DOI: 10.1126/science.2111585. Platelet Coagulation Factor XI <sub>a</sub> -Inhibitor, a Form of Alzheimer Amyloid Precursor Protein

Platelet Coagulation Factor XI a -Inhibitor, a Form of Alzheimer Amyloid Precursor Protein

10.1126/science.2111585

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

An inhibitor of coagulation factor XI a was purified from serum-free conditioned medium of HepG2 liver cells. Platelets stimulated with thrombin or calcium ionophore (A23187) secrete a protein functionally and immunologically identical to the inhibitor, implying a role for this inhibitor in hemostasis. Analysis of the amino-terminal amino acid sequence and immunologic reactivity showed the inhibitor to be a truncated form of the Alzheimer's amyloid precursor protein that contains a Kunitz-type serine protease inhibitor domain and at least a portion of the amyloid β protein. It inhibits factor XI a and trypsin with a K i of 450 ± 50 pM and 20 ± 10 pM, respectively. Heparin (1 unit/ml) did not significantly effect inhibition of trypsin, but inhibition of XI a was 15 times greater ( K i = 25 ± 15 pM) in the presence of heparin.

Bibliography

Smith, R. P., Higuchi, D. A., & Broze, G. J. (1990). Platelet Coagulation Factor XI a -Inhibitor, a Form of Alzheimer Amyloid Precursor Protein. Science, 248(4959), 1126â1128.

Authors 3

Raymond P. Smith (first)
Darryl A. Higuchi (additional)
George J. Broze (additional)

