DOI: 10.1126/science.2116666. Metalloantibodies

Metalloantibodies

10.1126/science.2116666

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A metalloantibody has been constructed with a coordination site for metals in the antigen binding pocket. The Zn(II) binding site from carbonic anhydrase B was used as a model. Three histidine residues have been placed in the light chain complementarity determining regions of a single chain antibody molecule. In contrast to the native protein, the mutant displayed metal-dependent fluorescence-quenching behavior. This response was interpreted as evidence for metal binding in the three-histidine site with relative affinities in the order Cu(II) > Zn(II) > Cd(II). The presence of metal cofactors in immunoglobulins should facilitate antibody catalysis of redox and hydrolytic reactions.

Bibliography

Iverson, B. L., Iverson, S. A., Roberts, V. A., Getzoff, E. D., Tainer, J. A., Benkovic, S. J., & Lerner, R. A. (1990). Metalloantibodies. Science, 249(4969), 659â662.

Authors 7

Brent L. Iverson (first)
Sheila A. Iverson (additional)
Victoria A. Roberts (additional)
Elizabeth D. Getzoff (additional)
John A. Tainer (additional)
Stephen J. Benkovic (additional)
Richard A. Lerner (additional)

References 23 Referenced 93

10.1126/science.3140379
CHOTHIA, C, CONFORMATIONS OF IMMUNOGLOBULIN HYPERVARIABLE REGIONS, NATURE 342: 877 (1989). (10.1038/342877a0) / NATURE (1989)
GETZOFF, E.D., THE CHEMISTRY AND MECHANISM OF ANTIBODY-BINDING TO PROTEIN ANTIGENS, ADVANCES IN IMMUNOLOGY 43: 1 (1988). (10.1016/S0065-2776(08)60363-6) / ADVANCES IN IMMUNOLOGY (1988)
GOTTESMAN, M.E., TRANSCRIPTION ANTITERMINATION BY BACTERIOPHAGE LAMBDA-N GENE-PRODUCT, JOURNAL OF MOLECULAR BIOLOGY 140: 57 (1980). (10.1016/0022-2836(80)90356-3) / JOURNAL OF MOLECULAR BIOLOGY (1980)
GUSSOW, D, GENERATING BINDING ACTIVITIES FROM ESCHERICHIA-COLI BY EXPRESSION OF A REPERTOIRE OF IMMUNOGLOBULIN VARIABLE DOMAINS, COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY 54: 265 (1989). (10.1101/SQB.1989.054.01.033) / COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY (1989)
HERRON, J.N., 3-DIMENSIONAL STRUCTURE OF A FLUORESCEIN FAB COMPLEX CRYSTALLIZED IN 2-METHYL-2,4-PENTANEDIOL, PROTEINS-STRUCTURE FUNCTION AND GENETICS 5: 271 (1989). (10.1002/prot.340050404) / PROTEINS-STRUCTURE FUNCTION AND GENETICS (1989)
10.1126/science.2531466
HUTNIK, C.M., A TIME-RESOLVED FLUORESCENCE STUDY OF AZURIN AND METALLOAZURIN DERIVATIVES, BIOCHEMISTRY 28: 3935 (1989). (10.1021/bi00435a046) / BIOCHEMISTRY (1989)
IVERSON, S.A., A COMBINATORIAL SYSTEM FOR CLONING AND EXPRESSING THE CATALYTIC ANTIBODY REPERTOIRE IN ESCHERICHIA-COLI, COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY 54: 273 (1989). (10.1101/SQB.1989.054.01.034) / COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY (1989)
KABAT E.A. SEQUENCES PROTEINS I (1987).
KANNAN, K.K., CRYSTAL-STRUCTURE OF HUMAN ERYTHROCYTE CARBONIC-ANHYDRASE B - 3-DIMENSIONAL STRUCTURE AT A NOMINAL 2.2-A RESOLUTION .2., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 72: 51 (1975). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1975)
LAKOWICZ J.R. PRINCIPLES FLUORESCE (1983).
LERNER, R.A., CHEMTRACTS. ORGANIC CHEMISTRY 3: 1 (1990). / CHEMTRACTS. ORGANIC CHEMISTRY (1990)
LINDSKOG, S, METAL-BINDING PROPERTIES OF HUMAN ERYTHROCYTE CARBONIC ANHYDRASES, BIOCHIMICA ET BIOPHYSICA ACTA 85: 462 (1964). / BIOCHIMICA ET BIOPHYSICA ACTA (1964)
10.1093/nar/14.24.9679
ORLANDI, R, CLONING IMMUNOGLOBULIN VARIABLE DOMAINS FOR EXPRESSION BY THE POLYMERASE CHAIN-REACTION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86: 3833 (1989). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1989)
PETRICH, J.W., INTERNAL MOTION AND ELECTRON-TRANSFER IN PROTEINS - A PICOSECOND FLUORESCENCE STUDY OF 3 HOMOLOGOUS AZURINS, BIOCHEMISTRY 26: 2711 (1987). (10.1021/bi00384a010) / BIOCHEMISTRY (1987)
Roberts, V. A., Proceedings of the National Academy of Sciences of the United States of America 87: 6654 (1990). / Proceedings of the National Academy of Sciences of the United States of America (1990)
SASTRY, L, CLONING OF THE IMMUNOLOGICAL REPERTOIRE IN ESCHERICHIA-COLI FOR GENERATION OF MONOCLONAL CATALYTIC ANTIBODIES - CONSTRUCTION OF A HEAVY-CHAIN VARIABLE REGION-SPECIFIC CDNA LIBRARY, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86: 5728 (1989). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1989)
SCANDELLA, D, J CELL BIOCH B 9: 203 (1985). / J CELL BIOCH B (1985)
10.1021/cen-v068n018.p026
STEINER, R.F., INTERACTION OF GROUND AND EXCITED STATES OF INDOLE DERIVATIVES WITH ELECTRON SCAVENGERS, JOURNAL OF PHYSICAL CHEMISTRY 73: 4130 (1969). (10.1021/j100846a015) / JOURNAL OF PHYSICAL CHEMISTRY (1969)
WARD, E.S., BINDING ACTIVITIES OF A REPERTOIRE OF SINGLE IMMUNOGLOBULIN VARIABLE DOMAINS SECRETED FROM ESCHERICHIA-COLI, NATURE 341: 544 (1989). (10.1038/341544a0) / NATURE (1989)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:10 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 5:24 a.m.)
Indexed	1 month, 3 weeks ago (July 8, 2025, 10:07 a.m.)
Issued	35 years ago (Aug. 10, 1990)
Published	35 years ago (Aug. 10, 1990)
Published Print	35 years ago (Aug. 10, 1990)

Funders 0

None

BibTeX

@article{Iverson_1990, title={Metalloantibodies}, volume={249}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2116666}, DOI={10.1126/science.2116666}, number={4969}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Iverson, Brent L. and Iverson, Sheila A. and Roberts, Victoria A. and Getzoff, Elizabeth D. and Tainer, John A. and Benkovic, Stephen J. and Lerner, Richard A.}, year={1990}, month=aug, pages={659–662} }

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