DOI: 10.1126/science.2119055. Site-Specific Gene Expression in Vivo by Direct Gene Transfer into the Arterial Wall

Site-Specific Gene Expression in Vivo by Direct Gene Transfer into the Arterial Wall

10.1126/science.2119055

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A recombinant β-galactosidase gene has been expressed in a specific arterial segment in vivo by direct infection with a murine amphotropic retroviral vector or by DNA transfection with the use of liposomes. Several cell types in the vessel wall were transduced, including endothelial and vascular smooth muscle cells. After retroviral infection, a recombinant reporter gene was expressed for at least 5 months, and no helper virus was detected. Recombinant gene expression achieved by direct retroviral infection or liposome-mediated DNA transfection was limited to the site of infection and was absent from liver, lung, kidney, and spleen. These results demonstrate that site-specific gene expression can be achieved by direct gene transfer in vivo and could be applied to the treatment of such human diseases as atherosclerosis or cancer.

Bibliography

Nabel, E. G., Plautz, G., & Nabel, G. J. (1990). Site-Specific Gene Expression in Vivo by Direct Gene Transfer into the Arterial Wall. Science, 249(4974), 1285â1288.

Authors 3

Elizabeth G. Nabel (first)
Gregory Plautz (additional)
Gary J. Nabel (additional)

References 22 Referenced 428

Cone, R. D., Proceedings of the National Academy of Sciences of the United States of America 81: 6349 (1984). / Proceedings of the National Academy of Sciences of the United States of America (1984)
Dannenberg, A. M., Methods for Studying Mononuclear Phagocytes: 375 (1981). / Methods for Studying Mononuclear Phagocytes (1981)
DANOS, O, SAFE AND EFFICIENT GENERATION OF RECOMBINANT RETROVIRUSES WITH AMPHOTROPIC AND ECOTROPIC HOST RANGES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 6460 (1988). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1988)
DICHEK, D.A., SEEDING OF INTRAVASCULAR STENTS WITH GENETICALLY ENGINEERED ENDOTHELIAL-CELLS, CIRCULATION 80: 1347 (1989). (10.1161/01.CIR.80.5.1347) / CIRCULATION (1989)
FAXON, D.P., RESTENOSIS FOLLOWING TRANS-LUMINAL ANGIOPLASTY IN EXPERIMENTAL ATHEROSCLEROSIS, ARTERIOSCLEROSIS 4: 189 (1984). (10.1161/01.ATV.4.3.189) / ARTERIOSCLEROSIS (1984)
10.1016/0092-8674(90)90591-2
KODAMA, T, TYPE-I MACROPHAGE SCAVENGER RECEPTOR CONTAINS ALPHA-HELICAL AND COLLAGEN-LIKE COILED COILS, NATURE 343: 531 (1990). (10.1038/343531a0) / NATURE (1990)
10.1016/0092-8674(83)90344-6
Mayer, R. J., Immunochemical Methods in Cell and Molecular Biology: 263 (1987). / Immunochemical Methods in Cell and Molecular Biology (1987)
MCBRIDE, W, RESTENOSIS AFTER SUCCESSFUL CORONARY ANGIOPLASTY - PATHO-PHYSIOLOGY AND PREVENTION, NEW ENGLAND JOURNAL OF MEDICINE 318: 1734 (1988). (10.1056/NEJM198806303182606) / NEW ENGLAND JOURNAL OF MEDICINE (1988)
10.1126/science.2499928
NABEL E.G. unpublished data.
10.1073/pnas.84.1.156
10.1016/0021-9150(82)90104-6
ROHRER, L, COILED-COIL FIBROUS DOMAINS MEDIATE LIGAND-BINDING BY MACROPHAGE SCAVENGER RECEPTOR TYPE-II, NATURE 343: 570 (1990). (10.1038/343570a0) / NATURE (1990)
SCHWARTZ, L, ASPIRIN AND DIPYRIDAMOLE IN THE PREVENTION OF RESTENOSIS AFTER PERCUTANEOUS TRANS-LUMINAL CORONARY ANGIOPLASTY, NEW ENGLAND JOURNAL OF MEDICINE 318: 1714 (1988). (10.1056/NEJM198806303182603) / NEW ENGLAND JOURNAL OF MEDICINE (1988)
STEELE, P.M., BALLOON ANGIOPLASTY - NATURAL-HISTORY OF THE PATHOPHYSIOLOGICAL RESPONSE TO INJURY IN A PIG MODEL, CIRCULATION RESEARCH 57: 105 (1985). (10.1161/01.RES.57.1.105) / CIRCULATION RESEARCH (1985)
10.1016/0092-8674(89)90925-2
VANBERKEL, TJC, THE EFFECT OF CA2+ AND TRIFLUOPERAZINE ON THE PROCESSING OF HUMAN ACETYLATED LOW-DENSITY LIPOPROTEIN BY NON-PARENCHYMAL LIVER-CELLS, FEBS LETTERS 132: 61 (1981). (10.1016/0014-5793(81)80427-9) / FEBS LETTERS (1981)
WILENTZ, J.R., PLATELET ACCUMULATION IN EXPERIMENTAL ANGIOPLASTY - TIME COURSE AND RELATION TO VASCULAR INJURY, CIRCULATION 75: 636 (1987). (10.1161/01.CIR.75.3.636) / CIRCULATION (1987)
WILSON, J.M., CORRECTION OF THE GENETIC-DEFECT IN HEPATOCYTES FROM THE WATANABE HERITABLE HYPERLIPIDEMIC RABBIT, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 4421 (1988). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1988)
10.1126/science.2734614

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:10 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 5:20 a.m.)
Indexed	5 days, 2 hours ago (Aug. 26, 2025, 2:23 a.m.)
Issued	34 years, 11 months ago (Sept. 14, 1990)
Published	34 years, 11 months ago (Sept. 14, 1990)
Published Print	34 years, 11 months ago (Sept. 14, 1990)

Funders 0

None

BibTeX

@article{Nabel_1990, title={Site-Specific Gene Expression in Vivo by Direct Gene Transfer into the Arterial Wall}, volume={249}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2119055}, DOI={10.1126/science.2119055}, number={4974}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Nabel, Elizabeth G. and Plautz, Gregory and Nabel, Gary J.}, year={1990}, month=sep, pages={1285–1288} }

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