References 29 Referenced 236

ANTONINI, E, MULTIPLE INTERMEDIATES IN THE REACTION OF BOVINE BETA-TRYPSIN WITH BOVINE PANCREATIC TRYPSIN-INHIBITOR (KUNITZ), BIOPOLYMERS 22: 363 (1983). (10.1002/bip.360220147) / BIOPOLYMERS (1983)
BROZE, G.J., MONOCLONAL ANTI-HUMAN FACTOR-VII ANTIBODIES - DETECTION IN PLASMA OF 2ND PROTEIN ANTIGENICALLY AND GENETICALLY RELATED TO FACTOR-VII, JOURNAL OF CLINICAL INVESTIGATION 76: 937 (1985). (10.1172/JCI112093) / JOURNAL OF CLINICAL INVESTIGATION (1985)
BROZE, G.J., ISOLATION OF THE TISSUE FACTOR INHIBITOR PRODUCED BY HEPG2 HEPATOMA-CELLS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 84: 1886 (1987). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1987)
BROZE, G.J., ISOLATION OF THE LIPOPROTEIN ASSOCIATED COAGULATION INHIBITOR PRODUCED BY HEPG2 (HUMAN HEPATOMA) CELLS USING BOVINE FACTOR-XA AFFINITY-CHROMATOGRAPHY, THROMBOSIS RESEARCH 48: 253 (1987). (10.1016/0049-3848(87)90422-1) / THROMBOSIS RESEARCH (1987)
CHASE, T, METHODS IN ENZYMOLOGY 19: 20 (1970). (10.1016/0076-6879(70)19004-5) / METHODS IN ENZYMOLOGY (1970)
10.1126/science.2569763
EDELHOCH, H, SPECTROSCOPIC DETERMINATION OF TRYPTOPHAN AND TYROSINE IN PROTEINS, BIOCHEMISTRY 6: 1948 (1967). (10.1021/bi00859a010) / BIOCHEMISTRY (1967)
10.1146/annurev.bi.53.070184.004215
ISHII, S.I., IMMOBILIZED ANHYDROTRYPSIN AS A SPECIFIC AFFINITY ADSORBENT FOR TRYPTIC PEPTIDES, METHODS IN ENZYMOLOGY 91: 378 (1983). (10.1016/S0076-6879(83)91035-2) / METHODS IN ENZYMOLOGY (1983)
JOACHIM, C.L., PROTEIN CHEMICAL AND IMMUNOCYTOCHEMICAL STUDIES OF MENINGOVASCULAR BETA-AMYLOID PROTEIN IN ALZHEIMERS-DISEASE AND NORMAL AGING, BRAIN RESEARCH 474: 100 (1988). (10.1016/0006-8993(88)90673-7) / BRAIN RESEARCH (1988)
10.1038/325733a0
KITAGUCHI, N, NOVEL PRECURSOR OF ALZHEIMERS-DISEASE AMYLOID PROTEIN SHOWS PROTEASE INHIBITORY ACTIVITY, NATURE 331: 530 (1988). (10.1038/331530a0) / NATURE (1988)
10.1038/227680a0
10.1073/pnas.82.12.4245
MILETICH, J.P., PROPERTIES OF FACTOR-XA BINDING-SITE ON HUMAN PLATELETS, JOURNAL OF BIOLOGICAL CHEMISTRY 253: 6908 (1978). (10.1016/S0021-9258(17)38006-7) / JOURNAL OF BIOLOGICAL CHEMISTRY (1978)
NOVOTNY, W.F., PURIFICATION AND CHARACTERIZATION OF THE LIPOPROTEIN-ASSOCIATED COAGULATION INHIBITOR FROM HUMAN-PLASMA, JOURNAL OF BIOLOGICAL CHEMISTRY 264: 18832 (1989). (10.1016/S0021-9258(18)51542-8) / JOURNAL OF BIOLOGICAL CHEMISTRY (1989)
OLTERSDORF, T, THE SECRETED FORM OF THE ALZHEIMERS AMYLOID PRECURSOR PROTEIN WITH THE KUNITZ DOMAIN IS PROTEASE NEXIN-II, NATURE 341: 144 (1989). (10.1038/341144a0) / NATURE (1989)
PALMERT, M.R., SOLUBLE DERIVATIVES OF THE BETA-AMYLOID PROTEIN-PRECURSOR OF ALZHEIMERS-DISEASE ARE LABELED BY ANTISERA TO THE BETA-AMYLOID PROTEIN, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 165: 182 (1989). (10.1016/0006-291X(89)91052-8) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1989)
PALMERT, M.R., THE BETA-AMYLOID PROTEIN-PRECURSOR OF ALZHEIMER-DISEASE HAS SOLUBLE DERIVATIVES FOUND IN HUMAN-BRAIN AND CEREBROSPINAL-FLUID, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86: 6338 (1989). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1989)
PONTE, P, A NEW A4-AMYLOID MESSENGER-RNA CONTAINS A DOMAIN HOMOLOGOUS TO SERINE PROTEINASE-INHIBITORS, NATURE 331: 525 (1988). (10.1038/331525a0) / NATURE (1988)
SCHMAIER, A.H., HEMOSTASIS THROMBOSI: 18 (1987). / HEMOSTASIS THROMBOSI (1987)
SELKOE, D.J., J NEUROCHEM 146: 1920 (1986). / J NEUROCHEM (1986)
SELKOE, D.J., BETA-AMYLOID PRECURSOR PROTEIN OF ALZHEIMER-DISEASE OCCURS AS 110-KILODALTON TO 135-KILODALTON MEMBRANE-ASSOCIATED PROTEINS IN NEURAL AND NONNEURAL TISSUES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 7341 (1988). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1988)
SNOW, A.D., SULFATED GLYCOSAMINOGLYCANS IN ALZHEIMERS-DISEASE, HUMAN PATHOLOGY 18: 506 (1987). (10.1016/S0046-8177(87)80036-9) / HUMAN PATHOLOGY (1987)
SOONS, H, THE EFFECT OF PLATELETS IN THE ACTIVATION OF HUMAN-BLOOD COAGULATION FACTOR-IX BY FACTOR-XIA, BLOOD 68: 140 (1986). (10.1182/blood.V68.1.140.140) / BLOOD (1986)
TOLLEFSEN, D.M., INDUCTION OF PLATELET-RELEASE REACTION BY PHYTOHEMAGGLUTININ, JOURNAL OF CLINICAL INVESTIGATION 53: 211 (1974). (10.1172/JCI107540) / JOURNAL OF CLINICAL INVESTIGATION (1974)
VANNOSTRAND, W.E., PROTEASE NEXIN-II, A POTENT ANTI-CHYMOTRYPSIN, SHOWS IDENTITY TO AMYLOID BETA-PROTEIN PRECURSOR, NATURE 341: 546 (1989). (10.1038/341546a0) / NATURE (1989)
WALSH, P.N., REGULATION OF FACTOR-XIA ACTIVITY BY PLATELETS AND ALPHA-1-PROTEASE INHIBITOR, JOURNAL OF CLINICAL INVESTIGATION 80: 1578 (1987). (10.1172/JCI113244) / JOURNAL OF CLINICAL INVESTIGATION (1987)
10.1016/0092-8674(89)90177-3

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 6:10 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 4:06 a.m.)
Indexed	11 months, 2 weeks ago (Sept. 12, 2024, 3:59 p.m.)
Issued	35 years, 2 months ago (June 1, 1990)
Published	35 years, 2 months ago (June 1, 1990)
Published Print	35 years, 2 months ago (June 1, 1990)

Funders 0

None

BibTeX

@article{Smith_1990, title={Platelet Coagulation Factor XI a -Inhibitor, a Form of Alzheimer Amyloid Precursor Protein}, volume={248}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2111585}, DOI={10.1126/science.2111585}, number={4959}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Smith, Raymond P. and Higuchi, Darryl A. and Broze, George J.}, year={1990}, month=jun, pages={1126–1128} }

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