH FACTORS 1: 311 (1989). (10.3109/08977198909000255) / GROWTH FACTORS (1989)
LALLEMAIN, G, GROWTH-FACTOR ACTION AND INTRACELLULAR PH REGULATION IN FIBROBLASTS - EVIDENCE FOR A MAJOR ROLE OF THE NA+/H+ ANTIPORT, JOURNAL OF BIOLOGICAL CHEMISTRY 259: 5809 (1984). (10.1016/S0021-9258(18)91086-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1984)
LALLEMAIN, G, ROLE OF A NA+-DEPENDENT CL-HCO3- EXCHANGE IN REGULATION OF INTRACELLULAR PH IN FIBROBLASTS, JOURNAL OF BIOLOGICAL CHEMISTRY 260: 4877 (1985). (10.1016/S0021-9258(18)89153-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1985)
MOOLENAAR, W.H., EFFECTS OF GROWTH-FACTORS ON INTRACELLULAR PH REGULATION, ANNUAL REVIEW OF PHYSIOLOGY 48: 363 (1986). (10.1146/annurev.ph.48.030186.002051) / ANNUAL REVIEW OF PHYSIOLOGY (1986)
MOOLENAAR, W.H., NA+/H+ EXCHANGE AND CYTOPLASMIC PH IN THE ACTION OF GROWTH-FACTORS IN HUMAN-FIBROBLASTS, NATURE 304: 645 (1983). (10.1038/304645a0) / NATURE (1983)
MORRISON, D.K., SIGNAL TRANSDUCTION FROM MEMBRANE TO CYTOPLASM - GROWTH-FACTORS AND MEMBRANE-BOUND ONCOGENE PRODUCTS INCREASE RAF-1 PHOSPHORYLATION AND ASSOCIATED PROTEIN-KINASE ACTIVITY, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 8855 (1988). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1988)
PARIS, S, GROWTH-FACTORS ACTIVATE THE NA+/H+ ANTIPORTER IN QUIESCENT FIBROBLASTS BY INCREASING ITS AFFINITY FOR INTRACELLULAR H+, JOURNAL OF BIOLOGICAL CHEMISTRY 259: 10989 (1984). (10.1016/S0021-9258(18)90611-3) / JOURNAL OF BIOLOGICAL CHEMISTRY (1984)
POUYSSEGUR, J, GENETIC APPROACH TO STRUCTURE, FUNCTION, AND REGULATION OF THE NA+/H+ ANTIPORTER, KIDNEY INTERNATIONAL 32: S144 (1987). / KIDNEY INTERNATIONAL (1987)
POUYSSEGUR, J, A SPECIFIC MUTATION ABOLISHING NA+/H+ ANTIPORT ACTIVITY IN HAMSTER FIBROBLASTS PRECLUDES GROWTH AT NEUTRAL AND ACIDIC PH, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 81: 4833 (1984). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1984)
POUYSSEGUR, J, THE GROWTH FACTOR-ACTIVATABLE NA+/H+ EXCHANGE SYSTEM - A GENETIC APPROACH, TRENDS IN BIOCHEMICAL SCIENCES 10: 453 (1985). (10.1016/0968-0004(85)90030-1) / TRENDS IN BIOCHEMICAL SCIENCES (1985)
ROSSOMANDO, A.J., EVIDENCE THAT PP42, A MAJOR TYROSINE KINASE TARGET PROTEIN, IS A MITOGEN-ACTIVATED SERINE THREONINE PROTEIN-KINASE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86: 6940 (1989). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1989)
10.1126/science.3018928
SARDET, C, MOLECULAR-CLONING, PRIMARY STRUCTURE, AND EXPRESSION OF THE HUMAN GROWTH FACTOR-ACTIVATABLE NA+/H+ ANTIPORTER, CELL 56: 271 (1989). (10.1016/0092-8674(89)90901-X) / CELL (1989)
SARDET, C, MOLECULAR-CLONING OF THE GROWTH-FACTOR-ACTIVATABLE HUMAN NA+/H+ ANTIPORTER, COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY 53: 1011 (1988). (10.1101/SQB.1988.053.01.116) / COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY (1988)
SARDET C unpublished data.
10.1016/0092-8674(88)90051-7
TSE C.M. unpublished data.
VANOBBERGHENSCH.E, ALPHA-THROMBIN-INDUCED EARLY MITOGENIC SIGNALING EVENTS AND G0 TO S-PHASE TRANSITION OF FIBROBLASTS REQUIRE CONTINUAL EXTERNAL STIMULATION, EMBO JOURNAL 4: 2927 (1985). (10.1002/j.1460-2075.1985.tb04025.x) / EMBO JOURNAL (1985)
YUSUFI, ANK, ROLE OF N-LINKED OLIGOSACCHARIDES IN THE TRANSPORT ACTIVITY OF THE NA+/H+ ANTIPORTER IN RAT RENAL BRUSH-BORDER MEMBRANE, JOURNAL OF BIOLOGICAL CHEMISTRY 263: 13683 (1988). (10.1016/S0021-9258(18)68295-X) / JOURNAL OF BIOLOGICAL CHEMISTRY (1988)

Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 6:10 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 5:12 a.m.)
Indexed	3 days, 1 hour ago (Sept. 4, 2025, 10:21 a.m.)
Issued	35 years, 6 months ago (Feb. 9, 1990)
Published	35 years, 6 months ago (Feb. 9, 1990)
Published Print	35 years, 6 months ago (Feb. 9, 1990)

Funders 0

None

BibTeX

@article{Sardet_1990, title={Growth Factors Induce Phosphorylation of the Na + /H + Antiporter, a Glycoprotein of 110 kD}, volume={247}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2154036}, DOI={10.1126/science.2154036}, number={4943}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Sardet, C. and Counillon, L. and Franchi, A. and Pouysségur, J.}, year={1990}, month=feb, pages={723–726} }

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        2,
        9
      ]
    ]
  },
  "references-count": 28,
  "journal-issue": {
    "issue": "4943",
    "published-print": {
      "date-parts": [
        [
          1990,
          2,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.2154036"
  ],
  "URL": "http://dx.doi.org/10.1126/science.2154036",
  "relation": {},
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1990,
        2,
        9
      ]
    ]
  }
}