DOI: 10.1126/science.2138816. T Cell Antigen Receptor-Mediated Activation of Phospholipase C Requires Tyrosine Phosphorylation

T Cell Antigen Receptor-Mediated Activation of Phospholipase C Requires Tyrosine Phosphorylation

10.1126/science.2138816

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Triggering of the antigen-specific T cell receptor-CD3 complex (TCR-CD3) stimulates a rapid phospholipase C-mediated hydrolysis of inositol phospholipids, resulting in the production of second messengers and in T cell activation and proliferation. The role of tyrosine phosphorylation in these events was investigated with a tyrosine protein kinase (TPK) inhibitor, genistein. At doses that inhibited TPK activity and tyrosine phosphorylation of the TCR ζ subunit, but not phospholipase C activity, genistein prevented TCR-CD3-mediated phospholipase C activation, interleukin-2 receptor expression, and T cell proliferation. These findings indicate that tyrosine phosphorylation is an early and critical event that most likely precedes, and is a prerequisite for, inositol phospholipid breakdown during receptor-mediated T cell activation.

Bibliography

Mustelin, T., Coggeshall, K. M., Isakov, N., & Altman, A. (1990). T Cell Antigen Receptor-Mediated Activation of Phospholipase C Requires Tyrosine Phosphorylation. Science, 247(4950), 1584â1587.

Authors 4

T. Mustelin (first)
K. M. Coggeshall (additional)
N. Isakov (additional)
A. Altman (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:10 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 5:25 a.m.)
Indexed	3 months ago (May 20, 2025, 9:31 a.m.)
Issued	35 years, 4 months ago (March 30, 1990)
Published	35 years, 4 months ago (March 30, 1990)
Published Print	35 years, 4 months ago (March 30, 1990)

Funders 0

None

BibTeX

@article{Mustelin_1990, title={T Cell Antigen Receptor-Mediated Activation of Phospholipase C Requires Tyrosine Phosphorylation}, volume={247}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2138816}, DOI={10.1126/science.2138816}, number={4950}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Mustelin, T. and Coggeshall, K. M. and Isakov, N. and Altman, A.}, year={1990}, month=mar, pages={1584–1587} }

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