DOI: 10.1126/science.2143033. Random Peptide Libraries: a Source of Specific Protein Binding Molecules

Random Peptide Libraries: a Source of Specific Protein Binding Molecules

10.1126/science.2143033

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Libraries of random peptide sequences were constructed and screened to identify peptides that specifically bind to proteins. In one of these about 2 × 10 7 different 15-residue peptide sequences were expressed on the surface of the coliphage M13. Each phage encoded a single random sequence and expressed it as a fusion complex with pIII, a minor coat protein present at five molecules per phage. Phage encoding nine different streptavidin-binding peptide sequences were isolated from this library. The core consensus sequence was His-Pro-Gln and binding of these phage to streptavidin was inhibited by biotin. This type of library makes it possible to identify peptides that bind to proteins (or other macromolecules) that have no previously known affinity for peptides.

Bibliography

Devlin, J. J., Panganiban, L. C., & Devlin, P. E. (1990). Random Peptide Libraries: a Source of Specific Protein Binding Molecules. Science, 249(4967), 404â406.

Authors 3

James J. Devlin (first)
Lucy C. Panganiban (additional)
Patricia E. Devlin (additional)

References 12 Referenced 736

DOWER, W.J., HIGH-EFFICIENCY TRANSFORMATION OF ESCHERICHIA-COLI BY HIGH-VOLTAGE ELECTROPORATION, NUCLEIC ACIDS RESEARCH 16: 6127 (1988). (10.1093/nar/16.13.6127) / NUCLEIC ACIDS RESEARCH (1988)
GRAY, C.W., ADSORPTION COMPLEX OF FILAMENTOUS FD VIRUS, JOURNAL OF MOLECULAR BIOLOGY 146: 621 (1981). (10.1016/0022-2836(81)90050-4) / JOURNAL OF MOLECULAR BIOLOGY (1981)
INNIS M.A. PCR PROTOCOLS GUIDE (1990).
KATCHALSKI, E, ASPECTS PROTEIN STRU: 205 (1963). / ASPECTS PROTEIN STRU (1963)
LIN, T.C., ISOLATION AND CHARACTERIZATION OF THE C-PROTEIN AND D-PROTEIN CODED BY GENE-IX AND GENE-VI IN THE FILAMENTOUS BACTERIOPHAGE-FL AND BACTERIOPHAGE-FD, JOURNAL OF BIOLOGICAL CHEMISTRY 255: 10331 (1980). (10.1016/S0021-9258(19)70468-2) / JOURNAL OF BIOLOGICAL CHEMISTRY (1980)
PARMLEY, S.F., ANTIBODY-SELECTABLE FILAMENTOUS FD PHAGE VECTORS - AFFINITY PURIFICATION OF TARGET GENES, GENE 73: 305 (1988). (10.1016/0378-1119(88)90495-7) / GENE (1988)
10.1126/science.3388019
Sambrook J. Molecular Cloning: A Laboratory Manual (1989).
10.1126/science.1696028
10.1126/science.4001944
10.1093/nar/14.11.4683
ZINDER, N.D., THE FILAMENTOUS PHAGE (FF) AS VECTORS FOR RECOMBINANT DNA - A REVIEW, GENE 19: 1 (1982). (10.1016/0378-1119(82)90183-4) / GENE (1982)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:10 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 5:23 a.m.)
Indexed	2 weeks, 6 days ago (Aug. 5, 2025, 8:49 a.m.)
Issued	35 years ago (July 27, 1990)
Published	35 years ago (July 27, 1990)
Published Print	35 years ago (July 27, 1990)

Funders 0

None

BibTeX

@article{Devlin_1990, title={Random Peptide Libraries: a Source of Specific Protein Binding Molecules}, volume={249}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2143033}, DOI={10.1126/science.2143033}, number={4967}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Devlin, James J. and Panganiban, Lucy C. and Devlin, Patricia E.}, year={1990}, month=jul, pages={404–406} }

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